US4960715A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic test methods
Assignee: Remel Inc
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Filing: 1988-02-25
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Granted: 1990-10-02
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Expires: 2008-02-25
Abstract
Abstract Agglutination assays, particularly latex agglutination assays, for simultaneous testing for a multiplicity of ligands. The reagents for use in the assays comprise two or more insoluble colored substances, each substance being adapted to form a distinctively colored agglutinate in the presence of a specific ligand or specific group of ligands.
Inventors
- 👤 Susan G. Hadfield
- 👤 Franklin E. A. Norrington
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 02:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/53; G01N33/531; G01N33/532; G01N33/58; G01N33/585; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
183 words
A method of testing for the presence of a ligand in an aqueous liquid medium comprising the steps of: A. mixing a sample of the said medium with a reagent comprising particles of a first colour having attached thereto a first monoclonal antibody bindable to the said ligand, in admixture with particles of a second colour having attached thereto a second monoclonal antibody obtained from a cell line derived from a non-inoculated animal of the same species as that from which was derived the cell line from which the said first monoclonal antibody was obtained, whereby non-specific interaction between the said sample and the said reagent is indicated by agglutination of both the said particles of the first colour and the said particles of the second colour and the presence of the said ligand in the said sample, free from said non-specific interaction, is indicated by agglutination of the said particles of the first colour alone; B. after an appropriate period of time, inspecting the mixture resulting from step A.; and C. determining the test result from the appearance of the said mixture.
Claim 2
Independent Claim
183 words
A method of testing for the presence of a ligand in an aqueous liquid medium comprising the steps of: A. mixing a sample of the said medium with a reagent comprising particles of a first colour having attached thereto a first monoclonal antibody bindable to the said ligand, in admixture with particles of a second colour having attached thereto a second monoclonal antibody obtained from a cell line derived from a non-inoculated animal of the same species as that from which was derived the cell line from which the said first monoclonal antibody was obtained, whereby non-specific interaction between the said sample and the said reagent is indicated by agglutination of both the said particles of the first colour and the said particles of the second colour and the presence of the said ligand in the said sample, free from said non-specific interaction, is indicated by agglutination of the said particles of the first colour alone; B. after an appropriate period of time, inspecting the mixture resulting from step A.; and C. determining the test result from the appearance of the said mixture.
Claim 3
Dependent Claim
24 words
The method of claim 1 wherein both the particles of the first colour and the particles of the second colour are of polystyrene latex.
Claim 4
Dependent Claim
12 words
The method of claim 1 wherein the ligand is a viral antigen.
Claim 5
Dependent Claim
12 words
The method of claim 1 wherein the ligand is a bacterial antigen.
Claim 6
Dependent Claim
15 words
The method of claim 1 wherein the ligand is an antigen characteristic of Haemophilus organisms.
Claim 7
Dependent Claim
15 words
The method of claim 1 wherein the ligand is an antigen characteristic of Neisseria organisms.
Claim 8
Dependent Claim
15 words
The method of claim 1 wherein the ligand is an antigen characteristic of Salmonella organisms.
Claim 9
Dependent Claim
15 words
The method of claim 1 wherein the ligand is an antigen characteristic of Streptococcus organisms.
Claim 10
Dependent Claim
18 words
The method of claim 1 wherein the ligand is an antigen characteristic of Streptococcus organisms of serogroup A.
Claim 11
Dependent Claim
145 words
A test reagent for the presence of a ligand in a sample of an aqueous liquid medium, said reagent comprising particles of a first colour having attached thereto a first monoclonal antibody bindable to the said ligand, in admixture with particles of a second colour having attached thereto a second monoclonal antibody obtained from a cell line derived from a non-inoculated animal of the same species as that from which was derived the cell line from which the said first monoclonal antibody was obtained, whereby non-specific interaction between the said sample and the said reagent is indicated by agglutination of both the said particles of the first colour and the said particles of the second colour and the presence of the said ligand in the said sample, free from said non-specific interaction, is indicated by agglutination of the said particles of the first colour alone.
Claim 12
Dependent Claim
145 words
A test reagent for the presence of a ligand in a sample of an aqueous liquid medium, said reagent comprising particles of a first colour having attached thereto a first monoclonal antibody bindable to the said ligand, in admixture with particles of a second colour having attached thereto a second monoclonal antibody obtained from a cell line derived from a non-inoculated animal of the same species as that from which was derived the cell line from which the said first monoclonal antibody was obtained, whereby non-specific interaction between the said sample and the said reagent is indicated by agglutination of both the said particles of the first colour and the said particles of the second colour and the presence of the said ligand in the said sample, free from said non-specific interaction, is indicated by agglutination of the said particles of the first colour alone.
Claim 13
Dependent Claim
24 words
The reagent of claim 10 wherein both the particles of the first colour and the particles of the second colour are of polystyrene latex.
Claim 14
Dependent Claim
12 words
The reagent of claim 10 wherein the ligand is a viral antigen.
Claim 15
Dependent Claim
12 words
The reagent of claim 10 wherein the ligand is a bacterial antigen.
Claim 16
Dependent Claim
15 words
The reagent of claim 10 wherein the ligand is an antigen characteristic of Haemophilus organisms.
Claim 17
Dependent Claim
15 words
The reagent of claim 10 wherein the ligand is an antigen characteristic of Neisseria organisms.
Claim 18
Dependent Claim
15 words
The reagent of claim 10 wherein the ligand is an antigen characteristic of Salmonella organisms.
Claim 19
Dependent Claim
15 words
The reagent of claim 10 wherein the ligand is an antigen characteristic of Streptococcus organisms.
Claim 20
Dependent Claim
18 words
The reagent of claim 10 wherein the ligand is an antigen characteristic of Streptococcus organisms of serogroup A.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-01 | 1994-04-02 | 1994-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-01 | 1998-04-02 | 1998-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-01 | 2002-04-02 | 2002-10-02 | $7700.00 | Window Closed / Lapsed |