US4921791A
Expired / Lapsed
US
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Method for measuring specific component using peroxidase enzyme reaction
Assignee: Konica Minolta Inc
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Filing: 1987-06-25
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Granted: 1990-05-01
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Expires: 2007-06-25
Abstract
Abstract A method for measuring a specific component, which comprises having a complex conjugate comprising a specific component to be measured and a labelled body having peroxidase as the label carried on a support, and forming and depositing a dye on said complex conjugate through the enzyme reaction of peroxidase by use of the three of hydrogen peroxide, an aromatic primary amine compound and either one of an active methylene compound or a phenol compound.
Inventors
- 👤 Masahiko Yamasaki
- 👤 Satoshi Kawakatsu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 16:12
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/581; C12Q2326/00; C12Q2326/50
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
161 words
A method for measuring a specific component, said method comprising the steps of forming and providing a complex conjugate comprising a specific component to be measured and a peroxidase labelled ligand carried on a support; and forming and depositing a water-insoluble dye on said complex conjugate through the enzyme reaction or peroxidase with hydrogen peroxide, a p-phenylenediamine type aromatic primary amine compound and either one of an active methylene compound or a phenol compound, wherein said aromatic primary amine compound is represented by the formula: ##STR6## wherein A and B each represents a hydrogen atom, an alkyl group, or A and B may form a heterocyclic ring together with a nitrogen atom; D, E, G and J each represents a substituent selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, an amino group, an alkoxy group, an acylamide group, an aryl-sulfonamide group, an alkylsulfonamide group and an alkyl group, or a salt of said compound.
Claim 2
Independent Claim
161 words
A method for measuring a specific component, said method comprising the steps of forming and providing a complex conjugate comprising a specific component to be measured and a peroxidase labelled ligand carried on a support; and forming and depositing a water-insoluble dye on said complex conjugate through the enzyme reaction or peroxidase with hydrogen peroxide, a p-phenylenediamine type aromatic primary amine compound and either one of an active methylene compound or a phenol compound, wherein said aromatic primary amine compound is represented by the formula: ##STR6## wherein A and B each represents a hydrogen atom, an alkyl group, or A and B may form a heterocyclic ring together with a nitrogen atom; D, E, G and J each represents a substituent selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, an amino group, an alkoxy group, an acylamide group, an aryl-sulfonamide group, an alkylsulfonamide group and an alkyl group, or a salt of said compound.
Claim 3
Dependent Claim
35 words
A method for measuring a specific component according to claim 1, wherein said specific component is directly or indirectly carried on the support before the reaction with said labelled ligand to form a complex conjugate.
Claim 4
Dependent Claim
29 words
A method for measuring a specific component according to claim 1, wherein said complex conjugate is formed before said complex conjugate is directly or indirectly carried on the support.
Claim 5
Dependent Claim
50 words
A method for measuring a specific component according to claim 1, wherein said active methylene compound is selected from the group consisting of an acylacetonitrile derivative, an acylacetamide derivative, a 1,3-diketone derivative, a 5-pyrazolone derivative, a pyrazolol(2,3-a)benzimidazole derivative, a pyrazolo-(3,2-c)-5-triazole derivative, a cyanoacetyl substituted heterocyclic compound and an indazolone derivative.
Claim 6
Dependent Claim
65 words
A method for measuring a specific component according to claim 1, wherein said phenol compound is represented by the formula: ##STR7## wherein R 1 represents a monovalent organic group or atom, Z represents a hydrogen atom or a group or atom eliminable during the coupling reaction with the oxidized product of said aromatic primary amine compound, and k represents an integer of 1 to 4.
Claim 7
Dependent Claim
30 words
A method for measuring a specific component according to claim 5, wherein said phenol compound has a phenyl ring bonded between R 1 substituents at the 5- and 6- positions.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-04-30 | 1993-10-30 | 1994-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-04-30 | 1997-10-30 | 1998-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-04-30 | 2001-10-30 | 2002-05-01 | $7700.00 | Window Closed / Lapsed |