Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
157 words
A method of obtaning a mammal characterized as having a plurality of cells containing exogenous genetic material, said material including at least one gene and a control sequence operably associated therewith, which, under predetermined conditions, express said gene under the control of said control sequence in a cell of said mammal, which comprises: (a) introducing exogenous genetic material into a pronucleus of a mammalian zygote by microinjection, said zygote being capable of development into a mammal, said genetic material including at least one gene and a control sequence operably associated therewith, thereby obtaining a genetically transformed zygote; (b) transplanting an embryo derived from the genetically transformed zygote into a pseudopregnant female capable of bearing the embryo to term; and (c) allowing the embryo to develop to term; where said gene and control sequence are selected so that the gene is not activated in such manner and degree as would prevent normal development of the embryo to term.
Claim 2
Independent Claim
157 words
A method of obtaning a mammal characterized as having a plurality of cells containing exogenous genetic material, said material including at least one gene and a control sequence operably associated therewith, which, under predetermined conditions, express said gene under the control of said control sequence in a cell of said mammal, which comprises: (a) introducing exogenous genetic material into a pronucleus of a mammalian zygote by microinjection, said zygote being capable of development into a mammal, said genetic material including at least one gene and a control sequence operably associated therewith, thereby obtaining a genetically transformed zygote; (b) transplanting an embryo derived from the genetically transformed zygote into a pseudopregnant female capable of bearing the embryo to term; and (c) allowing the embryo to develop to term; where said gene and control sequence are selected so that the gene is not activated in such manner and degree as would prevent normal development of the embryo to term.
Claim 3
Dependent Claim
16 words
The method of claim 1 wherein the control sequence is one naturally associated with the gene.
Claim 4
Dependent Claim
31 words
The method of claim 1 wherein the control sequence is one which will activate the gene when the cell is exposed to stimulus different from the natural stimulus of the gene.
Claim 5
Dependent Claim
22 words
The method of claim 1 wherein the control sequence is responsive to the same stimuli as is the beta globin control sequence.
Claim 6
Dependent Claim
33 words
The method of claim 1 in which at least about 1,000 copies of the gene are microinjected into the pronucleus and the volume of genetic material injected does not exceed about 10 picoliters.
Claim 7
Dependent Claim
25 words
The method of claim 1 in which the material is introduced into the male pronucleus of an egg having a separate female and male pronucleus.
Claim 8
Dependent Claim
24 words
The method of claim 1 in which the zygote is allowed to develop in vitro to the morula or blastocyst stage prior to transplantation.