US5019648A
Expired / Lapsed
US
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Monoclonal antibody specific for the adhesion function domain of a phagocyte cell surface protein
Assignee: Dana Farber Cancer Institute Inc
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Filing: 1988-03-07
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Granted: 1991-05-28
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Expires: 2008-03-07
Abstract
Abstract A hybrid cell line is developed which produces a monoclonal antibody which binds to a unique antigenic site expressed on the surface of phagocytic cells. The monoclonal antibody binds to and activates a specific domain of the CD11b glycoprotein so as to inhibit adhesion dependent functions of the phagocytic cell, but it does not affect other phagocytic functions. This monoclonal antibody can be used as a reactant in an in vitro diagnostic immunoassay for detecting the unique antigenic site on the surface of normal human neutrophils.
Inventors
- 👤 Stuart F. Schlossman
- 👤 James D. Griffin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 10:13
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/78; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/2839; C07K16/2845; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/828
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
60 words
A monoclonal antibody which binds specifically to the epitope defined by the MY904 antibody, wherein said binding inhibits only the adhesion-dependent functions of granulocytes and monocytes without binding the iC3b component of complement, and wherein the antibody does not bind human T and B lymphocytes, said MY904 antibody being the antibody produced by the hybridoma having ATCC accession number HB9510.
Claim 2
Dependent Claim
60 words
A monoclonal antibody which binds specifically to the epitope defined by the MY904 antibody, wherein said binding inhibits only the adhesion-dependent functions of granulocytes and monocytes without binding the iC3b component of complement, and wherein the antibody does not bind human T and B lymphocytes, said MY904 antibody being the antibody produced by the hybridoma having ATCC accession number HB9510.
Claim 3
Dependent Claim
11 words
A hybridoma cell line which produces the antibody of claim 1.
Claim 4
Dependent Claim
23 words
The cell line of claim 2 wherein said monoclonal antibody binds specifically to granulocytes from peripheral blood samples of chronic granulocytic leukemia origin.
Claim 5
Dependent Claim
32 words
The cell line of claim 2 wherein the monoclonal antibody binds specifically to normal monocytes, normal granulocytes and normal bone marrow mononuclear cells and does not bind to normal erthrocytes and platelets.
Claim 6
Dependent Claim
42 words
The cell line produced by a hydridoma technique having all the identifying characteristics of the sample on deposit with the American Type Culture Collection No. HB9510 producing monoclonal antibody MY904 that specifically binds to the adhesion-domain of the CD116/CD18 phagocyte surface protein.
Claim 7
Dependent Claim
42 words
The cell line produced by a hydridoma technique having all the identifying characteristics of the sample on deposit with the American Type Culture Collection No. HB9510 producing monoclonal antibody MY904 that specifically binds to the adhesion-domain of the CD116/CD18 phagocyte surface protein.
Claim 8
Dependent Claim
29 words
The monoclonal antibody of claim 1 which binds specifically to normal monocytes, normal granulocytes and normal bone marrow mononuclear cells and does not bind to normal erythrocytes and platelets.
Claim 9
Dependent Claim
16 words
The monoclonal antibody of claim 1 which does not bind specifically to normal erythrocytes and platelets.
Claim 10
Dependent Claim
24 words
The monoclonal antibody of claim 1 produced by the hybrid cell line having all the identifying characteristics of the A.T.C.C. deposit No. HB 9510.
Claim 11
Dependent Claim
17 words
The monoclonal antibody of claim 1 which does not bind specifically to human T or B lymphocytes.
References Cited (Backward Citations)
US4840793A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-05-27 | 1994-11-26 | 1995-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-27 | 1998-11-26 | 1999-05-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-05-27 | 2002-11-26 | 2003-05-28 | $7700.00 | Window Closed / Lapsed |