US4895704A
Expired / Lapsed
US
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Integral multilayer analytical element
Assignee: Fujifilm Holdings Corp
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Filing: 1987-10-27
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Granted: 1990-01-23
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Expires: 2007-10-27
Abstract
Abstract An integral multilayer analytical element which comprises a water-impermeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder and a spreading layer superposed in this order, and contains at least one reagent capable of reacting with a component of a sample to produce a detectable species capable of detecting by light, which is characterized in that light-scattering particulates in an amount to make light transmittance to 10 to 2.5% are contained in the above hydrophilic layer and/or a layer located on the side of the support therefrom. This element may decrease the influence of a conventional reagent layer up to the extent being negligible without lowering the sensitivity. Precision of the measurement at a low concentration range is also high, and reproducibility of the meaurement is well. Manufacture of this element is easy.
Inventors
- 👤 Fuminori Arai
- 👤 Takeshi Igarashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 14:00
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
78 words
In an integral multilayer analytical element which comprises a water-impermeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder and a spreading layer superposed in this order, and containing at least one reagent capable of reacting with a component of a sample to produce a detectable species capable of detection by light, the improvement which comprises said hydrophilic layer containing light-scattering particulates in an amount to make light transmittance in the range from 10 to 2.5%.
Claim 2
Dependent Claim
78 words
In an integral multilayer analytical element which comprises a water-impermeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder and a spreading layer superposed in this order, and containing at least one reagent capable of reacting with a component of a sample to produce a detectable species capable of detection by light, the improvement which comprises said hydrophilic layer containing light-scattering particulates in an amount to make light transmittance in the range from 10 to 2.5%.
Claim 3
Dependent Claim
92 words
In an integral multilayer analytical element which comprises a water-impermeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder and a spreading layer superposed in this order, and containing at least one reagent capable of reacting with a component of a sample to produce a detectable species capable of detection by light, the improvement which comprises an additional layer on the same side of the support as said hydrophilic layer, said additional layer containing light-scattering particulates in an amount to make light transmittance in the range from 10 to 2.5%.
Claim 4
Dependent Claim
92 words
In an integral multilayer analytical element which comprises a water-impermeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder and a spreading layer superposed in this order, and containing at least one reagent capable of reacting with a component of a sample to produce a detectable species capable of detection by light, the improvement which comprises an additional layer on the same side of the support as said hydrophilic layer, said additional layer containing light-scattering particulates in an amount to make light transmittance in the range from 10 to 2.5%.
Claim 5
Dependent Claim
17 words
The integral multilayer analytical element of claim 1, wherein said reagent is contained in said spreading layer.
Claim 6
Dependent Claim
28 words
The integral multilayer analytical element of claim 1 or 2, wherein mean particle size of said light-scattering particulates is in the range of 0.1 μm to 2 μm.
Claim 7
Dependent Claim
20 words
The integral multilayer analytical element of claim 4, wherein said light-scattering particulates are titanium dioxide particulates or barium sulfate particulates.
Claim 8
Dependent Claim
20 words
The integral multilayer analytical element of claim 1 or 2, wherein said light-scattering particulates are contained in said hydrophilic layer.
Claim 9
Dependent Claim
19 words
The integral multilayer analytical element of claim 4 or 6, wherein said reagent is contained in said hydrophilic layer.
Claim 10
Dependent Claim
17 words
The integral multilayer analytical element of claim 7 wherein a mordant is contained in said hydrophilic layer.
Claim 11
Dependent Claim
28 words
The integral multilayer analytical element of claim 1 or 2, wherein said support is selected from the group consisting of polyethylene terephthalate, polycarbonate, a cellulose ester and polystyrene.
Claim 12
Dependent Claim
35 words
The integral multilayer analytical element of claim 1 or 2, wherein said hydrophilic polymer binder is selected from the group consisting of gelatin, gelatin derivatives, agarose, pullulan, pullulan derivatives, polyacrylamide, polyvinyl alcohol and polyvinyl pyrrolidone.
Claim 13
Dependent Claim
56 words
The integral multilayer analytical element of claim 1 or 2, wherein said spreading layer is selected from the group consisting of a membrane filter, a continuous microspaces-containing porous layer, a spreading layer of woven fabric, a spreading layer of knitted fabric and a spreading layer comprising a paper containing a fibrous pulp of an organic polymer.
Claim 14
Dependent Claim
18 words
The integral multilayer analytical element of claim 1 or 2, wherein said hydrophilic layer contains a water-absorption layer.
Claim 15
Dependent Claim
16 words
The analytical element of claim 1 or 2 wherein the hydrophilic layer contains a registration layer.
Claim 16
Dependent Claim
104 words
In a method for the detection of a blood component wherein a blood sample is placed on an integral multilayer analytical element and analyzed the improvement wherein said analytical element comprises a superposed in this order, a water-permeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder, and a spreading layer, and which further contains at least one reagent capable of reacting with a component of the sample to produce a detectable species capable of detection by light, and wherein said hydrophilic layer contains light scattering particulates in an amount to make the light transmittance in the range from 10 to 2.5%.
Claim 17
Dependent Claim
104 words
In a method for the detection of a blood component wherein a blood sample is placed on an integral multilayer analytical element and analyzed the improvement wherein said analytical element comprises a superposed in this order, a water-permeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder, and a spreading layer, and which further contains at least one reagent capable of reacting with a component of the sample to produce a detectable species capable of detection by light, and wherein said hydrophilic layer contains light scattering particulates in an amount to make the light transmittance in the range from 10 to 2.5%.
Claim 18
Dependent Claim
18 words
The method of claim 14, wherein said component is cholesterol, uric acid, creatinine, enzymes, ammonia, saccharides or proteins.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-01-22 | 1993-07-24 | 1994-01-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-01-22 | 1997-07-24 | 1998-01-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-01-22 | 2001-07-24 | 2002-01-23 | $7700.00 | Window Closed / Lapsed |