US5019504A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of taxol or taxol-like compounds in cell culture
Assignee: United States Department of Agriculture
•
Filing: 1989-03-23
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Granted: 1991-05-28
•
Expires: 2009-03-23
Abstract
Abstract Tissue of Taxus brevifolia has been successfully cultured to produce taxol, related alkaloids, and alkaloid precursors. These procedures will provide a supply of chemotherapeutic agents.
Inventors
- 👤 Alice A. Christen
- 👤 Donna M. Gibson
- 👤 John Bland
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 17:59
CPC Classifications
A; A01; A01H; A01H4/00; C; C12; C12N; C12N5/00; C12N5/0018; C12N5/0025; C12P; C12P17/00; C12P17/02
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
70 words
A method of producing taxane ring-containing alkaloid compounds from cell cultures of Taxus brevifolia comprising the steps of: a. providing living tissue of said Taxus brevifolia; b. providing a nutrient culture medium suitable for said cell culture formation from said tissue; c. culturing said tissue on said medium to produce calli or cell suspensions derived therefrom and d. recovering said taxane ring-containing alkaloid compounds from said calli or suspension culture.
Claim 2
Independent Claim
70 words
A method of producing taxane ring-containing alkaloid compounds from cell cultures of Taxus brevifolia comprising the steps of: a. providing living tissue of said Taxus brevifolia; b. providing a nutrient culture medium suitable for said cell culture formation from said tissue; c. culturing said tissue on said medium to produce calli or cell suspensions derived therefrom and d. recovering said taxane ring-containing alkaloid compounds from said calli or suspension culture.
Claim 3
Dependent Claim
27 words
The method as described in claim 1 wherein said living tissue in step (a) is selected from the group consisting of bark, cambial tissue, needles, and roots.
Claim 4
Dependent Claim
14 words
The method as described in claim 1 wherein said recovery is from said calli.
Claim 5
Dependent Claim
15 words
The method as described in claim 1 wherein said recovery is from said cell suspension.
Claim 6
Dependent Claim
22 words
The method as described in claim 1 wherein said cell culture comprises cells held in a matrix formed by a gelling agent.
Claim 7
Dependent Claim
14 words
The method as described in claim 5 wherein said matrix is formed from agar.
Claim 8
Dependent Claim
15 words
The method as described in claim 1 wherein said cell culture is encapsulated into capsules.
Claim 9
Dependent Claim
15 words
The method as described in claim 7 wherein said capsules are formed from calcium alginate.
Claim 10
Dependent Claim
13 words
The method as described in claim 1 wherein said alkaloid compound is taxol.
Claim 11
Dependent Claim
23 words
The method as described in claim 1 wherein an inducer is added to step (c) in an amount sufficient to increase alkaloid production.
Claim 12
Dependent Claim
23 words
The method as described in claim 10 wherein said inducer is autoclaved mycelia or sterilized culture filtrates of Cytospora abietis, ATCC No. 38688.
Claim 13
Dependent Claim
23 words
The method as described in claim 10 wherein said inducer is autoclaved mycelia or sterilized culture filtrates of Penicillium minioluteum (Dierckx), NRRL 18467.
Claim 14
Dependent Claim
13 words
The method as described in claim 10 wherein said inducer is vanadyl sulfate.
Claim 15
Dependent Claim
26 words
The method as described in claim 1 wherein said medium is Gamborg's B5, with 2,4-dichlorophenoxy acetic acid or naphthalene acetic acid added as a growth hormone.
Claim 16
Dependent Claim
15 words
The method as described in claim 1 wherein said medium is Lloyd and McCowns medium.
Claim 17
Dependent Claim
25 words
The method as described in claim 1 wherein said culture medium contains an antimicrobial agent selected from the group consisting of cefoxitin, benlate, and cloxacillin.
Claim 18
Dependent Claim
36 words
The method as described in claim 1 wherein said recovery is accomplished by adsorbing said alkaloid compounds onto polymeric adsorbent beads, separating said beads from said culture medium, and extracting said alkaloid compounds from said beads.
Claim 19
Dependent Claim
14 words
The method as described in claim 1 wherein said culture medium contains ammonium nitrate.
References Cited (Backward Citations)
US4152214A
Cited
US4910138A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-05-27 | 1994-11-26 | 1995-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-27 | 1998-11-26 | 1999-05-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-05-27 | 2002-11-26 | 2003-05-28 | $7700.00 | Window Closed / Lapsed |