US4886760A
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US
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Stable chemical compositions containing chromogenic materials, peroxides, and stabilizing chemicals
Assignee: Savyon Diagnostics Ltd
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Filing: 1986-07-31
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Granted: 1989-12-12
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Expires: 2006-07-31
Abstract
Abstract It is known that chromogenic materials which yield a color reaction when in the presence of a peroxide of the formula ROOR' which decomposes to yield ROR' and oxygen are unstable when in the form of working solutions for use in chromogenic reactions. Such compositions which also contain the peroxide required for such reactions are also unstable, leading to premature appearance of color. Stable chemical compositions are disclosed herein containing chromogenic materials and containing mixtures of chromogenic materials and peroxides, which can be used in chromogenic reactions after having been stored for long periods of time. Stabilizing chemical compositions and methods are also disclosed for obtaining such stable compositions.
Inventors
- 👤 Abraham Ben-Michael
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 11:39
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; Y; Y10; Y10S; Y10S436/00; Y10S436/904
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
77 words
A stable liquid composition containing a chromogen material and hydrogen peroxide, consisting essentially of an aqueous solvent medium, a chromogenic material, hydrogen peroxide, a water miscible organic solvent, a stabilizing unsaturated nitrogen heterocyclic compound and an effective amount of a chelating agent to stabilize said chromogen and hydrogen peroxide containing composition in its liquid state, said aqueous solvent medium being present in sufficient amount to provide a stable liquid composition containing at least 50% water by volume.
Claim 2
Dependent Claim
77 words
A stable liquid composition containing a chromogen material and hydrogen peroxide, consisting essentially of an aqueous solvent medium, a chromogenic material, hydrogen peroxide, a water miscible organic solvent, a stabilizing unsaturated nitrogen heterocyclic compound and an effective amount of a chelating agent to stabilize said chromogen and hydrogen peroxide containing composition in its liquid state, said aqueous solvent medium being present in sufficient amount to provide a stable liquid composition containing at least 50% water by volume.
Claim 3
Dependent Claim
91 words
The stable chromogen and hydrogen peroxide containing composition of claim 1, wherein said stabilizing compound is selected from the group consisting of compounds having the general formula: ##STR8## wherein each of Z and Q is either C or N, but both Z and Q are not N, each of R 16 , R 17 , and R 18 is independently selected from the group consisting of hydrogen, hydroxy, --NH, alkyl, amino, --SO 3 H, --CO 2 H, benzo, piperidino, pyrrolidino, substituted pyrrolidino, and an isoimidizole substituent; and salts of such compounds.
Claim 4
Dependent Claim
22 words
The stable chromogen and hydrogen peroxide containing composition of claims 1 or 2, having a pH of between about 2 and 5.
Claim 5
Dependent Claim
22 words
The stable chromogen and hydrogen peroxide containing composition of claims 1 or 2, having a pH of between about 7 and 11.
Claim 6
Dependent Claim
45 words
The stable chromogen and hydrogen peroxide containing composition of claim 2, wherein said stabilizing compound is selected from among the group consisting of 2-amino-4,6-diemthylpyridine, 2-amino-4,6-dimethylpyrimidine, 2-amino-6-methyl pyridine, 2,4,6-collidine, 3-(aminomethyl) pyridine, 2-quinoxalinol, 4-amino-2,6-dimethylpyridine, 5-ethyl-2-methylpyridine, 4-methylpyrimidine, 2,6-lutidin, ethyl nicotinate, 4-piperidinopyridine, 2-aminoethylpyridine, 4-pyridinecarboxylic acid, pyridine-3-sulfonic acid and purine.
Claim 7
Dependent Claim
37 words
The stable chromogen and hydrogen peroxide containing composition of claim 2, wherein said aqueous solvent medium contains a buffer solution selected from the group consisting of phosphate buffer, Tris buffer(hydroxymethyl-aminomethane), borate buffer, glycine buffer and acetate buffer.
Claim 8
Dependent Claim
28 words
The stable chromogen and hydrogen peroxide containing composition of claim 1, wherein said water miscible organic solvent has a gas-phase dipole moment of at least about 1.60 D.
Claim 9
Dependent Claim
28 words
The stable chromogen and hydrogen peroxide containing composition of claim 7, wherein said water miscible organic solvent has a gas-phase dipole moment of at least about 1.69 D.
Claim 10
Dependent Claim
21 words
The stable chromogen and hydrogen peroxide containing composition of claim 1, wherein said water miscible organic solvent contains an aprotic solvent.
Claim 11
Dependent Claim
53 words
The stable chromogen and hydrogen peroxide containing composition of claim 1, wherein said chromogenic material is selected from the group consisting of chloronaphthol, benzidine, tetramethylbenzidine, 3-amino-9-ethylcarbazole, dichloronaphthol, dibromonaphthol, 3,3',5,5'-tetramethylbenzidine dihydrochloride, 3,3',5,5'-tetramethylbenzidine dihydrate, 3,3',5,5'-tetramethylbenzidine dihydrochloride dihydrate, 3-methylbenzothiazole-2,1-hydrazone, parahydroxyphenyl acetic acid, 2,2'-azino-di (3-ethylbenzothiazoline) sulfonic acid, 4-aminoantipyrin/alpha-naphthol, o-dianizidine, o-toluidine, 5-amino salicilic acid, guaiacol, o-tolidine and pyrogallol.
Claim 12
Dependent Claim
20 words
The stable chromogen and hydrogen peroxide containing composition of claim 1, wherein said aqueous solvent medium contains a buffer solution.
Claim 13
Dependent Claim
34 words
The stable chromogen and hydrogen peroxide containing composition of claim 1, wherein said chelating agent is selected from the group consisting of pyrophosphate, nitrilotriacetic acid, acetanilide, citrate, a derivative of 8-hydroxyquinoline and mixtures thereof.
Claim 14
Dependent Claim
53 words
The stable chromogen and hydrogen peroxide containing composition of claim 12, wherein said chelating agent contains up to about 200 ppm of said pyrophosphate, up to about 200 ppm of said acetanilide, up to about 1000 ppm of said citrate, and up to about 50 ppm of said 8-hydroxyquinoline or a salt thereof.
Claim 15
Dependent Claim
42 words
The stable chromogen and hydrogen peroxide containing composition of claim 1, wherein said water miscible organic solvent is selected from the group consisting of methanol, ethanol, dioxane, dimethylsulfoxide, sulfolane, acetamide, soluted dimethylsulfone, hexamethylphosphoric triamide, N-methylacetamide, dioxane, dimethylformamide, soluted trioxane, formamide and tetrahydrofuran.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-12-11 | 1993-06-12 | 1993-12-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-12-11 | 1997-06-12 | 1997-12-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-12-11 | 2001-06-12 | 2001-12-12 | $7700.00 | Window Closed / Lapsed |