US4777762A
Expired / Lapsed
US
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Desiccated analogs of botanic seed
Assignee: Monsanto Co
•
Filing: 1986-12-24
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Granted: 1988-10-18
•
Expires: 2006-12-24
Abstract
Abstract Methods and materials are provided for desiccated analogs of botanic seeds which are created by removing a portion of the water by slow or fast drying so that the plant tissue is no longer saturated with water. In a second embodiment, the meristematic tissue is encapsulated in a gel or polymer followed by dehydration. In another embodiment, the meristematic tissue is dehydrated prior to encapsulation in a gel. Protectants can be incorporated in the gel capsule. Meristematic tissue can be isolated from, for example, somatic embryos, zygotic and germ line sources.
Inventors
- 👤 M. Keith Redenbaugh
- 👤 David Slade
- 👤 Jo A. Fujii
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 10:39
CPC Classifications
A; A01; A01C; A01C1/00; A01C1/06; A01H; A01H4/00; Y; Y10; Y10S; Y10S47/00; Y10S47/09
Claims Analysis
Total Claims: 49
Claim 1
Independent Claim
12 words
A process for creating an analog to natural botanic seed, which comprises:
Claim 2
Dependent Claim
12 words
A process for creating an analog to natural botanic seed, which comprises:
Claim 3
Dependent Claim
17 words
isolating totipotent meristematic tissue, which tissue has the potential to differentiate to produce an entire plant body;
Claim 4
Dependent Claim
17 words
isolating totipotent meristematic tissue, which tissue has the potential to differentiate to produce an entire plant body;
Claim 5
Dependent Claim
19 words
encapsulating said isolated meristematic tissue in a hydrated gel capsule which will permit development of said plant body; and
Claim 6
Dependent Claim
19 words
encapsulating said isolated meristematic tissue in a hydrated gel capsule which will permit development of said plant body; and
Claim 7
Dependent Claim
16 words
dehydrating said gel capsule until the saturation level of the meristematic tissue is less than 99.9%.
Claim 8
Dependent Claim
16 words
dehydrating said gel capsule until the saturation level of the meristematic tissue is less than 99.9%.
Claim 9
Dependent Claim
18 words
The process according to claim 1 wherein said meristematic tissue is encapsulated substantially free from botanic accessory structures.
Claim 10
Dependent Claim
15 words
The process according to claim 2 wherein the meristematic tissue is derived from somatic tissue.
Claim 11
Dependent Claim
30 words
The process according to claim 3 wherein the somatic tissue is from a plant selected from the group consisting of Medicago sativa L., Apium graveolens L., and Brassica camoestris L.
Claim 12
Dependent Claim
15 words
The process according to claim 1 wherein said meristematic tissue is derived from zygotic tissue.
Claim 13
Dependent Claim
16 words
The process according to claim 1 wherein said meristematic tissue is derived from germ line tissue.
Claim 14
Dependent Claim
20 words
The process according to claim 1 wherein said meristematic tissue is tissue which has been induced to form shoot meristems.
Claim 15
Dependent Claim
16 words
The process according to claim 1 wherein said gel comprises one or more distinct gel agents.
Claim 16
Dependent Claim
18 words
The process according to claim 1 wherein said gel further comprises biologically active adjuvants in bio-affecting concentrations thereof.
Claim 17
Dependent Claim
27 words
The process according to claim 1 wherein the gel is selected from the group consisting of sodium alginate, sodium alginate complexed with a calcium salt, and Terra-Sorb®.
Claim 18
Dependent Claim
26 words
The process according to claim 10 wherein a protectant is added to the gel, such protectant being selected from a group consisting of dimethylsulfoxide and dextran.
Claim 19
Dependent Claim
12 words
A process for creating an analog to natural botanic seed, which comprises:
Claim 20
Dependent Claim
12 words
A process for creating an analog to natural botanic seed, which comprises:
Claim 21
Dependent Claim
17 words
isolating totipotent meristematic tissue, which tissue has the potential to differentiate to produce an entire plant body;
Claim 22
Dependent Claim
17 words
isolating totipotent meristematic tissue, which tissue has the potential to differentiate to produce an entire plant body;
Claim 23
Dependent Claim
17 words
dehydrating said meristematic tissue such that the saturation level of the tissue is less than 99.9%; and,
Claim 24
Dependent Claim
17 words
dehydrating said meristematic tissue such that the saturation level of the tissue is less than 99.9%; and,
Claim 25
Dependent Claim
19 words
encapsulating said isolated, dehydrated meristematic tissue in a hydrated gel capsule which will permit development of said plant body.
Claim 26
Dependent Claim
19 words
encapsulating said isolated, dehydrated meristematic tissue in a hydrated gel capsule which will permit development of said plant body.
Claim 27
Dependent Claim
28 words
The process of claim 12 further comprising dehydrating said hydrated gel capsule containing dehydrated meristematic tissue such that the saturation level of said gel is less than 99.9%.
Claim 28
Dependent Claim
18 words
The process according to claim 12 wherein said meristematic tissue is encapsulated substantially free from botanic accessory structures.
Claim 29
Dependent Claim
15 words
The process according to claim 12 wherein the meristematic tissue is derived from somatic tissue.
Claim 30
Dependent Claim
30 words
The process according to claim 12 wherein the somatic tissue is from a plant selected from the group consisting of Medicago sativa L., Apium graveolens L., and Brassica campestris L.
Claim 31
Dependent Claim
15 words
The process according to claim 12 wherein said meristematic tissue is derived from zygotic tissue.
Claim 32
Dependent Claim
16 words
The process according to claim 12 wherein said meristematic tissue is derived from germ line tissue.
Claim 33
Dependent Claim
20 words
The process according to claim 12 wherein said meristematic tissue is tissue which has been induced to form shoot meristems.
Claim 34
Dependent Claim
16 words
The process according to claim 12 wherein said gel comprises one or more distinct gel agents.
Claim 35
Dependent Claim
18 words
The process according to claim 12 wherein said gel further comprises biologically active adjuvants in bio-affecting concentrations thereof.
Claim 36
Dependent Claim
17 words
The process according to claim 12 wherein the gel is sodium alginate, complexed with a calcium salt.
Claim 37
Dependent Claim
12 words
The process according to claim 12 wherein the gel is fumed silica.
Claim 38
Dependent Claim
54 words
An analog to natural botanic seed which comprises: meristematic tissue having the potential to differentiate into an entire plant body which is encapsulated in a hydrated gel capsule which will permit development of said body and which capsule is subsequently dehydrated such that the saturation level of such meristematic tissue is less than 99.9%.
Claim 39
Dependent Claim
54 words
An analog to natural botanic seed which comprises: meristematic tissue having the potential to differentiate into an entire plant body which is encapsulated in a hydrated gel capsule which will permit development of said body and which capsule is subsequently dehydrated such that the saturation level of such meristematic tissue is less than 99.9%.
Claim 40
Dependent Claim
51 words
An analog to natural botanic seed which comprises: meristematic tissue having the potential to differentiate into an entire plant body which tissue is dehydrated such that the saturation level is less than 99.9% and which tissue is subsequently encapsulated in a hydrated gel capsule which will permit development of said body.
Claim 41
Dependent Claim
51 words
An analog to natural botanic seed which comprises: meristematic tissue having the potential to differentiate into an entire plant body which tissue is dehydrated such that the saturation level is less than 99.9% and which tissue is subsequently encapsulated in a hydrated gel capsule which will permit development of said body.
Claim 42
Dependent Claim
25 words
The analog according to claim 24 wherein said meristematic tissue is tissue selected from the group consisting of somatic tissue, zygotic tissue and germline tissue.
Claim 43
Dependent Claim
16 words
The analog according to claim 24 wherein said gel comprises one or more distinct gel agents.
Claim 44
Dependent Claim
18 words
The analog according to claim 24 wherein said gel further comprises biologically active adjuvants in bio-affecting concentrations thereof.
Claim 45
Dependent Claim
27 words
The analog according to claim 24 wherein said gel is selected from the group consisting of sodium alginate, sodium alginate complexed with a calcium salt, and Terra-Sorb®.
Claim 46
Dependent Claim
25 words
The analog according to claim 25 wherein said meristematic tissue is tissue selected from the group consisting of somatic tissue, zygotic tissue and germline tissue.
Claim 47
Dependent Claim
16 words
The analog according to claim 25 wherein said gel comprises one or more distinct gel agents.
Claim 48
Dependent Claim
18 words
The analog according to claim 25 wherein said gel further comprises biologically active adjuvants in bio-affecting concentrations thereof.
Claim 49
Dependent Claim
26 words
The analog according to claim 25 wherein said gel is selected from the group consisting of sodium alginate complexed with a calcium salt and fumed silica.
References Cited (Backward Citations)
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