US4816567A
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US
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Recombinant immunoglobin preparations
Assignee: Genentech Inc
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Filing: 1983-04-08
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Granted: 1989-03-28
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Expires: 2003-04-08
Abstract
Abstract Altered and native immunoglobulins, including constant-variable region chimeras, are prepared in recombinant cell culture. The immunoglobulins contain variable regions which are immunologically capable of binding predetermined antigens. Methods are provided for refolding directly expressed immunoglobulins into immunologically active form.
Inventors
- 👤 Shmuel Cabilly
- 👤 Herbert L. Heyneker
- 👤 William E. Holmes
- 👤 Arthur D. Riggs
- 👤 Ronald B. Wetzel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:01
CPC Classifications
A; A01; A01N; A01N65/00; A01N65/40; A01N65/44; C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; A01N25/00; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/30; C07K16/3007; C07K16/46; C07K16/461; C07K16/462; C12N15/79; C12N15/80; C12N15/81; C07K2317/00; C07K2317/20; C07K2317/24; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/30; Y; Y10; Y10S; Y10S530/00; Y10S530/866; Y10S530/867; Y10S930/00; Y10S930/01; Y10S930/30
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
112 words
A method comprising (a) preparing a DNA sequence encoding a chimeric immunoglobulin heavy or light chain having specificity for a particular known antigen wherein a constant region is homologous to the corresponding constant region of an antibody of a first mammalian species and a variable region thereof is homologous to the variable region of an antibody derived from a second, different mammalian species; (b) inserting the sequence into a replicable expression vector operably linked to a suitable promoter compatible with a host cell; (c) transforming the host cell with the vector of (b); (d) culturing the host cell; and (e) recovering the chimeric heavy or light chain from the host cell culture.
Claim 2
Independent Claim
112 words
A method comprising (a) preparing a DNA sequence encoding a chimeric immunoglobulin heavy or light chain having specificity for a particular known antigen wherein a constant region is homologous to the corresponding constant region of an antibody of a first mammalian species and a variable region thereof is homologous to the variable region of an antibody derived from a second, different mammalian species; (b) inserting the sequence into a replicable expression vector operably linked to a suitable promoter compatible with a host cell; (c) transforming the host cell with the vector of (b); (d) culturing the host cell; and (e) recovering the chimeric heavy or light chain from the host cell culture.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein the first mammalian species is human.
Claim 4
Dependent Claim
54 words
A composition comprising a chimeric immunoglobulin heavy or light chain having specificity for a particular known antigen having a constant region homologous to a corresponding constant region of an antibody of a first mammalian species and a variable region homologous to a variable region of an antibody derived from a second, different mammalian species.
Claim 5
Dependent Claim
54 words
A composition comprising a chimeric immunoglobulin heavy or light chain having specificity for a particular known antigen having a constant region homologous to a corresponding constant region of an antibody of a first mammalian species and a variable region homologous to a variable region of an antibody derived from a second, different mammalian species.
Claim 6
Dependent Claim
15 words
The chimeric heavy or light chain of clain 3 wherein the constant region is human.
Claim 7
Dependent Claim
15 words
The chimeric heavy or light chain of clain 3 wherein the constant region is human.
Claim 8
Dependent Claim
71 words
A replicable expression vector comprising DNA operably linked to a promoter compatible with a suitable host cell, said DNA encoding a chimeric immunoglobulin heavy or light chain having specificity for a particular known antigen and having a constant region homologous to a corresponding region of an antibody of a first mammalian species and a variable region homologous to a variable region of an antibody derived from a second, different mammalian species.
Claim 9
Dependent Claim
71 words
A replicable expression vector comprising DNA operably linked to a promoter compatible with a suitable host cell, said DNA encoding a chimeric immunoglobulin heavy or light chain having specificity for a particular known antigen and having a constant region homologous to a corresponding region of an antibody of a first mammalian species and a variable region homologous to a variable region of an antibody derived from a second, different mammalian species.
Claim 10
Dependent Claim
12 words
The vector of claim 5 wherein the first mammalian species is human.
Claim 11
Dependent Claim
10 words
Recombinant host cells transformed with the vector of claim 5.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-03-27 | 1992-09-26 | 1993-03-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-03-27 | 1996-09-26 | 1997-03-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-03-27 | 2000-09-26 | 2001-03-28 | $7700.00 | Window Closed / Lapsed |