US4931361A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cryoprotective reagents in freeze-drying membranes
Assignee: California Institute of Technology
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Filing: 1988-11-18
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Granted: 1990-06-05
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Expires: 2008-11-18
Abstract
Abstract The ability of phosphoglyceride-containing membranes to withstand the disruptive effects of freezing, thawing, or freeze-drying is enhanced when treated with (a) a compound of the formula R-X-R 1 , wherein R is a lipid or lipophilic anchor, X is a hydrophilic linker group, and R 1 is a polyalcohol or carbohydrate group, and (b) ascorbic acid.
Inventors
- 👤 John D. Baldeschwieler
- 👤 Raymond P. Goodrich, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 21:33
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; Y; Y10; Y10S; Y10S436/00; Y10S436/829; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
67 words
A composition for the protection of phosphoglyceride-containing biological or synthetic membranes from the disruptive effects of freezing, thawing, or freeze-drying, comprising (a) a compound of the formula R--X--R.sub.1 wherein R is a lipid or lipophilic anchor which is suitable for intercalation into and incorporation within a phosphoglyceride layer, X is a hydrophilic linker group, and R 1 is a polyalcohol or carbohydrate group, and (b) ascorbic acid.
Claim 2
Dependent Claim
67 words
A composition for the protection of phosphoglyceride-containing biological or synthetic membranes from the disruptive effects of freezing, thawing, or freeze-drying, comprising (a) a compound of the formula R--X--R.sub.1 wherein R is a lipid or lipophilic anchor which is suitable for intercalation into and incorporation within a phosphoglyceride layer, X is a hydrophilic linker group, and R 1 is a polyalcohol or carbohydrate group, and (b) ascorbic acid.
Claim 3
Dependent Claim
11 words
The composition of claim 1 wherein said anchor is a lipid.
Claim 4
Dependent Claim
11 words
The composition of claim 1 wherein said anchor is a steroid.
Claim 5
Dependent Claim
12 words
The composition of claim 1 wherein R 1 is a carbohydrate group.
Claim 6
Dependent Claim
11 words
The composition of claim 4 wherein R 1 is a disaccharide.
Claim 7
Dependent Claim
16 words
The composition of claim 1 wherein said ascorbic acid is in the form of sodium ascorbate.
Claim 8
Independent Claim
83 words
A method for the protection of phosphoglyceride-containing biological or synthetic membranes from the disruptive effects of freezing, thawing, or freeze-drying comprising applying to an aqueous solution of said membranes (a) from about 10 to about 40 mole% of a compound of the formula R--X--R.sub.1 wherein R is a lipid or lipophilic anchor which is suitable for intercalation into and incorporation within a phosphoglyceride layer, X is a hydrophilic linker group, and R 1 is a polyalcohol or carbohydrate group, and (b) ascorbic acid.
Claim 9
Independent Claim
83 words
A method for the protection of phosphoglyceride-containing biological or synthetic membranes from the disruptive effects of freezing, thawing, or freeze-drying comprising applying to an aqueous solution of said membranes (a) from about 10 to about 40 mole% of a compound of the formula R--X--R.sub.1 wherein R is a lipid or lipophilic anchor which is suitable for intercalation into and incorporation within a phosphoglyceride layer, X is a hydrophilic linker group, and R 1 is a polyalcohol or carbohydrate group, and (b) ascorbic acid.
Claim 10
Dependent Claim
24 words
The method of claim 7 wherein the concentration of said sodium ascorbate to said aqueous solution ranges from about 25 to about 500 mM.
Claim 11
Dependent Claim
24 words
The method of claim 8 wherein the concentration of said sodium ascorbate to said aqueous solution ranges from about 40 to about 100 mM.
Claim 12
Dependent Claim
11 words
The method of claim 7 wherein the anchor is a lipid.
Claim 13
Dependent Claim
11 words
The method of claim 7 wherein the anchor is a steroid.
Claim 14
Dependent Claim
12 words
The method of claim 7 wherein R 1 is a carbohydrate group.
Claim 15
Dependent Claim
11 words
the method of claim 12 wherein R 1 is a disaccharide.
Claim 16
Dependent Claim
16 words
The method of claim 7 wherein said ascorbic acid is in the form of sodium ascorbate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-06-04 | 1993-12-04 | 1994-06-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-06-04 | 1997-12-04 | 1998-06-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-06-04 | 2001-12-04 | 2002-06-05 | $7700.00 | Window Closed / Lapsed |