Patent US5690587A successfully refreshed from Google Patents.
US4780423A
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Heterogeneous fluorescence assays using controlled pore glass particles
Assignee: Bayer Corp
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Filing: 1986-12-09
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Granted: 1988-10-25
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Expires: 2006-12-09
Abstract
Abstract Improved heterogeneous fluorescence assays are provided which employ solid supports comprising controlled pore glass particles which are substantially transparent at both the wavelength used to excite the fluorescent probe used in the assay and at the emission wavelength of the probe. The assays achieve sensitivities comparable to those achieved with radioimmunoassays without the need to concentrate the particles prior to the fluorescent measurement as in prior art heterogeneous fluorescence assays employing other types of solid supports.
Inventors
- 👤 Barry I. Bluestein
- 👤 A. Judy Famulare
- 👤 Thomas E. Worthy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 17:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/551; G01N33/552; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
71 words
In a heterogeneous fluorescence assay wherein a ligand/ligand-binding-partner complex is formed on a solid support, the ligand or ligand-binding-partner including a fluorescent probe, and fluorescence is measured by exciting the probe with light of a first wavelength and sensing the light emitted by the probe at a second wavelength, the improvement comprising using as the solid support controlled pore glass particles which are substantially transparent at the first and second wavelengths.
Claim 2
Dependent Claim
71 words
In a heterogeneous fluorescence assay wherein a ligand/ligand-binding-partner complex is formed on a solid support, the ligand or ligand-binding-partner including a fluorescent probe, and fluorescence is measured by exciting the probe with light of a first wavelength and sensing the light emitted by the probe at a second wavelength, the improvement comprising using as the solid support controlled pore glass particles which are substantially transparent at the first and second wavelengths.
Claim 3
Dependent Claim
54 words
The heterogeneous fluorescence assay of claim 1 wherein the controlled pore glass particles have a mean pore size of between about 400 angstroms and about 800 angstroms, a mean diameter of between about 0.7 microns and about 3.0 microns, and a mean surface area to mass ratio of at least 40 meter 2 /gram.
Claim 4
Dependent Claim
34 words
The heterogenous fluorescence assay of claim 2 wherein the controlled pore glass particles have a mean surface area to mass ratio of between about 40 meter 2 /gram and about 125 meter 2 /gram.
Claim 5
Dependent Claim
55 words
The heterogeneous fluorescence assay of claim 3 wherein the controlled pore glass particles are composed of borosilicate glass, have a mean pore size of about 550 angstroms, a mean diameter of about 1.5 microns, and a mean surface area to mass ratio of between about 40 meter 2 /gram and about 100 meter 2 /gram.
Claim 6
Dependent Claim
19 words
The heterogeneous fluorescence assay of claim 1 wherein the fluorescence of the probe is measured using front face epifluorometry.
Claim 7
Dependent Claim
18 words
The heterogeneous fluorescence assay of claim 5 wherein the epifluorometry is performed on the particles suspended in buffer.
Claim 8
Dependent Claim
19 words
The heterogeneous fluorescence assay of claim 1 wherein the ligand is an antigen and the ligand-binding-partner is an antibody.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-25 | 1992-04-25 | 1992-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-25 | 1996-04-25 | 1996-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-25 | 2000-04-25 | 2000-10-25 | $7700.00 | Window Closed / Lapsed |