US4877830A
Expired / Lapsed
US
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Metal chelate resins
Assignee: F Hoffmann La Roche AG
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Filing: 1987-07-13
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Granted: 1989-10-31
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Expires: 2007-07-13
Abstract
Abstract Metal chelate resins whose complexed nitrilotriacetic acid residues are bound to a carrier matrix via a spacer and which are suitable for metal chelate chromatography of proteins, especially those which contain neighboring histidines.
Inventors
- 👤 Heinz Dobeli
- 👤 Frich Hochuli
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 13:08
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K1/22; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01D15/3828; B01J; B01J20/00; B01J20/30; B01J20/32; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3265
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
14 words
A metal chelate resin of the formula: carrier matrix-spacer--NH--(CH.sub.2).sub.x --CH(COOH)--N(CH.sub.2 COO.sup.-).sub.2 Ni.sup.2+ wherein X=2-4.
Claim 2
Dependent Claim
14 words
A metal chelate resin of the formula: carrier matrix-spacer--NH--(CH.sub.2).sub.x --CH(COOH)--N(CH.sub.2 COO.sup.-).sub.2 Ni.sup.2+ wherein X=2-4.
Claim 3
Dependent Claim
14 words
The metal chelate resin of claim 1 wherein the carrier matrix is cross-linked agarose.
Claim 4
Dependent Claim
12 words
The metal chelate resin of claim 2 wherein the spacer is --O--CO--.
Claim 5
Independent Claim
48 words
A method for the manufacture of a metal chelate resin comprising the steps of: a) reacting a carrier matrix with a spacer; b) reacting the carrier matrix-spacer complex with a compound of the formula: H.sub.2 N--(CH.sub.2).sub.X --CH(COOH)--N(CH.sub.2 COOH).sub.2 wherein X=2-4; and c) washing with a nickel salt solution.
Claim 6
Independent Claim
48 words
A method for the manufacture of a metal chelate resin comprising the steps of: a) reacting a carrier matrix with a spacer; b) reacting the carrier matrix-spacer complex with a compound of the formula: H.sub.2 N--(CH.sub.2).sub.X --CH(COOH)--N(CH.sub.2 COOH).sub.2 wherein X=2-4; and c) washing with a nickel salt solution.
Claim 7
Dependent Claim
16 words
The method of claim 8, wherein the carrier matrix-spacer complex is oxirane-agarose which contains ##STR3## groups.
Claim 8
Dependent Claim
17 words
The method of claim 8 wherein the fuctionalized carrier matrix-spacer complex is imidazolecarbamate-agarose which contains ##STR4## groups.
References Cited (Backward Citations)
US4626416A
Cited
EP0253303A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-10-30 | 1993-05-01 | 1993-10-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-10-30 | 1997-05-01 | 1997-10-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-10-30 | 2001-05-01 | 2001-10-31 | $7700.00 | Window Closed / Lapsed |