US4927769A
Expired / Lapsed
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Method for enhancement of chemiluminescence
Assignee: Bayer Corp
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Filing: 1987-07-08
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Granted: 1990-05-22
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Expires: 2007-07-08
Abstract
Abstract A method for enhancing the chemiluminescent signal of an acridinium ester in a chemiluminescent reaction which comprises oxidizing the acridinium ester in the presence of an enhancer selected from the group consisting of: (a) a cationic surfactant; (b) a nonionic surfactant; and (c) a sulfated primary alcohol.
Inventors
- 👤 Steve C. S. Chang
- 👤 Thomas E. Miller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 02:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; G01N33/52; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/826; Y10S530/00; Y10S530/802
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
102 words
A method for enhancing the chemiluminescent signal of an acridinium ester in a chemiluminescent reaction which comprises oxidizing the acridinium ester in the presence of an effective amount of an enhancer to effect enhanced chemiluminescent signal said enhancer selected from the group consisting of: (a) a quarternary amine cationic surfactant of formula: ##STR5## wherein R 1 is C 8 -C 20 alkyl or alkenyl; and R 2 R 3 and R 4 are C 1 -C 4 alkyl or alkenyl. (b) a nonionic surfactant; and (c) a sulfated primary alcohol selected from the group consisting of sodium dodecylsulfate, magnesium dodecylsulfate and tridecylsulfate.
Claim 2
Independent Claim
102 words
A method for enhancing the chemiluminescent signal of an acridinium ester in a chemiluminescent reaction which comprises oxidizing the acridinium ester in the presence of an effective amount of an enhancer to effect enhanced chemiluminescent signal said enhancer selected from the group consisting of: (a) a quarternary amine cationic surfactant of formula: ##STR5## wherein R 1 is C 8 -C 20 alkyl or alkenyl; and R 2 R 3 and R 4 are C 1 -C 4 alkyl or alkenyl. (b) a nonionic surfactant; and (c) a sulfated primary alcohol selected from the group consisting of sodium dodecylsulfate, magnesium dodecylsulfate and tridecylsulfate.
Claim 3
Dependent Claim
144 words
A method as recited in claim 1 wherein the acridinium ester has the formula: ##STR6## where R 1 is alkyl, alkenyl, alkynyl, or aryl; R 2 , R 3 , R 5 , or R 7 are hydrogen, amino, carboxyl, hydroxyl, alkoxyl, nitro, or halide; R 4 or R 8 are hydrogen, alkyl, alkenyl, alkynyl, aryl, alkoxyl, or amino,; R 6 represents the following substituent: R.sub.6 =--R.sub.9 --R.sub.10 where R 9 is omitted or is alkyl, aryl, or aralkyl linking groups, and R 10 is selected from the group consisting of: ##STR7## X is CH 3 SO4, OSO 2 F, halide, OSO 2 CF 3 , OSO 2 C 4 F 9 , or ##STR8## and R is alkyl, aryl, or alralkyl; and finally R 5 , and R 7 substituent positions on the phenoxy ring are interchangeable with the R 6 substituent position.
Claim 4
Dependent Claim
21 words
A method as recited in claim 2 werein R 4 and R 8 are alkyl, alkenyl, alkynyl, aryl, alkoxyl, or amino.
Claim 5
Dependent Claim
56 words
A method as recited in claim 2 wherein R 1 is methyl; R 2 , R 3 , R 5 , and R 7 are hydrogen; R 4 and R 8 are methyl, R 10 is an N-succinimidyloxycarbonyl group attached directly to the para-position of the phenoxy ring, and X is CH 3 SO 4 .
Claim 6
Dependent Claim
24 words
A method as recited in claim 1 wherein the effective amount of enhancer in the chemiluminescent reaction mixture is greater than 0.005% by weight.
Claim 7
Dependent Claim
24 words
A method as recited in claim 5 wherein the effective amount of enhancer in the chemiluminescent reaction mixture is greater than 0.01% by weight.
Claim 8
Dependent Claim
30 words
A method as recited in claim 1 wherein R 1 is C 14 -C 16 alkyl or alkenyl; R 2 , R 3 and R 4 are methyl or ethyl.
Claim 9
Dependent Claim
14 words
A method as recited in claim 7 wherein the cationic surfactant is hexadecyltrimethylammonium chloride.
Claim 10
Dependent Claim
14 words
A method as recited in claim 1 wherein the enhancer is a nonionic surfactant.
Claim 11
Dependent Claim
16 words
A method as recited in claim 9 wherein the nonionic surfactant is a polyoxyethylenated nonionic surfactant.
Claim 12
Dependent Claim
15 words
A method as recited in claim 1 wherein the sulfated primary alcohol is sodium dodecylsulfate.
Claim 13
Dependent Claim
72 words
In a chemiluscent immunoassasy utilizing an acridinium ester as a label wherein the improvement comprises oxidizing the acridinium ester in the presence of an effective amount of an enhancer selected from the group consisting of: (a) a quarternary amine cationic surfactant of formula: ##STR9## wherein R 1 is C 8 -C 20 alkyl or alkenyl; and R 2 , R 3 and R 4 are C 1 -C 4 alkyl or alkenyl.
Claim 14
Dependent Claim
72 words
In a chemiluscent immunoassasy utilizing an acridinium ester as a label wherein the improvement comprises oxidizing the acridinium ester in the presence of an effective amount of an enhancer selected from the group consisting of: (a) a quarternary amine cationic surfactant of formula: ##STR9## wherein R 1 is C 8 -C 20 alkyl or alkenyl; and R 2 , R 3 and R 4 are C 1 -C 4 alkyl or alkenyl.
Claim 15
Dependent Claim
32 words
A chemiluminescent assay as recited in claim 12 wherein R 1 is C 14 -C 16 alkyl or alkenyl; and R 2 , R 3 and R 4 are methyl or ethyl.
References Cited (Backward Citations)
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