US4973549A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Quantitative diagnostic assay employing signal producing agent bound to support and measuring migration distance of detectable signal
Assignee: CHEMTRAK
•
Filing: 1987-06-22
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Granted: 1990-11-27
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Expires: 2007-06-22
Abstract
Abstract Methods and kits are provided for detecting analytes, which can be measured directly or indirectly, by production of an enzymatic product. The enzymatic product reacts with a signal producing reagent bound to a bibulous strip. Various devices are employed for producing a reaction, directly or indirectly, with the analyte and then providing for migration of the product through the bibulous strip with reaction with the reagent to produce a detectable signal. The distance from a pre-determined site to the border of the detectable signal is a quantitative measure of the analyte in the sample.
Inventors
- 👤 Pyare Khanna
- 👤 Prithipal Singh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 19:31
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/904
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
195 words
A method for determining total cholesterol present in a blood or serum sample as cholesterol or cholesterol esters, employing a reagent system which reacts with said cholesterol and cholesterol esters to produce hydrogen peroxide which reacts with a chromogen reagent produce a detectable signal, and employing a bibulous support comprising a calibrated measurement zone to which said chromogen reagent is bound, said method comprising: contacting at an assay mixture receiving site on said support an assay mixture comprising a sample suspected of containing said cholesterol and cholesterol esters in a liquid medium and an enzyme reaction system comprising cholesterol esterase and cholesterol oxidase whereby said sample and said enzyme reaction system react substantially to completion to produce said hydrogen peroxide; dipping one end of said bibulous support distant from said measurement zone into a solution comprising horseradish peroxidase, whereby said solution migrates through said assay mixture receiving site into said measurement zone whereby said hydrogen peroxide reacts with said chromogen to produce a detectable signal; and measuring in said calibrated measurement zone the furthest distance from said receiving site of the detectable signal as a measure of the amount of total cholesterol in said sample.
Claim 2
Independent Claim
195 words
A method for determining total cholesterol present in a blood or serum sample as cholesterol or cholesterol esters, employing a reagent system which reacts with said cholesterol and cholesterol esters to produce hydrogen peroxide which reacts with a chromogen reagent produce a detectable signal, and employing a bibulous support comprising a calibrated measurement zone to which said chromogen reagent is bound, said method comprising: contacting at an assay mixture receiving site on said support an assay mixture comprising a sample suspected of containing said cholesterol and cholesterol esters in a liquid medium and an enzyme reaction system comprising cholesterol esterase and cholesterol oxidase whereby said sample and said enzyme reaction system react substantially to completion to produce said hydrogen peroxide; dipping one end of said bibulous support distant from said measurement zone into a solution comprising horseradish peroxidase, whereby said solution migrates through said assay mixture receiving site into said measurement zone whereby said hydrogen peroxide reacts with said chromogen to produce a detectable signal; and measuring in said calibrated measurement zone the furthest distance from said receiving site of the detectable signal as a measure of the amount of total cholesterol in said sample.
Claim 3
Dependent Claim
11 words
A method according to claim 1 wherein said chromogen is 4-chloro-1-naphthol.
Claim 4
Dependent Claim
22 words
A method according to claim 2 wherein said assay mixture receiving site is proximal to said one end of said bibulous support.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-11-26 | 1994-05-28 | 1994-11-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-11-26 | 1998-05-28 | 1998-11-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-11-26 | 2002-05-28 | 2002-11-27 | $7700.00 | Window Closed / Lapsed |