US4837168A
Expired / Lapsed
US
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Immunoassay using colorable latex particles
Assignee: Janssen Pharmaceutica NV
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Filing: 1986-12-15
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Granted: 1989-06-06
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Expires: 2006-12-15
Abstract
Abstract New method for the detection of specific binding agents and their corresponding bindable substances by employing a label which is a latex particle which can be visually detected.
Inventors
- 👤 Nikolaas C. J. de Jaeger
- 👤 Marcel J. Monbaliu
- 👤 Marcus J. M. Noppe
- 👤 Frank J. Konings
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 19:24
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/58; G01N33/585; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/807; Y10S436/808; Y10S436/823; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
78 words
A method of qualitatively or quantitatively determining a component of a complex formed between at least one specific binding protein and its corresponding bindable substance which comprises labeling at least one component of said complex with a marker characterized in that the marker used consists of colorable latex particles which are chemically converted into colored latex particles after the formation of said complex and the determination is based on the color characteristics of the colored particles derived therefrom.
Claim 2
Independent Claim
78 words
A method of qualitatively or quantitatively determining a component of a complex formed between at least one specific binding protein and its corresponding bindable substance which comprises labeling at least one component of said complex with a marker characterized in that the marker used consists of colorable latex particles which are chemically converted into colored latex particles after the formation of said complex and the determination is based on the color characteristics of the colored particles derived therefrom.
Claim 3
Dependent Claim
33 words
A method according to claim 1 wherein the said colorable latex particles comprise recurrent units of a monomer carrying a moiety capable of oxidatively coupling with an amino compound to form a dye.
Claim 4
Dependent Claim
47 words
A method according to claim 1 wherein said colorable latex particles comprise recurrent units of a monomer represented by the formula ##STR14## wherein: R is hydrogen, halogen or C 1-4 alkyl; Q is a colour coupler group cabable of oxidatively coupling with an aromatic primary amino compound.
Claim 5
Dependent Claim
33 words
A method according to claim 3 wherein the aromatic primary amino coupound is a p-phenylene diamine and Q is selected from the group consisting of phenol, naphthol, pyrazolone, indazolone, acylacetamides and derivatives thereof.
Claim 6
Dependent Claim
130 words
A method according to claim 3 wherein the colorable latex comprises a polymer of the formula: ##STR15## wherein k is from 30 to 60 and y is from 40 to 70 w/w; or of the formula: ##STR16## wherein n is from 20 to 70, m is from 30 to 60, and p is from 0 to 40 w/w; or of the formula: ##STR17## wherein k is from 30 to 60, y is from 20 to 70, and z is from 0 to 40 w/w; or of the formula: ##STR18## wherein n is from 20 to 70, m is from 0 to 40, and p is from 30 to 60 w/w; or of the formula: ##STR19## wherein n is from 40 to 70 and m is from 30 to 60 w/w.
Claim 7
Dependent Claim
102 words
A method according to claim 3, comprising the steps of: (a) immobilizing the bindable substance on a solid support; (b) contacting the support containing the immobilized bindable substance with an aqeous suspension of colorable latex particles coated with binding proteins specific to said bindable substance; (c) separating said solid support from said aqueous suspension; (d) converting the colorable latex particles bound to said solid suport into colored particles by oxidatively coupling them with an aromatic primary amine compound; and (e) observing the degree of color formation on said solid support and relating said color formation to the presence of said bindable substance.
Claim 8
Dependent Claim
40 words
A method according to claim 6, wherein the said bindable substance is immobilized on the said solid support by allowing the said bindable substance to be bound by a specific binding protein which is immobilized on the said solid support.
Claim 9
Dependent Claim
132 words
A method according to claim 3, comprising the steps of: (a) immobilizing the bindable substance on a solid support; (b) contacting the support containing the immobilized bindable substance with a first binding protein specific to said bindable substance to form a complex therewith; (c) treating the support carrying the thus formed complex with an aqueous suspension of colorable latex particles coated with a secondary binding protein which is specific to the said first binding protein; (d) separating said solid support from said aqueous suspension; (e) converting the colorable latex particles bound to said solid support into colored particles by oxidatively coupling them with an aromatic primary amine compund; and (f) observing the degree of color formation on said solid support and relating said color formation to the presence of said bindable substance.
Claim 10
Dependent Claim
101 words
A method according to claim 3, comprising the steps of: (a) immobilizing the bindable substance on a solid support; (b) contacting the support containing the immobilized bindable substance with an aqueous suspension or colorable latex particles coated with binding proteins specific to said bindable substance; (c) separating said solid support from said aqueous suspension; (d) converting the colorable latex particles in said aqueous suspension into colored particles by oxidatively coupling them with an aromatic primary amine compound; and (e) observing the degree of color formation in said aqueous suspension and relating said color formation to the presence of said bindable substance.
Claim 11
Dependent Claim
131 words
A method according to claim 3, comprising the steps of: (a) immobilizing the bindable substance on a solid support; (b) contacting the support containing the immobilized bindable substance with a first binding protein specific to said bindable substance to form a complex therewith; (c) treating the support carrying the thus formed complex with an aqueous suspension of colorable latex particles coated with a secondary binding protein which is specific to the said first binding protein; (d) separating said solid support from said aqueous suspension; (e) converting the colorable latex particles in said aqueous suspension into colored particles by oxidatively coupling them with an aromatic primary amine compounds; and (f) observing the degree of color formation in said aqueous suspension and relating said color formation to the presence of said bindable substance.
Claim 12
Dependent Claim
22 words
Colorable latex particles coated with a specific binding protein, wherein said colorable latex particles can be chemically converted into colored latex particles.
Claim 13
Dependent Claim
22 words
Colorable latex particles coated with a specific binding protein, wherein said colorable latex particles can be chemically converted into colored latex particles.
Claim 14
Dependent Claim
54 words
Colorable latex particles coated with specific binding proteins, according to claim 11, wherein the particles comprise a polymer containing recurrent units of a monomer represented by the formula ##STR20## wherein: R is hydrogen, halogen of C 1-4 alkyl; Q is a colour coupler group capable of oxidatively coupling with an aromatic primary amino compound.
Claim 15
Dependent Claim
34 words
Colorable latex particles according to claim 12 wherein the aromatic primary amino compound is a p-phenylene diamine and Q is selected from the group consisting of phenol, naphthol, pyrazolone, indazolone, acylacetamides and derivatives thereof.
Claim 16
Dependent Claim
131 words
Colorable latex particles according to claim 11 wherein the colourable latex comprises a polymer of the formula: ##STR21## wherein k is from 30 to 60 and y is from 40 to 70 w/w; or of the formula: ##STR22## wherein n is from 20 to 70, m is from 30 to 60, and p is from 0 to 40 w/w; or of the formula: ##STR23## wherein k is from 30 to 60, y is from 20 to 70, and z is from 0 to 40 w/w; or of the formula ##STR24## wherein n is from 20 to 70, m is from 0 to 40, and p is from 30 to 60 w/w; or of the formula: ##STR25## wherein n is from 40 to 70 and m is from 30 to 60 w/w.
Claim 17
Dependent Claim
21 words
A test kit for the determination of a particular bindable substance which comprises the particles of claim 11 and ancillary reagents.
Claim 18
Dependent Claim
43 words
A test kit for the determination of a particular bindable substance which comprises a primary binding protein which is specific to said bindable substance and the particles of claim 11 in which the specific binding protein is specific to the primary binding protein.
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| 7.5 Years (Year 8) | 1996-06-05 | 1996-12-05 | 1997-06-06 | $3760.00 | Window Closed / Lapsed |
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