Monoclonal antibody specific for DNA.RNA hybrids
Abstract
Abstract A monoclonal antibody specific for DNA·RNA duplexes, particularly DNA·RNA heteropolymer duplexes, characterized by having cross-reactivity for binding to single- or double-stranded DNA or RNA as measured by competitive immunoassay of less than about 1:1000, and preferably less than 1:10,000, and an affinity for DNA·RNA heteropolymer duplexes greater than 10 9 L/mole. The monoclonal antibody is prepared by conventional somatic cell hybridization techniques wherein the host animal is preferably immunized with an immunogen comprising a random DNA·RNA heteropolymer. The antibody, particularly in a labeled form, is useful in the specific detection of DNA·RNA duplexes in a test medium such as a nucleic acid hybridization assay mixture.
Inventors
- 👤 Robert J. Carrico
Bibliographic Details
Claims Analysis
Total Claims: 4References Cited (Backward Citations)
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