US4945060A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Device for detecting microorganisms
Assignee: Biomerieux Inc
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Filing: 1988-03-15
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Granted: 1990-07-31
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Expires: 2008-03-15
Abstract
Abstract An instrument and a sealable, sterilizable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium and a sensor means with an indicator medium therein. Changes in the indicator medium resulting from pH change or change in CO 2 concentration in the medium are detected from outside the vessel.
Inventors
- 👤 James E. Turner
- 👤 Thurman C. Thorpe
- 👤 James L. Di Guiseppi
- 👤 Richard C. Driscoll
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 17:34
CPC Classifications
C; C12; C12Q; C12Q1/00; C12M; C12M41/00; C12M41/26; C12M1/00; C12M1/34; C12Q1/02; C12Q1/04
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
136 words
A device for continuously monitoring biological activity in a specimen comprising a sealable, sterilizable, specimen container, having an internal chamber in which a specimen may be cultured with a sterile culture medium, having at least one transparent section in said container and a sensor means located inside said container in the region of the transparent section, whereby changes in the appearance of the sensor means can be continuously monitored from the exterior of said container through said transparent section, thereby monitoring biological activity without violating the integrity of said container after sealing, said sensor means comprising a membrane and a non-fluorescent indicator medium, the indicator medium being selected for its ability to exhibit a detectable change when exposed to products of an organism's metabolic activity, wherein the sensor is sterilizable by heating without affecting its function.
Claim 2
Independent Claim
136 words
A device for continuously monitoring biological activity in a specimen comprising a sealable, sterilizable, specimen container, having an internal chamber in which a specimen may be cultured with a sterile culture medium, having at least one transparent section in said container and a sensor means located inside said container in the region of the transparent section, whereby changes in the appearance of the sensor means can be continuously monitored from the exterior of said container through said transparent section, thereby monitoring biological activity without violating the integrity of said container after sealing, said sensor means comprising a membrane and a non-fluorescent indicator medium, the indicator medium being selected for its ability to exhibit a detectable change when exposed to products of an organism's metabolic activity, wherein the sensor is sterilizable by heating without affecting its function.
Claim 3
Dependent Claim
25 words
The device of claim 1, wherein the indicator medium comprises at least one compound selected from the group consisting of bromothymol blue, and xylenol blue.
Claim 4
Dependent Claim
21 words
The device of claim 1, wherein the membrane of the sensor means is bonded to the interior surface of said container.
Claim 5
Dependent Claim
20 words
The device of claim 3, wherein the membrane is bonded to the interior surface of said container using an adhesive.
Claim 6
Dependent Claim
41 words
The device of claim 3, wherein the membrane of the sensor means is at least circumferentially bonded to the interior surface of the container, whereby neither the specimem nor the culture medium can pase between the membrane and the container surface.
Claim 7
Dependent Claim
11 words
The device of claim 1, which has been sterilized by autoclaving.
Claim 8
Dependent Claim
21 words
The device of claim 1, wherein the sensor means is separated from the specimen in said container by a semipermeable membrane.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-07-30 | 1994-01-29 | 1994-07-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-07-30 | 1998-01-29 | 1998-07-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-07-30 | 2002-01-29 | 2002-07-31 | $7700.00 | Window Closed / Lapsed |