US5059525A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Dry reagent for blood coagulation tests
Assignee: Roche Diagnostics GmbH
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Filing: 1990-05-29
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Granted: 1991-10-22
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Expires: 2010-05-29
Abstract
Abstract The present invention provides a dry reagent for blood coagulation tests in which an at least partial course of the coagulation cascade takes place, comprising a carrier material which contains a chromophoric substrate of a protease of the blood coagulation system, at least one factor and/or co-factor of the blood coagulation system and a buffer.
Inventors
- 👤 Knut Bartl
- 👤 Udo Becker
- 👤 Helmut Lill
- 👤 Hans Wielinger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 02:40
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/56; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/807; Y10S436/81; Y10S530/00; Y10S530/802
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
150 words
Test strip useful in carrying out a Quick Test, a partial prothromboplastin time test, or in an analysis of one of prothrombin, Factor VII, Factor VIII, Factor IX and Factor X, comprising: (i) a carrier material containing (ii) a protease which reacts with prothrombin, Factor VII, Factor VIII, Factor IX or Factor X to yield an activated protease; (iii) a chromophoric protease substrate cleavable by said activated protease in a reaction which does not change the course of the intrinsic or extrinsic coagulation cascade system of blood in the presence of said carrier material, (iv) a buffer, and (v) at least one additional member of the intrinsic or extrinsic coagulation cascade system, wherein said protease reacts with prothrombin, Factor VII, Factor VIII, Factor IX or Factor X in a reaction which is part of the intrinsic or extrinsic coagulation cascade system wherein (ii), (v) are dried on said carrier material.
Claim 2
Dependent Claim
150 words
Test strip useful in carrying out a Quick Test, a partial prothromboplastin time test, or in an analysis of one of prothrombin, Factor VII, Factor VIII, Factor IX and Factor X, comprising: (i) a carrier material containing (ii) a protease which reacts with prothrombin, Factor VII, Factor VIII, Factor IX or Factor X to yield an activated protease; (iii) a chromophoric protease substrate cleavable by said activated protease in a reaction which does not change the course of the intrinsic or extrinsic coagulation cascade system of blood in the presence of said carrier material, (iv) a buffer, and (v) at least one additional member of the intrinsic or extrinsic coagulation cascade system, wherein said protease reacts with prothrombin, Factor VII, Factor VIII, Factor IX or Factor X in a reaction which is part of the intrinsic or extrinsic coagulation cascade system wherein (ii), (v) are dried on said carrier material.
Claim 3
Dependent Claim
60 words
The test strip of claim 1 further comprising a second carrier material comprising an oxidizing agent, and said first carrier material further comprises an aniline or phenol derivative which forms a colored compound with the chromophoric protease substrate after said chromophoric protease substrate has been cleaved by said protease to release a chromophore in the presence of said oxidizing agent.
Claim 4
Dependent Claim
21 words
The test strip of claim 2 wherein the aniline or phenol derivative is N-methylanthranilic acid, dimethylanthranilic acid, N-ethyl-N-(3'-sulphobenzene)-aniline or 2,3- xylenol.
Claim 5
Dependent Claim
25 words
The test strip of claim 2, wherein said chromophoric substrate is Tos-Gly-Pro-Arg-p-phenylenediamine, said aniline derivative is N-methylanthranilic acid and said oxidizing agent is potassium ferricyanide.
Claim 6
Dependent Claim
22 words
The test strip of claim 2, further comprising a third sample receiving carrier material positioned above or below said first carrier material.
Claim 7
Dependent Claim
30 words
The test strip of claim 5, further comprising a fiber fleece arranged between the third and first carrier material and separating said third and first carrier materials from each other.
Claim 8
Dependent Claim
30 words
The test strip of claim 5, further comprising a fiber fleece arranged between the first and second carrier material and separating said first and second carrier materials from each other.
Claim 9
Dependent Claim
24 words
The test strip of claim 1 useful in carrying out a Quick Test, comprising thromboplastin, a chromophoric substrate for thrombin and Ca 2+ ions.
Claim 10
Dependent Claim
22 words
The test strip of claim 1 useful in analysis of prothrombin, comprising Factor Xa, Factor V, Ca 2+ ions and a phospholipid.
Claim 11
Dependent Claim
32 words
The test strip of claim 1 useful in carrying out a partial thromboplastin time test, comprising a thrombin activator, a contact factor activator, a chromophoric substrate for thrombin, and Ca 2+ ions.
Claim 12
Dependent Claim
13 words
The test strip of claim 10, further comprising ellagic acid and a phospholipid.
Claim 13
Dependent Claim
56 words
The test strip of claim 1 useful in analysis of Factor X, comprising at least one factor involved in blood coagulation selected from the group consisting of venom obtained from Russell's viper and Factor X activating enzyme derived from Russell's viper venom, and further comprising Ca 2+ ions and a chromophoric substrate for factor Xa .
Claim 14
Dependent Claim
41 words
The test strip of claim 1 useful in analysis of Factor VIII, comprising Factor IXa, thrombin, Ca 2+ ions, a phospholipid and one member of the group consisting of a chromophoric substrate for Factor Xa and a chromophoric substrate for thrombin.
Claim 15
Dependent Claim
24 words
The test strip of claim 1 useful in analysis of Factor VII, comprising thromboplastin, Ca 2+ ions and a chromophoric substrate for Factor Xa.
Claim 16
Dependent Claim
46 words
The test strip of claim 1 useful in analysis of factor IX comprising an activated contact factor, Factor IXa, Ca 2+ ions and one of a chromophoric substrate for Factor IXa, or a combination of phospholipid, Factor VIII, thrombin and a chromophoric substrate for Factor Xa.
Claim 17
Dependent Claim
105 words
The test strip of claim 1 wherein said chromphoric substrate is a compound of the formula ##STR3## wherein A is the amino acid arginine or lysine, X is an N-terminal amino acid protective group, Y is a single bond or a chain of 1 to 3 amino acids, NR 1 R 2 is a group in the o- or p-position in which R 1 and R 2 , independently of one another, are hydrogen, alkyl containing up to 3 carbon atoms or nitro and R 3 is hydrogen, a carboxyl ester or carboxylamido group, halogen, nitro or an alkyl containing up to 3 carbon atoms.
Claim 18
Dependent Claim
14 words
The test strip of claim 16 wherein said chromophoric substrate is a Tos-Gly-Pro-Arg derivative.
Claim 19
Dependent Claim
47 words
The test strip of claim 16, further comprising a second carrier material which comprises an oxidizing agent and said first carrier material comprises an aniline or phenol derivative which forms a colored compound with the chromophore of the chromophoric substrate in the presence of said oxidizing agent.
Claim 20
Dependent Claim
12 words
The test strip of claim 1, wherein the carrier material is absorbent.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-10-21 | 1995-04-22 | 1995-10-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-10-21 | 1999-04-22 | 1999-10-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-10-21 | 2003-04-22 | 2003-10-22 | $7700.00 | Window Closed / Lapsed |