US4857474A
Expired / Lapsed
US
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Phycoerythrins useful in fluorescent conjugates
Assignee: University of California
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Filing: 1985-03-29
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Granted: 1989-08-15
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Expires: 2005-03-29
Abstract
Abstract This invention provides a class of phycoerythrins useful in diagnostic and detection protocols wherein a fluorescent label is required. The unique spectral properties of the phycoerythrins described herein provide for increased sensitivity and alternative uses in assays employing them.
Inventors
- 👤 John B. Waterbury
- 👤 Stanley W. Watson
- 👤 Alexander N. Glazer
- 👤 Linda J. Ong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 21:01
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; C; C07; C07K; C07K14/00; C07K14/195; C07K19/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
78 words
In a fluorescent immunoassay method employing as a reagent a fluorescent biliprotein isolated from a marine cyanobacterium of the genus Synechococcus, conjugated to a member of a specific binding pair selected from the group consisting of ligand and receptor the improvement which comprises employing a biliprotein having absorption maxima at about 542 and at about 492, a 542/492 ratio of less than about 2, a PUB/PEB ratio of greater than 0.36, and an emission maximum at 560 nm.
Claim 2
Dependent Claim
78 words
In a fluorescent immunoassay method employing as a reagent a fluorescent biliprotein isolated from a marine cyanobacterium of the genus Synechococcus, conjugated to a member of a specific binding pair selected from the group consisting of ligand and receptor the improvement which comprises employing a biliprotein having absorption maxima at about 542 and at about 492, a 542/492 ratio of less than about 2, a PUB/PEB ratio of greater than 0.36, and an emission maximum at 560 nm.
Claim 3
Dependent Claim
49 words
A phycoerythrin isolated from a biologically pure culture of marine cyanobacterium of the genus Synechococcus, said phycoerythrin characterized by absorption maxima at about 542, and at about 492, a 542/492 ratio of less than 2.0, a PUB/PEB ratio of greater than 0.36 and an emission maximum at 560 nm.
Claim 4
Dependent Claim
49 words
A phycoerythrin isolated from a biologically pure culture of marine cyanobacterium of the genus Synechococcus, said phycoerythrin characterized by absorption maxima at about 542, and at about 492, a 542/492 ratio of less than 2.0, a PUB/PEB ratio of greater than 0.36 and an emission maximum at 560 nm.
Claim 5
Dependent Claim
14 words
The phycoerythrin according to claim 2 wherein said cyanobacteria is Synechococcus WH8103 (ATCC 53061).
Claim 6
Dependent Claim
16 words
A phycoerythrin according to claim 2 wherein said 542/492 ratio is between 0 and about 1.9.
Claim 7
Dependent Claim
17 words
A phycoerythrin according to claim 4 wherein said 542/492 ratio is from about 0.4 to about 1.4.
Claim 8
Dependent Claim
13 words
A phycoerythrin according to claim 5 wherein said 542/492 ratio is about 0.4.
Claim 9
Dependent Claim
75 words
A fluorescent compound comprising a biliprotein isolated from a marine cyanobacterium of the genus Synechococcus, covalently conjugated to a member of a specific binding pair selected from the group consisting essentially of a ligand and a receptor, characterized in that the biliprotein essentially displays absorption maxima at about 542, and at about 492, a 542/492 ratio of less than about 2.0, a PUB/PEB ratio of greater than 0.36 and an emission maximum at 560 nm.
Claim 10
Dependent Claim
75 words
A fluorescent compound comprising a biliprotein isolated from a marine cyanobacterium of the genus Synechococcus, covalently conjugated to a member of a specific binding pair selected from the group consisting essentially of a ligand and a receptor, characterized in that the biliprotein essentially displays absorption maxima at about 542, and at about 492, a 542/492 ratio of less than about 2.0, a PUB/PEB ratio of greater than 0.36 and an emission maximum at 560 nm.
Claim 11
Dependent Claim
12 words
The compound according to claim 7 wherein said member is a receptor.
Claim 12
Dependent Claim
14 words
The compound according to claim 8 wherein said receptor is a cell surface antigen.
Claim 13
Dependent Claim
12 words
The compound according to claim 8 wherein said receptor is an antibody.
Claim 14
Dependent Claim
12 words
The compound according to claim 7 wherein said member is a ligand.
Claim 15
Dependent Claim
60 words
In a fluorescent activated cell sorting method employing a fluorescent biliprotein isolated from a marine cyanobacterium of the genus Synechococcus, the improvement which comprises employing a biliprotein having an absorption maxima at about 542 and at about 492, a 542/492 ratio of less than about 2, a PUB/PEB ratio of greater than 0.36 and an emission maximum at 560 nm.
Claim 16
Dependent Claim
60 words
In a fluorescent activated cell sorting method employing a fluorescent biliprotein isolated from a marine cyanobacterium of the genus Synechococcus, the improvement which comprises employing a biliprotein having an absorption maxima at about 542 and at about 492, a 542/492 ratio of less than about 2, a PUB/PEB ratio of greater than 0.36 and an emission maximum at 560 nm.
Claim 17
Dependent Claim
67 words
In a method of detecting cells or cellular components by means of fluorescent microscopy employing a fluorescent biliprotein isolated from a marine cyanobacterium of the genus Synechococcus, the improvement which comprises employing a biliprotein having absorption maxima at about 542 and at about 492, a 542/492 ratio of less than about 2, and a PUB/PEB ratio of greater than 0.36 and an emission maximum at 560 nm.
Claim 18
Dependent Claim
67 words
In a method of detecting cells or cellular components by means of fluorescent microscopy employing a fluorescent biliprotein isolated from a marine cyanobacterium of the genus Synechococcus, the improvement which comprises employing a biliprotein having absorption maxima at about 542 and at about 492, a 542/492 ratio of less than about 2, and a PUB/PEB ratio of greater than 0.36 and an emission maximum at 560 nm.
Claim 19
Dependent Claim
50 words
A biologically pure culture of marine cyanobacterium of the genus Synechococcus producing a phycoerythrin wherein said phycoerythrin has an absorption maxima at about 542 and about 492, a 542/492 ratio of less than about 2.0 a PUB/PEB ratio of greater than 0.36, and an emission maximum at about 560 nm.
Claim 20
Dependent Claim
50 words
A biologically pure culture of marine cyanobacterium of the genus Synechococcus producing a phycoerythrin wherein said phycoerythrin has an absorption maxima at about 542 and about 492, a 542/492 ratio of less than about 2.0 a PUB/PEB ratio of greater than 0.36, and an emission maximum at about 560 nm.
Claim 21
Dependent Claim
16 words
The cyanobacterium according to claim 14 wherein said cyanobacterium is a member of the genus Synechococcus.
Claim 22
Dependent Claim
12 words
The cyanobacterium according to claim 15 wherein said cyanobacterium is ATCC 53061.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-08-14 | 1993-02-13 | 1993-08-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-08-14 | 1997-02-13 | 1997-08-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-08-14 | 2001-02-13 | 2001-08-15 | $7700.00 | Window Closed / Lapsed |