US4909920A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Automatic electrophoresis apparatus and method
Assignee: Helena Laboratories Corp
•
Filing: 1988-09-12
•
Granted: 1990-03-20
•
Expires: 2008-09-12
Abstract
Abstract An electrophoresis machine is disclosed in which after a microporous support strip and a sample plate are placed in the machine, all electrophoresis processing, scanning and densitometer functions are automatically performed under computer control. The machine includes apparatus for automatic pipetting of liquid samples from the sample plate to the surface of the support strip, apparatus for applying electrophoresis current to the strip while simultaneously cooling it, apparatus for applying and spreading flourescent staining reagent to the strip, and apparatus for incubating the strip and subsequently drying it. A T.V. camera, mounted to a flourescent scanning box enclosing the application plate, in cooperation with digital processing equipment, electronically scans electrophoresically longitudinally displaced components of the samples for determining relative component densities. An alternative scanning apparatus including a collimator and photomultiplier is also disclosed.
Inventors
- 👤 Robert J. Sarrine
- 👤 Henry A. Garsee
- 👤 Charles D. Kelley
- 👤 Philip A. Guadagno
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 15:40
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721; G01N27/44756; G01N27/44782
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
137 words
A method of determining relative components of a liquid sample in an apparatus having an application plate disposed on a base and an electrophoresis support medium removably secured to a top surface of said application plate, and a sample plate having at least two liquid sample wells having liquid to be sampled disposed therein, where the sample plate is separated from the application plate, the method comprising the steps of, robotically applying simultaneously at least two liquid samples from said at least two liquid sample wells of said sample plate to said support medium while said support medium is disposed on said application plate, and while continuously maintaining the position of said support medium on said application plate, applying electrophoresis current to said support medium, operably creating an electrophoretically displaced pattern of components of said liquid samples.
Claim 2
Independent Claim
137 words
A method of determining relative components of a liquid sample in an apparatus having an application plate disposed on a base and an electrophoresis support medium removably secured to a top surface of said application plate, and a sample plate having at least two liquid sample wells having liquid to be sampled disposed therein, where the sample plate is separated from the application plate, the method comprising the steps of, robotically applying simultaneously at least two liquid samples from said at least two liquid sample wells of said sample plate to said support medium while said support medium is disposed on said application plate, and while continuously maintaining the position of said support medium on said application plate, applying electrophoresis current to said support medium, operably creating an electrophoretically displaced pattern of components of said liquid samples.
Claim 3
Independent Claim
116 words
A method of determining relative components of a liquid sample in an apparatus having an application plate longitudinally disposed on a base and an electrophoresis support medium removably secured to a top surface of said application plate, the method comprising the steps of, applying a liquid sample to said support medium while said support medium is disposed on said application plate, while continuously maintaining the position of said support medium on said application plate, longitudinally applying electrophoresis current to said support medium, operably creating an electrophoretically longitudinally displaced pattern of components of said liquid sample, and while continuously maintaining the position of said support medium on said application plate, staining, incubating and drying said support medium.
Claim 4
Independent Claim
116 words
A method of determining relative components of a liquid sample in an apparatus having an application plate longitudinally disposed on a base and an electrophoresis support medium removably secured to a top surface of said application plate, the method comprising the steps of, applying a liquid sample to said support medium while said support medium is disposed on said application plate, while continuously maintaining the position of said support medium on said application plate, longitudinally applying electrophoresis current to said support medium, operably creating an electrophoretically longitudinally displaced pattern of components of said liquid sample, and while continuously maintaining the position of said support medium on said application plate, staining, incubating and drying said support medium.
Claim 5
Dependent Claim
35 words
The method of claim 2 further comprising the step of, while continuously maintaining the position of said support medium on said application plate, generating an electronic image of the top surface of said support medium.
Claim 6
Dependent Claim
130 words
Apparatus for determining relative components of a liquid sample comprising a base, an application plate longitudinally disposed on said base, an electrophoresis support medium removably secured in a position on a top surface of said application plate, a sample plate disposed on said base having at least two liquid sample wells having liquid to be sampled disposed therein, where said sample plate is separated from said application plate, robotic means for simultaneously applying at least two liquid samples from said at least two liquid sample wells of said sample plate to said support medium while said support medium is in said position on said application plate, and means for applying electrophoresis current to said support medium while said support medium is continuously maintained in said position on said application plate.
Claim 7
Dependent Claim
130 words
Apparatus for determining relative components of a liquid sample comprising a base, an application plate longitudinally disposed on said base, an electrophoresis support medium removably secured in a position on a top surface of said application plate, a sample plate disposed on said base having at least two liquid sample wells having liquid to be sampled disposed therein, where said sample plate is separated from said application plate, robotic means for simultaneously applying at least two liquid samples from said at least two liquid sample wells of said sample plate to said support medium while said support medium is in said position on said application plate, and means for applying electrophoresis current to said support medium while said support medium is continuously maintained in said position on said application plate.
Claim 8
Dependent Claim
107 words
Apparatus for determining relative components of a liquid sample comprising a base, an application plate longitudinally disposed on said base, an electrophoresis support medium removably secured in a position on a top surface of said application plate, means for applying a liquid sample to said support medium while said support medium is in said position on said application plate, means for longitudinally applying electrophoresis current to said support medium while said support medium is continuously maintained in said position on said application plate, and means for staining, incubating and drying said support medium while said support medium is continuously maintained in said position on said application plate.
Claim 9
Dependent Claim
107 words
Apparatus for determining relative components of a liquid sample comprising a base, an application plate longitudinally disposed on said base, an electrophoresis support medium removably secured in a position on a top surface of said application plate, means for applying a liquid sample to said support medium while said support medium is in said position on said application plate, means for longitudinally applying electrophoresis current to said support medium while said support medium is continuously maintained in said position on said application plate, and means for staining, incubating and drying said support medium while said support medium is continuously maintained in said position on said application plate.
Claim 10
Dependent Claim
34 words
The apparatus of claim 3 further comprising means for generating an electronic image of the top surface of said support medium while continuously maintaining said position of said support medium on said application plate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-03-19 | 1993-09-18 | 1994-03-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-03-19 | 1997-09-18 | 1998-03-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-03-19 | 2001-09-18 | 2002-03-20 | $7700.00 | Window Closed / Lapsed |