US5114847A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the production of permanently culturable animal and human cell lines and the use thereof
Assignee: Roche Diagnostics GmbH
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Filing: 1990-09-25
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Granted: 1992-05-19
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Expires: 2010-09-25
Abstract
Abstract A method for obtaining permanently culturable human and animal cell lines, as well as uses for these cell lines, are disclosed. The method involves fusing cells of a non-immortal cell line with cytoplasts or cytoplasma fractions of "immortal", transformed cells such as immortal myeloma cells, ascites-tumor cells or Epstein Barr Virus infected cells. Once fusion takes place the product is a permanently culturable variant of the previously normal cell line.
Inventors
- 👤 Herbert Jungfer
- 👤 Heinrich Barchet
- 👤 Winfried Albert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 23:13
CPC Classifications
C; C07; C07K; C07K16/00; C07K14/00; C07K14/435; C07K14/52; C12; C12N; C12N15/00
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
59 words
A process for obtaining animal and human cells which are permanently culturable in vitro, comprising the steps of: (a) fusing normal animal or human cells with cytoplasts or cytoplasm fractions which are incapable of multiplying and which are obtained from transformed cells; and (b) cultivating the fused product of step (a) in non-selective medium to obtain permanently culturable cells.
Claim 2
Dependent Claim
59 words
A process for obtaining animal and human cells which are permanently culturable in vitro, comprising the steps of: (a) fusing normal animal or human cells with cytoplasts or cytoplasm fractions which are incapable of multiplying and which are obtained from transformed cells; and (b) cultivating the fused product of step (a) in non-selective medium to obtain permanently culturable cells.
Claim 3
Dependent Claim
19 words
A process according to claim 1, wherein fusion takes place in the presence of polyethylene glycol or Sendai virus.
Claim 4
Dependent Claim
21 words
A process according to claim 1, wherein said cytoplasts or cytoplasm fractions are obtained from transformed cells treated with cytochalasin B.
Claim 5
Dependent Claim
22 words
A process according to claim 1, wherein said cytoplasts or cytoplasm fractions are obtained by lysis or mechanical digestion of transformed cells.
Claim 6
Dependent Claim
28 words
A process according to claim 1, wherein said cytoplasts or cytoplasm fractions are cytoplasts or cytoplasm fractions of myeloma cells, ascites tumor cells or Epstein-Barr virus infected cells.
Claim 7
Dependent Claim
35 words
A method of obtaining a cell product from the permanently culturable cells of claim 1, comprising culturing said cells under conditions favoring production of a cell product therefrom, and separating said product from said cells.
Claim 8
Dependent Claim
25 words
A permanently culturable cell line comprising cells derived from the fusion of normal human or animal cells with cytoplasts or cytoplasm fractions from transformed cells.
Claim 9
Dependent Claim
25 words
A permanently culturable cell line comprising cells derived from the fusion of normal human or animal cells with cytoplasts or cytoplasm fractions from transformed cells.
References Cited (Backward Citations)
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Cited
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-05-19 | 1995-11-18 | 1996-05-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-05-19 | 1999-11-18 | 2000-05-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-05-19 | 2003-11-18 | 2004-05-19 | $7700.00 | Window Closed / Lapsed |