US4956455A
Expired / Lapsed
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Bovine fibroblast growth factor
Assignee: Salk Institute for Biological Studies
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Filing: 1985-06-20
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Granted: 1990-09-11
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Expires: 2005-06-20
Abstract
Abstract Substantially pure bovine pituitary fibroblast growth factor, a 146 amino acid residue polypeptide, is produced. The amino acid residue sequence of bpFGF is disclosed as well as a DNA chain encoding the polypeptide. By appropriately inserting a synthesized DNA chain into a cloning vector and using the cloning vector to transform cells, synthetic bpFGF can be obtained from transformed cell lines, both prokaryotic and eukaryotic.
Inventors
- 👤 Andrew Baird
- 👤 Frederick S. Esch
- 👤 Peter Bohlen
- 👤 Denis Gospodarowicz
- 👤 Nicholas C. Ling
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 14:13
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/50; C07K14/503; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/827; Y10S530/839
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
12 words
Substantially pure basic fibroblast growth factor containing the amino acid sequence: ##STR3##
Claim 2
Dependent Claim
12 words
Substantially pure basic fibroblast growth factor containing the amino acid sequence: ##STR3##
Claim 3
Dependent Claim
33 words
Basic fibroblast growth factor which is substantially pure, which is capable of stimulating proliferation of endothelial cells, and which has a MW of about 16,250, being a polypeptide containing about 140 amino acids.
Claim 4
Dependent Claim
33 words
Basic fibroblast growth factor which is substantially pure, which is capable of stimulating proliferation of endothelial cells, and which has a MW of about 16,250, being a polypeptide containing about 140 amino acids.
Claim 5
Dependent Claim
15 words
Basic fibroblast growth factor according to claim 2 wherein the N-terminus has the sequence Pro-Ala-Leu-Pro-Glu-Asp-Gly-Gly-Ser.
Claim 6
Dependent Claim
12 words
Basic fibroblast growth factor according to claim 3 isolated from bovine pituitaries.
Claim 7
Dependent Claim
13 words
Basic fibroblast growth factor according to claim 2 isolated from bovine brain tissue.
Claim 8
Dependent Claim
36 words
Basic fibroblast growth factor according to claim 3 isolated from bovine pituitaries wherein the polypeptide has about the following amino acid composition: Asx(12), Thr(4), Ser(9), Glx(14), Pro(9) Gly(16),Ala(9), Cys(4), Val(6), Met(2), Ile(3), Leu(13),Tyr(7),Phe(7),His(2), Trp(1),Lys(14), and Arg(12).
Claim 9
Dependent Claim
33 words
Basic fibroblast growth factor according to claim 2, which is capable of stimulating proliferation of endothelial cells in in vitro culture to at least about 5 times normal level in about 4 days.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-09-10 | 1994-03-12 | 1994-09-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-09-10 | 1998-03-12 | 1998-09-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-09-10 | 2002-03-12 | 2002-09-11 | $7700.00 | Window Closed / Lapsed |