US4898813A
Expired / Lapsed
US
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Catalytic test composition intended to produce a range of colors
Assignee: Bayer Corp
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Filing: 1986-04-04
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Granted: 1990-02-06
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Expires: 2006-04-04
Abstract
Abstract Test compositions and test devices are provided which are capable of generating different hues at different analyte concentrations. Visual results for clinically important analytes, such as glucose and cholesterol, are provided by use of compositions containing two independent catalytic systems which are reactive with a common substrate generated from the analyte of interest, to produce visual endpoints of different hues for different concentrations of analyte. Preferred formulations provide a RAINBOW of hues, the particular final hue produced depending on the concentration of the analyte.
Inventors
- 👤 James P. Albarella
- 👤 Steven C. Charlton
- 👤 James W. Reinsch
- 👤 Mary E. Warchal
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 01:12
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/80; Y10S436/805
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
121 words
Process for determining the concentration of an analyte in a fluid sample, which process comprises: contacting a common substrate generating system capable of generating a common substrate by reaction with an analyte of interest, with (a) an analyte, (b) a first catalytic system capable of generating a change in hue of a first indicator component by reaction with the common substrate, and (c) a second catalytic system capable of generating a change in hue of a second indicator component by reaction with the common substrate, wherein the generation of the change in hues of the first and second indicator components occurs substantially simultaneously and provides different hues at different analyte concentrations, the final hue depending on the concentration of the analyte.
Claim 2
Dependent Claim
121 words
Process for determining the concentration of an analyte in a fluid sample, which process comprises: contacting a common substrate generating system capable of generating a common substrate by reaction with an analyte of interest, with (a) an analyte, (b) a first catalytic system capable of generating a change in hue of a first indicator component by reaction with the common substrate, and (c) a second catalytic system capable of generating a change in hue of a second indicator component by reaction with the common substrate, wherein the generation of the change in hues of the first and second indicator components occurs substantially simultaneously and provides different hues at different analyte concentrations, the final hue depending on the concentration of the analyte.
Claim 3
Dependent Claim
24 words
The process of claim 1 in which a color retardant is added to delay the reaction of the common substrate with a catalytic systems.
Claim 4
Dependent Claim
14 words
The test composition of claim 2 in which the color retardant is potassium ferrocyanide.
Claim 5
Dependent Claim
146 words
The test composition for the determination of the concentration of an analyte in a fluid sample, comprising: (a) a common substrate generating system which in the presence of an analyte in a fluid sample will generate a common substrate selected from the group consisting of adenosine triphosphate, reduced flavin adenine dinucleotide, hydrogen peroxide, glycerol and glucose; (b) a first catalytic system capable of generating a change in hue in a first indicator upon reaction with the common substrate; and (c) a second catalytic system capable of generating a change in hue in a second indicator by reaction with the common substrate, wherein the generation of the change in hues of the first and second indicators occurs substantially simultaneously and provides different hues at different analyte concentrations, the final hue produced by the test composition depending on the concentration of the analyte present in the fluid sample.
Claim 6
Dependent Claim
146 words
The test composition for the determination of the concentration of an analyte in a fluid sample, comprising: (a) a common substrate generating system which in the presence of an analyte in a fluid sample will generate a common substrate selected from the group consisting of adenosine triphosphate, reduced flavin adenine dinucleotide, hydrogen peroxide, glycerol and glucose; (b) a first catalytic system capable of generating a change in hue in a first indicator upon reaction with the common substrate; and (c) a second catalytic system capable of generating a change in hue in a second indicator by reaction with the common substrate, wherein the generation of the change in hues of the first and second indicators occurs substantially simultaneously and provides different hues at different analyte concentrations, the final hue produced by the test composition depending on the concentration of the analyte present in the fluid sample.
Claim 7
Dependent Claim
21 words
The test composition of claim 4 in which the common substrate generating system is glucose oxidase and the analyte is glucose.
Claim 8
Dependent Claim
21 words
The test composition of claim 4 in which the common substrate generating system is cholesterol oxidase and the analyte is cholesterol.
Claim 9
Dependent Claim
21 words
The test composition of claim 4 in which the common substrate generating system is alcohol oxidase and the analyte is alcohol.
Claim 10
Dependent Claim
21 words
The test composition of claim 4 in which the common substrate generating system is lipoprotein lipase and the analyte is triglyceride.
Claim 11
Dependent Claim
22 words
The test composition of claim 8 in which the first catalytic system comprises glycerolkinase and the second catalytic system comprises glycerol dehydrogenase.
Claim 12
Dependent Claim
22 words
The test composition of claim 4 in which the common substrate generating system is alpha amylase and the analyte is alpha amylase.
Claim 13
Dependent Claim
22 words
The test composition of claim 10 in which the first catalytic system comprises hexokinase and the second catalytic system comprises glucose dehydrogenase.
Claim 14
Dependent Claim
27 words
The test composition of claim 4 in which the change in hue of one of the first or second indicators is from a hue to substantially colorless.
Claim 15
Dependent Claim
29 words
The test composition of claim 4 in which a color retardant is added to the test composition to delay the reaction of the common substrate with the catalytic systems.
Claim 16
Dependent Claim
24 words
The test composition of claim 4 which also contains a buffer for maintaining the pH of the test composition between about 6 and 9.
Claim 17
Dependent Claim
161 words
The test device for the determination of concentration of an analyte in a fluid sample comprising: a carrier matrix, and a test composition incorporated into the carrier matrix, said test composition comprising: (a) a common substrate generating system which in the presence of an analyte in a fluid sample will generate a common substrate selected from the group consisting of adenosine triphosphate, reduced flavin adenine dinucleotide, hydrogen peroxide, glycerol and glucose; (b) a first catalytic system capable of generating a change in hue in a first indicator upon reaction with the common substrate; and (c) a second catalytic system capable of generating a change in hue in a second indicator by reaction with the common substrate, wherein the generation of the change in hues of the first and second indicators occurs substantially simultaneously and provides different hues at different analyte concentrations, the final hue produced by the test composition depending on the concentration of the analyte present in the fluid sample.
Claim 18
Dependent Claim
161 words
The test device for the determination of concentration of an analyte in a fluid sample comprising: a carrier matrix, and a test composition incorporated into the carrier matrix, said test composition comprising: (a) a common substrate generating system which in the presence of an analyte in a fluid sample will generate a common substrate selected from the group consisting of adenosine triphosphate, reduced flavin adenine dinucleotide, hydrogen peroxide, glycerol and glucose; (b) a first catalytic system capable of generating a change in hue in a first indicator upon reaction with the common substrate; and (c) a second catalytic system capable of generating a change in hue in a second indicator by reaction with the common substrate, wherein the generation of the change in hues of the first and second indicators occurs substantially simultaneously and provides different hues at different analyte concentrations, the final hue produced by the test composition depending on the concentration of the analyte present in the fluid sample.
Claim 19
Dependent Claim
19 words
The test device of claim 15 in which at least one indicator component is compartmentalized within the carrier matrix.
Claim 20
Dependent Claim
23 words
The test device of claim 16 in which at least one indicator component is compartmentalized within a gelatin layer within a carrier matrix.
Claim 21
Dependent Claim
13 words
The test device of claim 15 in which the carrier matrix is paper.
Claim 22
Dependent Claim
15 words
The test device of claim 15 in which the carrier matrix is a hydrophilic carrier.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-02-05 | 1993-08-07 | 1994-02-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-02-05 | 1997-08-07 | 1998-02-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-02-05 | 2001-08-07 | 2002-02-06 | $7700.00 | Window Closed / Lapsed |