US4742002A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Test kit and method for determining the presence of blood in a specimen and for testing the effectiveness of peroxidase inactivating solution
Assignee: Helena Laboratories Corp
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Filing: 1984-01-16
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Granted: 1988-05-03
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Expires: 2004-01-16
Abstract
Abstract A test kit for determining the presence of occult blood is disclosed which includes an improved monitor system having a reagent test area including a metal salt with an oxidation potential sufficient to catalyze a chromogen reaction in the guaiac impregnated paper when exposed to a peroxide developing solution. A peroxidase inactivating test area is provided for verifying the effectiveness of a peroxidase inactivating solution comprising guanidine hydrochloride and ethylenediaminetetraacetic acid. An improved method of testing occult blood is also disclosed wherein a peroxidase inactivating solution is applied to a monitor and a sample area just prior to application of the developing solution.
Inventors
- 👤 Philip A. Guadagno
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 03:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/528; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
73 words
A test kit for determining the presence of blood in a specimen comprising slide means for receiving a sample of the specimen in at least one sample test area thereof, and a second peroxidase inactivating solution test area formed on said slide with means on said slide having a deposit of chromogen reagent and peroxidase in a location spaced from the sample test area to test the effectiveness of a peroxidase inactivating solution.
Claim 2
Dependent Claim
73 words
A test kit for determining the presence of blood in a specimen comprising slide means for receiving a sample of the specimen in at least one sample test area thereof, and a second peroxidase inactivating solution test area formed on said slide with means on said slide having a deposit of chromogen reagent and peroxidase in a location spaced from the sample test area to test the effectiveness of a peroxidase inactivating solution.
Claim 3
Dependent Claim
12 words
The test kit of claim 1 wherein said peroxidase is vegetable peroxidase.
Claim 4
Dependent Claim
26 words
The test kit of claim 1 wherein a vegetable peroxidase inactivating solution is applied to the sample test area and the peroxidase inactivating solution test area.
Claim 5
Dependent Claim
20 words
The test kit of claim 1 wherein said peroxidase inactivating solution is a solution of guanidine hydrochloride and ethylenediaminetetraacetic acid.
Claim 6
Dependent Claim
22 words
The test kit of claim 4 wherein said solution of guanidine hydrochloride and ethylenediaminetetraacetic acid are combined at a ratio of 2:1.
Claim 7
Independent Claim
114 words
A method of determining the presence of blood in a specimen with a test slide carrying a test reagent comprising: applying a sample of the specimen to be tested on a first portion of the test slide, said test slide having a deposit of peroxidase in a second portion thereof; washing the first and second portions with a peroxidase inactivating solution; and applying a developing solution to the first and second portions which will cause blood in the specimen to catalyze a chromogen reaction in the test reagent and will only cause the peroxidase in the second portion to catalyze a chromogen reaction in the test reagent if the peroxidase inactivating solution is ineffective.
Claim 8
Independent Claim
114 words
A method of determining the presence of blood in a specimen with a test slide carrying a test reagent comprising: applying a sample of the specimen to be tested on a first portion of the test slide, said test slide having a deposit of peroxidase in a second portion thereof; washing the first and second portions with a peroxidase inactivating solution; and applying a developing solution to the first and second portions which will cause blood in the specimen to catalyze a chromogen reaction in the test reagent and will only cause the peroxidase in the second portion to catalyze a chromogen reaction in the test reagent if the peroxidase inactivating solution is ineffective.
Claim 9
Dependent Claim
16 words
The method of claim 6 wherein said peroxidase in the second portion is a vegetable peroxidase.
Claim 10
Dependent Claim
49 words
The method of claim 6 wherein said test slide has a reagent test area in a third portion comprising a deposit of a metal salt with an oxidation potential sufficient to catalyze a chromogen reaction in the test reagent when said developing solution is applied to the test slide.
Claim 11
Dependent Claim
45 words
The method of claim 6 wherein the test slide has a deposit of lead acetate on a third portion spaced from the first portion which will catalyze a chromogen reaction in the test reagent unless either the test reagent or developing solution are not functioning.
Claim 12
Dependent Claim
45 words
The method of claim 6 wherein the test slide has a deposit of copper cyanide on a third portion spaced from the first portion which will catalyze a chromogen reaction in the test reagent unless either the test reagent or developing solution are not functioning.
Claim 13
Dependent Claim
36 words
The method of claim 6 wherein a plurality of samples are applied at a plurality of spaced locations on the first portion of the test slide to permit a plurality of samples to be tested simultaneously.
Claim 14
Dependent Claim
19 words
The method of claim 6 wherein the peroxidase inactivating solution is a solution of guanidine hydrochloride and ethylenediaminetetraacetic acid.
References Cited (Backward Citations)
US4541987A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-03 | 1991-11-02 | 1992-05-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-03 | 1995-11-02 | 1996-05-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-03 | 1999-11-02 | 2000-05-03 | $7700.00 | Window Closed / Lapsed |