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US4932044A Expired / Lapsed US ⏱️ 0.0 years remaining

Tissue analyzer

Assignee: Yale University
•
Filing: 1988-11-04
•
Granted: 1990-06-05
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Expires: 2008-11-04
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Abstract

Abstract A system is disclosed for counting particles/cells within a counting box of precisely known volume that is completely inside a transparent section or sample. The box has a chosen height with defined upper and lower limits and appropriately selected width and depth dimensions. It resides completely within the sample and has no surface in common with an exterior surface of the sample. The system includes a compound light microscope that has a depth of focus which is small in relation to the thickness dimension of the counting box. The microscope includes adjustment means for moving the focal plane through a range which is greater than the height of the counting box. Display means are provided which show the portion of the sample that is within the depth of focus and user-operated means is provided to enable the user to mark the cells so displayed. Indicator means are further provided to either audibly or visually indicate to the user when the adjustment means cause the focal plane to pass beyond either the upper or lower height limits of the counting box. The indicator means further includes means for accumulating a count of cells within the counting volume as the user operates the marker means. Means are also provided to compensate for optical foreshortening.

Inventors

  • 👤 Robert W. Williams
  • 👤 Pasko Rakic

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-31 03:09
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1468; G06; G06T; G06T7/00; G06T7/0002; G06T7/0012; G01N15/1429; G01N15/1433; G06T2207/00; G06T2207/30; G06T2207/30004; G06T2207/30024

Claims Analysis

Total Claims: 8
Claim 1
Independent Claim 138 words
In a system for counting particles within an interior volume of a transparent sample, said volume having chosen thickness and width dimensions, said thickness dimension exhibiting upper and lower height limits, said interior volume containing no surface coincident with an exterior surface of said sample, the combination comprising: a compound light microscope with a focal plane whose depth of field is small in relation to said thickness dimension, said microscope having adjustment means for moving said sample through a range greater than said thickness dimension; display means for showing particles in said sample which are within said focal plane; marker means for a marking a particle to be counted; indicator means for indicating when said adjustment means causes said focal plane to overlap an upper or lower height limit; and means for accumulating a count of marked particles.
Claim 2
Dependent Claim 138 words
In a system for counting particles within an interior volume of a transparent sample, said volume having chosen thickness and width dimensions, said thickness dimension exhibiting upper and lower height limits, said interior volume containing no surface coincident with an exterior surface of said sample, the combination comprising: a compound light microscope with a focal plane whose depth of field is small in relation to said thickness dimension, said microscope having adjustment means for moving said sample through a range greater than said thickness dimension; display means for showing particles in said sample which are within said focal plane; marker means for a marking a particle to be counted; indicator means for indicating when said adjustment means causes said focal plane to overlap an upper or lower height limit; and means for accumulating a count of marked particles.
Claim 3
Dependent Claim 29 words
The system as recite din claim 1 wherein said compound light microscope is provided with differential interference contrast optics to enhance the particles which are within said focal plane.
Claim 4
Dependent Claim 35 words
The system of claim 1 further comprising: means for altering the showing of said display means when said adjustment means is operated to cause said focal plane to overlap an upper or lower height limit.
Claim 5
Dependent Claim 43 words
The system of claim 3 wherein said altering means modifies said display means in a first fashion when said overlap of said upper height limit occurs and modifies said display means in a second fashion when said overlap of said lower limit occurs.
Claim 6
Dependent Claim 25 words
The system of claim 4 wherein said first fashion causes said display means to exhibit a first color and said second fashion a second color.
Claim 7
Dependent Claim 37 words
The system of claim 1 wherein said indicator means comprises alarm means which provides audible tones when said adjustment means is operated so as to cause said focal plane to overlap an upper or lower height limit.
Claim 8
Dependent Claim 35 words
The system of claim 6 wherein said alarm means provides a first audible tone when said overlap of said upper limit occurs and a different audible tone when said overlap of said lower limit occurs.

References Cited (Backward Citations)

US3721759A Cited
US4176376A Cited
US4667335A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1993-06-04 1993-12-04 1994-06-05 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1997-06-04 1997-12-04 1998-06-05 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2001-06-04 2001-12-04 2002-06-05 $7700.00 Window Closed / Lapsed

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