US5108564A
Expired / Lapsed
US
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Method and apparatus for amperometric diagnostic analysis
Assignee: Roche Diabetes Care Inc
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Filing: 1991-08-15
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Granted: 1992-04-28
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Expires: 2011-08-15
Abstract
Abstract The present invention relates to a novel method and apparatus for the amperometric determination of an analyte, and in particular, to an apparatus for amperometric analysis utilizing a novel disposable electroanalytical cell for the quantitative determination of biologically important compounds from body fluids.
Inventors
- 👤 Neil J. Szuminsky
- 👤 Joseph Jordan
- 👤 Paul A. Pottgen
- 👤 Jonathan L. Talbott
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 01:38
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
100 words
A method of measuring the amount of a selected compound in body fluids comprising, a) providing a measuring cell having at least a first and second electrode and said cell containing an oxidant and a buffer, b) placing a sample of fluid to be tested in said cell, c) reconstituting said oxidant and buffer with said sample fluid to generate a predetermined reaction, d) allowing said reaction to proceed substantially to completion, e) applying a potential across said electrodes and sample, and f) measuring the resulting Cottrell current to determine the concentration of said selected compound present in said sample.
Claim 2
Independent Claim
100 words
A method of measuring the amount of a selected compound in body fluids comprising, a) providing a measuring cell having at least a first and second electrode and said cell containing an oxidant and a buffer, b) placing a sample of fluid to be tested in said cell, c) reconstituting said oxidant and buffer with said sample fluid to generate a predetermined reaction, d) allowing said reaction to proceed substantially to completion, e) applying a potential across said electrodes and sample, and f) measuring the resulting Cottrell current to determine the concentration of said selected compound present in said sample.
Claim 3
Dependent Claim
28 words
A method as set forth in claim 1, wherein the compound is selected from the group consisting of glucose, cholesterol, TSH, T4, hormones, antiarrhythmics, antiepileptics and nontherapeutic drugs.
Claim 4
Dependent Claim
30 words
A method as set forth in claim 1, wherein the oxidant is a material selected from the group consisting of benzoquinone, ferricyanide, ferricinium, Cobalt (III) orthophenantroline and Cobalt (III) dipyridyl.
Claim 5
Dependent Claim
25 words
The method as set forth in claim 1 including providing as said first electrode a working electrode and as said second electrode a reference electrode.
Claim 6
Dependent Claim
22 words
The method of claim 1 including also providing in said cell and enzyme as a catalyst and said enzyme is an oxidoreductase.
Claim 7
Dependent Claim
25 words
The method of claim 1 including selecting said buffer from the group consisting of phosphate, TRIS, MOPS, MES, HEPES, Tricine, Bicine, ACES, CAPS and TAPS.
Claim 8
Independent Claim
99 words
A method for measuring the amount of glucose in blood, comprising a) providing a measuring cell having at least a first and second electrode and said cell containing an oxidant, a buffer and an enzyme, b) placing a blood sample to be tested in said cell, c) reconstituting said oxidant, buffer and enzyme with said blood sample to generate a predetermined reaction, d) essentially immediately applying a potential across said electrodes and blood sample, and e) measuring the resultant Cottrell current when the reaction has proceeded to completion to determine the concentration of said glucose present in blood sample.
Claim 9
Independent Claim
99 words
A method for measuring the amount of glucose in blood, comprising a) providing a measuring cell having at least a first and second electrode and said cell containing an oxidant, a buffer and an enzyme, b) placing a blood sample to be tested in said cell, c) reconstituting said oxidant, buffer and enzyme with said blood sample to generate a predetermined reaction, d) essentially immediately applying a potential across said electrodes and blood sample, and e) measuring the resultant Cottrell current when the reaction has proceeded to completion to determine the concentration of said glucose present in blood sample.
Claim 10
Dependent Claim
24 words
The method of claim 7 including selecting said oxidant from the group consisting of benzoquinone, ferricyanide, ferricinium, Cobalt (III) orthophenantroline, and Cobalt (III) dipyridyl.
Claim 11
Dependent Claim
21 words
The method of claim 7 including providing as said first electrode a working electrode and said second electrode a reference electrode.
Claim 12
Dependent Claim
12 words
The method of claim 7 including adding as said enzyme, glucose oxydase.
Claim 13
Dependent Claim
50 words
The method of claim 1 wherein in step b) said placing of the blood sample to be tested in the cell generates a current and initiates a timing sequence, and wherein the reaction of step d) is allowed to proceed with an open circuit between said first and second electrode.
References Cited (Backward Citations)
US4654197A
Cited
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