US5047329A
Expired / Lapsed
US
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Method for the measurement of creatine or creatinine and reagents for these measurements
Assignee: Noda Institute for Scientific Research
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Filing: 1988-01-05
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Granted: 1991-09-10
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Expires: 2008-01-05
Abstract
Abstract A method for measuring creatine in a sample by the use of creatine amidinohydrolase which comprises decomposing the N-ethylglycine present in the sample enzymatically and thereafter reacting sarcosine oxidase upon the sample; and a reagent for use in the measurement of creatine comprising the first reagent and the second reagent, wherein the first reagent comprises a sarcosine oxidase of which Km value to N-ethylglycine at pH 8, 37° C. is 20 mM or below and catalase or comprises said sarcosine oxidase, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatine amidinohydrolase, a sarcosin oxidase of which Km value to N-ethylglycine at pH 8, 37° C. is 50 mM or above, peroxidase and a color reagent for H 2 O 2 .
Inventors
- 👤 Masaru Suzuki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 07:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/902; G01N2333/906; G01N2333/914; G01N2333/978; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10T; Y10T436/00; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
94 words
A method for measuring creatine in a sample comprising: decomposing N-ethylglycine present in the sample enzymatically with sarcosine oxidase of which Km value (Michaelis constant) to N-ethylglycine at pH 8, 37° C. is 20 mM or below and thereafter determining creatine by reacting the decomposed sample with creatine amidinohydrolase and sarcosine oxidase N of which Km value (Michaelis constant) to N-ethylglycine at pH 8, 37° C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671) and measuring final reaction products as a measure of creatine present in the sample.
Claim 2
Independent Claim
94 words
A method for measuring creatine in a sample comprising: decomposing N-ethylglycine present in the sample enzymatically with sarcosine oxidase of which Km value (Michaelis constant) to N-ethylglycine at pH 8, 37° C. is 20 mM or below and thereafter determining creatine by reacting the decomposed sample with creatine amidinohydrolase and sarcosine oxidase N of which Km value (Michaelis constant) to N-ethylglycine at pH 8, 37° C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671) and measuring final reaction products as a measure of creatine present in the sample.
Claim 3
Independent Claim
99 words
A method for measuring creatinine in a sample comprising decomposing creatine and N-ethylglycine present in the sample enzymatically with creatine amidinohydrolase and sarcosine oxidase of which Km value (Michaelis constant) to N-ethylglycine at pH 8, 37° C. is 20 mM or below, and thereafter determining creathinine by reacting the decomposed sample with creatinine amidinohydrolase and sarcosine oxidase N of which Km value (Michaelis constant) to N-ethylglycine at pH 8, 37° C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671) and measuring final reaction products as a measure of creatinine present in the sample.
Claim 4
Independent Claim
99 words
A method for measuring creatinine in a sample comprising decomposing creatine and N-ethylglycine present in the sample enzymatically with creatine amidinohydrolase and sarcosine oxidase of which Km value (Michaelis constant) to N-ethylglycine at pH 8, 37° C. is 20 mM or below, and thereafter determining creathinine by reacting the decomposed sample with creatinine amidinohydrolase and sarcosine oxidase N of which Km value (Michaelis constant) to N-ethylglycine at pH 8, 37° C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671) and measuring final reaction products as a measure of creatinine present in the sample.
Claim 5
Dependent Claim
106 words
A reagent for use in the measurement of creatine comprising the first reagent and the second reagent, wherein the first reagent comprises a sarcosine oxidase of which Km value to N-ethylglycine at pH 8, 37° C. is 20 mM or below and catalase or comprises the same sarcosine oxidase as above, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatine amidinohydrolase, sarcosine oxidase N of which Km value to N-ethylglycine at pH 8, 37° C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671) peroxidase and a color reagent for H 2 O 2 .
Claim 6
Dependent Claim
106 words
A reagent for use in the measurement of creatine comprising the first reagent and the second reagent, wherein the first reagent comprises a sarcosine oxidase of which Km value to N-ethylglycine at pH 8, 37° C. is 20 mM or below and catalase or comprises the same sarcosine oxidase as above, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatine amidinohydrolase, sarcosine oxidase N of which Km value to N-ethylglycine at pH 8, 37° C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671) peroxidase and a color reagent for H 2 O 2 .
Claim 7
Dependent Claim
111 words
A reagent for use in the measurement of creatinine comprising the first reagent and the second reagent, wherein the first reagent comprises creatine amidinohydrolasee, a sarconsine oxidase of which Km value to N-ethylglycine at pH 8, 37° C. is 20 mM or below and catalase or comprises the same creatine amidinohydrolase and sarcosine oxidase as above, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatinine amidohydrolase, sarcosine oxidase N of which Km value to N-ethylglycine at pH 8, 37° C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671); peroxidase and a color reagent for H 2 O 2 .
Claim 8
Dependent Claim
111 words
A reagent for use in the measurement of creatinine comprising the first reagent and the second reagent, wherein the first reagent comprises creatine amidinohydrolasee, a sarconsine oxidase of which Km value to N-ethylglycine at pH 8, 37° C. is 20 mM or below and catalase or comprises the same creatine amidinohydrolase and sarcosine oxidase as above, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatinine amidohydrolase, sarcosine oxidase N of which Km value to N-ethylglycine at pH 8, 37° C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671); peroxidase and a color reagent for H 2 O 2 .
Claim 9
Dependent Claim
20 words
A method according to claim 1 wherein the measuring of creatine is carried out in liquid state or dry state.
Claim 10
Dependent Claim
19 words
A method according to claim 2 wherein the measuring of creatinine is carried out in liquid or dry state.
Claim 11
Dependent Claim
16 words
A method according to claim 5 wherein the measuring is carried out in the dry state.
Claim 12
Dependent Claim
16 words
A method according to claim 6 wherein the measuring is carried out in the dry state.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-09-09 | 1995-03-11 | 1995-09-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-09-09 | 1999-03-11 | 1999-09-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-09-09 | 2003-03-11 | 2003-09-10 | $7700.00 | Window Closed / Lapsed |