US4965185A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for low-temperature preservation of embryos
Assignee: INSTITUT PROBLEM KRIOBIOLOGII I KRIOMEDITSINY AN SSSR
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Filing: 1988-06-21
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Granted: 1990-10-23
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Expires: 2008-06-21
Abstract
Abstract The method consists in cooling of embryos down to the temperature of the refrigerant in a buffer-salt fluid, the cooling process being carried out in a temperature diffusive powdered material that has preliminarily been cooled down to the refrigerant temperature. The buffer-salt fluid comprises a mixture of cryoprotectors, i.e., glycerol, dimethylsulphoxide, and a dihydric alcohol. An example of the temperature diffusive material is powdered metal.
Inventors
- 👤 Valentin I. Grischenko
- 👤 Jury V. Kalugin
- 👤 Nina A. Luchko
- 👤 Elena N. Chernysh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 06:22
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
93 words
A method for cooling embryos to the temperature of a refrigerant in which they are to be stored comprising: adding said embryos to a buffer-salt medium; said buffer-salt medium comprising a mixture of cryoprotectors, said cryoprotectors selected from the group consisting of glycerol, dimethylsulphoxide and a dihdric alcohol; placing said embryos in said buffer-salt medium into a container made of food packing foil; introducing said container with said embryos into a thermal diffusive powdered material; said powdered material having been cooled to the temperature of the refrigerant; said powdered material being a metal
Claim 2
Independent Claim
93 words
A method for cooling embryos to the temperature of a refrigerant in which they are to be stored comprising: adding said embryos to a buffer-salt medium; said buffer-salt medium comprising a mixture of cryoprotectors, said cryoprotectors selected from the group consisting of glycerol, dimethylsulphoxide and a dihdric alcohol; placing said embryos in said buffer-salt medium into a container made of food packing foil; introducing said container with said embryos into a thermal diffusive powdered material; said powdered material having been cooled to the temperature of the refrigerant; said powdered material being a metal
Claim 3
Dependent Claim
17 words
A method as claimed in claim 1, wherein zinc is used as the thermal diffusive powdered material.
Claim 4
Dependent Claim
13 words
A method as claimed in claim 1, wherein the dihydric alcohol is 1,2-propanediol.
Claim 5
Dependent Claim
32 words
A method as claimed in claim 1, wherein liquid nitrogen is used as the refrigerant, and each of the three cryoprotectors is used in a concentration of from 4.75 to 5.52 percent.
Claim 6
Dependent Claim
19 words
A method as claimed in claim 1, wherein each of the three cryoprotectors is used in a 1:1 ratio.
Claim 7
Dependent Claim
14 words
A method as claimed in claim 1, wherein the buffer-salt fluid contains choline chlorine.
Claim 8
Dependent Claim
17 words
A method as claimed in claim 6 wherein the choline-chloride solution has a concentration of 0.4 percent.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-22 | 1994-04-23 | 1994-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-22 | 1998-04-23 | 1998-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-22 | 2002-04-23 | 2002-10-23 | $7700.00 | Window Closed / Lapsed |