US5082780A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures
Assignee: Clinical Diagnostic Systems Inc
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Filing: 1989-09-12
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Granted: 1992-01-21
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Expires: 2009-09-12
Abstract
Abstract A covalent conjugate of an enzyme, such as peroxidase, glucose oxidase, alkaline phosphatase and beta-galactosidase, and an oligonucleotide is herein disclosed. This conjugate can be used as a probe in hybridization assays and in polymerase chain reaction procedures.
Inventors
- 👤 Harold C. Warren, III
- 👤 Fred T. Oakes
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 20:49
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C07; C07H; C07H21/00
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
103 words
A covalent conjugate of an enzyme and an oligonucleotide having the structure: ##STR22## wherein X--NH-- represents an enzyme having a reactive amino group from which a hydrogen atom has been removed, R 1 , R 2 , R 3 and R 4 are independently hydrogen, alkyl of 1 to 3 carbon atoms or hydroxyalkyl of 1 to 3 carbon atoms, m is a positive integer of 2 to 12, n is a positive integer of 1 to 50, and ##STR23## represents an oligonucleotide chain from which a hydroxy group has been removed from the terminal phosphate at the 3' or 5' end thereof.
Claim 2
Dependent Claim
103 words
A covalent conjugate of an enzyme and an oligonucleotide having the structure: ##STR22## wherein X--NH-- represents an enzyme having a reactive amino group from which a hydrogen atom has been removed, R 1 , R 2 , R 3 and R 4 are independently hydrogen, alkyl of 1 to 3 carbon atoms or hydroxyalkyl of 1 to 3 carbon atoms, m is a positive integer of 2 to 12, n is a positive integer of 1 to 50, and ##STR23## represents an oligonucleotide chain from which a hydroxy group has been removed from the terminal phosphate at the 3' or 5' end thereof.
Claim 3
Dependent Claim
22 words
The conjugate of claim 1 wherein said enzyme is peroxidase, and said oligonucleotide is attached to said conjugate at the 5' end.
Claim 4
Dependent Claim
32 words
The conjugate of claim 1 wherein R 1 , R 2 , R 3 and R 4 are independently hydrogen, methyl or hydroxymethyl, m is 2 and n is 1 to 15.
Claim 5
Dependent Claim
22 words
The conjugate of claim 1 wherein said enzyme is selected from the group consisting of peroxidase, glucose oxidase, alkaline phosphatase and β-galactosidase.
References Cited (Backward Citations)
US4510246A
Cited
US4536476A
Cited
US4711964A
Cited
WO1989002932A1
Cited
US4914210A
Cited
US4962029A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-01-20 | 1995-07-22 | 1996-01-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-01-20 | 1999-07-22 | 2000-01-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-01-20 | 2003-07-22 | 2004-01-21 | $7700.00 | Window Closed / Lapsed |