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US4897306A Expired / Lapsed US ⏱️ 0.0 years remaining

Medium for electrophoresis

Assignee: Fujifilm Holdings Corp
•
Filing: 1987-04-20
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Granted: 1990-01-30
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Expires: 2007-04-20
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Abstract

Abstract An improvement of a medium for electrophoresis which comprises a support of a plastic material and an aqueous polyacrylamide gel, wherein a resin layer comprises a resin having an oxygen permeability coefficient lower than that of the plastic material of the support is arranged betwen the support and the polyacrylamide gel. The resin preferably shows an oxygen permeability of not more than 15 cc/m 2 ·atm·day in the form of a resinous layer of 20 μm thick, and exemplified by a vinylidene chloride-vinyl chloride copolymer, polyvinyl alcohol, polyacrylonitrile, polyvinylidene chloride, a vinyl alcohol-ethylene copolymer, a vinylidene chloride-acrylonitrile copolymer, and an acrylonitrile-methyl acrylate-butadiene copolymer.

Inventors

  • 👤 Naohiko Sugimoto
  • 👤 Daijiro Nishio
  • 👤 Elichi Hasegawa

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-29 03:26
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; B; B01; B01D; B01D57/00; B01D57/02; Y; Y10; Y10T; Y10T428/00; Y10T428/26; Y10T428/263; Y10T428/264; Y10T428/265; Y10T428/269; Y10T428/31504; Y10T428/31551; Y10T428/31645; Y10T428/31649; Y10T428/31786; Y10T428/31797; Y10T428/31855; Y10T428/3188; Y10T428/31884; Y10T428/31891; Y10T428/31909; Y10T428/31913; Y10T428/3192; Y10T428/31928

Claims Analysis

Total Claims: 10
Claim 1
Independent Claim 126 words
In a medium for electrophoresis which comprises a support of a plastic material selected from the group consisting of polyethylene terephthalate, the polycarbonate of Bisphenol A, polyvinyl chloride, vinylidene chloride-vinyl chloride copolymer, polymethyl methacrylate, polyethylene, polypropylene, cellulose acetates, and cellulose acetate propionate, and an aqueous polyacrylamide gel membrane formed by the crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, the improvement which comprises a resin layer between the support and the aqueous polyacrylamide gel membrane comprising a resin having an oxygen permeability coefficient lower than that of the plastic material of the support, said coefficient being not more than 15 cc/m 2 ·atm·day when the resin is in the form of a film having a thickness of 20 μm.
Claim 2
Dependent Claim 126 words
In a medium for electrophoresis which comprises a support of a plastic material selected from the group consisting of polyethylene terephthalate, the polycarbonate of Bisphenol A, polyvinyl chloride, vinylidene chloride-vinyl chloride copolymer, polymethyl methacrylate, polyethylene, polypropylene, cellulose acetates, and cellulose acetate propionate, and an aqueous polyacrylamide gel membrane formed by the crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, the improvement which comprises a resin layer between the support and the aqueous polyacrylamide gel membrane comprising a resin having an oxygen permeability coefficient lower than that of the plastic material of the support, said coefficient being not more than 15 cc/m 2 ·atm·day when the resin is in the form of a film having a thickness of 20 μm.
Claim 3
Dependent Claim 24 words
The medium for electrophoresis as claimed in claim 1, wherein the resin layer has a thickness in the range of 0.5 to 20 μm.
Claim 4
Dependent Claim 16 words
The medium for electrophoresis as claimed in claim 1 wherein the plastic material is polyethylene terephthalate.
Claim 5
Dependent Claim 47 words
The medium for electrophoresis as claimed in claim 1, wherein the resin layer comprises at least one resin selected from the group consisting of a vinylidene chloride-vinyl chloride copolymer, polyvinyl alcohol, polyacrylonitrile, polyvinylidene chloride, a vinyl alcohol-ethylene copolymer, a vinylidene chlorideacrylonitrile copolymer, and an acrylonitrile-methyl acrylate-butadiene copolymer.
Claim 6
Dependent Claim 121 words
In a medium for electrophoresis which comprises a support of a plastic material selected from the group consisting of polyethylene terephthalate, the polycarbonate of Bisphenol A, polyvinyl chloride, vinylidene chloride-vinyl chloride copolymer, polymethyl methacrylate, polyethylene, polypropylene, cellulose acetates, and cellulose acetate propionate, and an aqueous polyacrylamide gel membrane formed by the crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, the improvement which comprises a resin layer between the support and the aqueous polyacrylamide gel membrane comprising a resin having an oxygen permeability coefficient lower than that of the plastic material of the support, a composite of the support and the resin layer exhibiting an oxygen permeability of not more than 10 cc/m 2 ·atom·day.
Claim 7
Dependent Claim 121 words
In a medium for electrophoresis which comprises a support of a plastic material selected from the group consisting of polyethylene terephthalate, the polycarbonate of Bisphenol A, polyvinyl chloride, vinylidene chloride-vinyl chloride copolymer, polymethyl methacrylate, polyethylene, polypropylene, cellulose acetates, and cellulose acetate propionate, and an aqueous polyacrylamide gel membrane formed by the crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, the improvement which comprises a resin layer between the support and the aqueous polyacrylamide gel membrane comprising a resin having an oxygen permeability coefficient lower than that of the plastic material of the support, a composite of the support and the resin layer exhibiting an oxygen permeability of not more than 10 cc/m 2 ·atom·day.
Claim 8
Dependent Claim 24 words
The medium for electrophoresis as claimed in claim 5 wherein the resin layer has a thickness in the range of 0.5 to 20 μm.
Claim 9
Dependent Claim 51 words
The medium for electrophoresis as claimed in claim 5 wherein the resin layer comprises at least one resin selected from the group consisting of a vinylidene chloridevinyl chloride copolymer, polyvinyl alcohol, polyacrylonitrile, polyvinylidene chloride, a vinyl alcohol-ethylene copolymer, a vinylidene chloride-acrylonitrile copolymer, a vinylidene chloride-acrylonitrile copolymer, and an acrylonitrile-methyl acrylate-butadiene copolymer.
Claim 10
Dependent Claim 16 words
The medium for electrophoresis as claimed in claim 5 wherein the plastic material is polyethylene terephthalate.

References Cited (Backward Citations)

US4048377A Cited
US4383376A Cited
US4548870A Cited
US4548869A Cited
US4631235A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1993-01-29 1993-07-31 1994-01-30 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1997-01-29 1997-07-31 1998-01-30 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2001-01-29 2001-07-31 2002-01-30 $7700.00 Window Closed / Lapsed

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