US4824529A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Lipid membrane-based device
Assignee: Honeywell International Inc
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Filing: 1987-11-27
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Granted: 1989-04-25
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Expires: 2007-11-27
Abstract
Abstract A protected, lipid membrane-based device useful as a chemoreceptive transducer is provided. The device includes a porous, membrane-protective layer attached to an underlying, lipid membrane. Also provided is a process for using the protected device to determine the concentration of a specified chemical species. There is additionally provided a lipid membrane-based gas sensor. The sensor includes a gas-permeable, hydrated upper layer attached to an underlying lipid membrane. Furthermore, the invention includes a process for using the gas sensor to determine the concentration of an inorganic ion formed from an inorganic ion-forming gas.
Inventors
- 👤 Michael Thompson
- 👤 Ulrich J. Krull
- 👤 Krishna M. Kallury
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 04:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N31/00; G01N31/22; G01N31/223; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
95 words
A protected, lipid membrane-based device useful as a chemoreceptive transducer for determining the concentration of a specified chemical species, said device comprising: a porous, ion-permeable, hydratable, membrane-protective layer, the pores of which permit passage therethrough of said specified chemical species but block passage of a larger material from which an underlying lipid membrane is desirably shielded, and attached to said membrane-protective layer, an underlying, lipid membrane which controls ion permeability, said lipid membrane being modified by the incorporation of a complexing agent for selectively interacting with said specified chemical species to increase membrane ion permeability.
Claim 2
Dependent Claim
95 words
A protected, lipid membrane-based device useful as a chemoreceptive transducer for determining the concentration of a specified chemical species, said device comprising: a porous, ion-permeable, hydratable, membrane-protective layer, the pores of which permit passage therethrough of said specified chemical species but block passage of a larger material from which an underlying lipid membrane is desirably shielded, and attached to said membrane-protective layer, an underlying, lipid membrane which controls ion permeability, said lipid membrane being modified by the incorporation of a complexing agent for selectively interacting with said specified chemical species to increase membrane ion permeability.
Claim 3
Dependent Claim
13 words
The device of claim 1, wherein said membrane-protective layer is a mucous layer.
Claim 4
Dependent Claim
14 words
The device of claim 2, wherein said mucous layer is a polysaccharide, mucous layer.
Claim 5
Dependent Claim
16 words
The device of claim 3, wherein said polysaccharide, mucous layer comprises repeating units derived from 1,6-anhydro-β-D-glucopyranose.
Claim 6
Dependent Claim
30 words
The device of claim 1, wherein attachment between said membrane-protective layer and said lipid membrane, is through a crosslinker that comprises a protective layer-forming moiety and a lipid membrane-bonding group.
Claim 7
Dependent Claim
13 words
The device of claim 5, wherein said crosslinker further comprises a glycerophosphate moiety.
Claim 8
Dependent Claim
11 words
The device of claim 6, wherein said crosslinker is a glycophospholipid.
Claim 9
Dependent Claim
18 words
The device of claim 1, wherein said membrane-protective layer is attached to said lipid membrane by physical bonding.
Claim 10
Dependent Claim
18 words
The device of claim 1, wherein said membrane-protective layer is attached to said lipid membrane by hydrophobic bonding.
Claim 11
Dependent Claim
11 words
The device of claim 1, further comprising a lipid membrane-stabilizing support.
Claim 12
Dependent Claim
89 words
A process for determining the concentration of a selected chemical species in an aqueous electrolytic solution, said process comprising: (a) forming an electrochemical cell from said aqueous electrolytic solution and the device of claim 1; (b) applying an electrical potential difference across the modified lipid membrane of said device whereby said chemical species interacts with the membrane-incorporated complexing agent to produce an analytical signal based upon an increase in membrane ion permeability; (c) measuring said analytical signal; and (d) determining said chemical species concentration from the measured analytical signal.
Claim 13
Dependent Claim
11 words
The process of claim 11, wherein capacitance or admittance is measured.
Claim 14
Dependent Claim
9 words
The process of claim 11, wherein conductivity is measured.
Claim 15
Dependent Claim
64 words
A lipid membrane-based, gas sensor comprising a gas-permeable, hydrated, upper layer permeable to an inorganic ion-forming gas, and attached to an underlying lipid membrane, said lipid membrane including a complexing agent for selectively interacting with a specified inorganic ion formed by dissolution of said inorganic ion-forming gas in the hydrated, upper layer, to increase permeability of said lipid membrane to said specified inorganic ion.
Claim 16
Dependent Claim
64 words
A lipid membrane-based, gas sensor comprising a gas-permeable, hydrated, upper layer permeable to an inorganic ion-forming gas, and attached to an underlying lipid membrane, said lipid membrane including a complexing agent for selectively interacting with a specified inorganic ion formed by dissolution of said inorganic ion-forming gas in the hydrated, upper layer, to increase permeability of said lipid membrane to said specified inorganic ion.
Claim 17
Dependent Claim
15 words
The gas sensor of claim 14, wherein said hydrated, upper layer is a mucous layer.
Claim 18
Dependent Claim
15 words
The gas sensor of claim 15, wherein said mucous layer is a polysaccharide, mucous layer.
Claim 19
Dependent Claim
17 words
The gas sensor of claim 16, wherein said polysaccharide, mucous layer comprises repeating units derived from 1,6-anhydro-β-D-glucopyranose.
Claim 20
Dependent Claim
31 words
The gas sensor of claim 14, wherein attachment between said upper layer and said lipid membrane, is through a crosslinker that comprises an upper layer-forming moiety and a lipid membrane-bonding group.
Claim 21
Dependent Claim
14 words
The gas sensor of claim 18, wherein said crosslinker further comprises a glycerophosphate moiety.
Claim 22
Dependent Claim
12 words
The gas sensor of claim 19, wherein said crosslinker is a glycophospholipid.
Claim 23
Dependent Claim
19 words
The gas sensor of claim 14, wherein said upper layer is attached to said lipid membrane by physical bonding.
Claim 24
Dependent Claim
19 words
The gas sensor of claim 21, wherein said upper layer is attached to said lipid membrane by hydrophobic bonding.
Claim 25
Dependent Claim
12 words
The gas sensor of claim 14, further comprising a lipid membrane-stabilizing support.
Claim 26
Dependent Claim
96 words
A process for determining the concentration of a specified inorganic ion formed by dissolution of an inorganic ion-forming gas in an aqueous electrolytic solution, said process comprising (a) applying an electrical potential difference across the lipid membrane of the gas sensor of claim 14, whereby said inorganic ion interacts with the lipid membrane-incorporated, complexing agent to increase the permeability of said lipid membrane to said inorganic ion, thereby producing an analytical signal based upon the increased membrane ion permeability; (b) measuring said analytical signal; and (c) determining said inorganic ion concentration from the measured analytical signal.
Claim 27
Dependent Claim
11 words
The process of claim 24, wherein capacitance or admittance is measured.
Claim 28
Dependent Claim
9 words
The process of claim 24, wherein conductivity is measured.
References Cited (Backward Citations)
US4661235A
Cited
US4637861A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-04-24 | 1992-10-24 | 1993-04-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-24 | 1996-10-24 | 1997-04-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-24 | 2000-10-24 | 2001-04-25 | $7700.00 | Window Closed / Lapsed |