US4751185A
Expired / Lapsed
US
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Analysis method for hop bittering components
Assignee: Shimadzu Corp
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Filing: 1986-09-15
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Granted: 1988-06-14
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Expires: 2006-09-15
Abstract
Abstract An analysis method for hop bittering components which comprises a step of separating and analysing hop bittering components by liquid chromatography, characterized by trapping hop bittering components contained in a sample to be measured in a precolumn consisting of an ester type stationary phase for reversed phase chromatography by passing the sample through the precolumn by means of an acidic aqueous solution containing a polar organic solvent, introducing an eluent for reversed phase chromatography capable of eluting the bittering components into the precolumn, and subjecting the eluate to reversed phase liquid chromatography; and an analysis equipment which is suitable for conducting the above method.
Inventors
- 👤 Miyoko Ono
- 👤 Youichi Kakudo
- 👤 Yasuo Ishida
- 👤 Suehiro Ueda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/02; G01N33/14; G01N33/146; G01N30/00; G01N30/02; G01N30/04; G01N30/06; G01N30/08; G01N2030/085
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
103 words
In a method for analyzing hop bittering components in hop based beverages which comprises separating hop bittering components from a hop based beverage and analyzing the separated components by liquid chromatography, the improvement comprising trapping the hop bittering components contained in a sample to be measured in a precolumn consisting essentially of an ester type stationary phase for reversed phase chromatography by passing the sample through the precolumn in an acidic aqueous solution containing a polar organic solvent, introducing an eluent for reversed phase chromatography capable of eluting the bittering components into the precolumn, and subjecting the eluate to reversed phase liquid chromatography.
Claim 2
Dependent Claim
103 words
In a method for analyzing hop bittering components in hop based beverages which comprises separating hop bittering components from a hop based beverage and analyzing the separated components by liquid chromatography, the improvement comprising trapping the hop bittering components contained in a sample to be measured in a precolumn consisting essentially of an ester type stationary phase for reversed phase chromatography by passing the sample through the precolumn in an acidic aqueous solution containing a polar organic solvent, introducing an eluent for reversed phase chromatography capable of eluting the bittering components into the precolumn, and subjecting the eluate to reversed phase liquid chromatography.
Claim 3
Dependent Claim
15 words
The analysis method as claimed in claim 1, wherein the hop based beverage is beer.
Claim 4
Dependent Claim
20 words
The analysis method as claimed in claim 1, wherein the sample to be measured is hops or a hop product.
Claim 5
Dependent Claim
27 words
The analysis method as claimed in claim 1, wherein the ester type stationary phase for reversed phase chromatography is a stationary phase comprising an acrylate type resin.
Claim 6
Dependent Claim
28 words
The analysis method as claimed in claim 1, wherein the ester type stationary phase for reversed phase chromatography is a stationary phase comprising a tetramethylolmethane triacrylate type resin.
Claim 7
Dependent Claim
38 words
The analysis method as claimed in claim 1, wherein the acidic aqueous solution containing a polar organic solvent is an aqueous solution having a pH of 2-4 containing approximately 20-40% by volume of a lower alcohol or acetonitrile.
Claim 8
Dependent Claim
23 words
The analysis method as claimed in claim 1, wherein the eluent for reversed phase chromatography is a polar mobile phase containing an alkylammonium.
References Cited (Backward Citations)
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Cited
US4302479A
Cited
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Cited
US4228192A
Cited
US4447328A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-06-14 | 1991-12-14 | 1992-06-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-06-14 | 1995-12-14 | 1996-06-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-06-14 | 1999-12-14 | 2000-06-14 | $7700.00 | Window Closed / Lapsed |