US5124267A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the determination of small substance quantities of medicines, of endogenous or other chemical compounds in biological material
Assignee: Bayer Pharma AG
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Filing: 1986-01-10
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Granted: 1992-06-23
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Expires: 2006-01-10
Abstract
Abstract The invention relates to a process for the determination of small substance quantities of medicines, of endogenous or other chemical compounds in biological material, characterized by either binding the compound to be determined and its deuterated analog to an antibody present in excess, which antibody is coupled to a stationary phase of a polymeric material, and separating the two compounds from the antibody and measuring by mass spectrometry the proportion of deuterated compound/not deuterated compound or, respectively, cross-reacting compound; or, in case a suitable deuterated or cross-reacting compound is not available, utilizing as the internal standard also a chemically similar compound which, however, is added after the antibody-mediated extraction, and inserting a separating process before mass spectrometry.
Inventors
- 👤 Michael Humpel
- 👤 Werner Krause
- 👤 Paul-Eberhard Schulze
- 👤 Bob Nieuweboer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 23:24
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; C; C07; C07C; C07C59/00; C07C59/40; C07C59/42; C07C59/46; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/817; Y10T; Y10T436/00; Y10T436/24
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
92 words
A method for determining a small amount of a chemical compound in a sample, comprising adding to the sample a known amount of a stable isotopic analog of said compound, binding said analog and said compound to an excess of antibody on a stationary phase for both said analog and compound, releasing said analog and compound from the antibody, and determining by mass spectrometric analysis the ratio of the released amount of said isotopic analogue to the released amount of said chemical compound wherefrom the amount of said chemical compound is calculable.
Claim 2
Independent Claim
92 words
A method for determining a small amount of a chemical compound in a sample, comprising adding to the sample a known amount of a stable isotopic analog of said compound, binding said analog and said compound to an excess of antibody on a stationary phase for both said analog and compound, releasing said analog and compound from the antibody, and determining by mass spectrometric analysis the ratio of the released amount of said isotopic analogue to the released amount of said chemical compound wherefrom the amount of said chemical compound is calculable.
Claim 3
Dependent Claim
17 words
A method of claim 1, wherein said isotopic analog is a deuterated analog of said chemical compound.
Claim 4
Dependent Claim
14 words
A method of claim 1, wherein said antibody is bound to a stationary phase.
Claim 5
Dependent Claim
26 words
A method of claim 3, wherein, prior to said mass spectrometry step, said isotopic analog and chemical compound are separated from each other by gas chromatography.
Claim 6
Dependent Claim
22 words
A method of claim 1, wherein said chemical compound is a medicinal substance or an endogenous chemical substance in a biological sample.
Claim 7
Dependent Claim
15 words
A method of claim 5, wherein said medicinal substance is Iloprost, estradiol or 11-Nor-9-tetrahydrocannabinol-9-carboxylic acid.
Claim 8
Independent Claim
102 words
A method for determining a small amount of a compound in a sample, comprising adding to the sample a known amount of a compound which cross-reacts with an antibody to said chemical compound, binding said chemical compound and said cross-reacting compound to an excess of said antibody on a stationary phase, releasing said chemical and cross-reaction compound from the antibody, separating the chemical and cross-reacting compounds from each other, and determining by mass spectrometric analysis the ratio of the released amount of said cross-reacting compound to the released amount of said chemical compound wherefrom the amount of said chemical compound is calculable.
Claim 9
Independent Claim
102 words
A method for determining a small amount of a compound in a sample, comprising adding to the sample a known amount of a compound which cross-reacts with an antibody to said chemical compound, binding said chemical compound and said cross-reacting compound to an excess of said antibody on a stationary phase, releasing said chemical and cross-reaction compound from the antibody, separating the chemical and cross-reacting compounds from each other, and determining by mass spectrometric analysis the ratio of the released amount of said cross-reacting compound to the released amount of said chemical compound wherefrom the amount of said chemical compound is calculable.
Claim 10
Independent Claim
125 words
A method for determining a small amount of a chemical compound in a sample, comprising extracting said compound from said sample by binding said compound to an excess of antibody on a stationary phase therefor and separating said compound from said antibody, adding to said separated compound a known amount of a chemical analog of said compound, which is not merely a deuterated analog thereof, of sufficient structural similarity to provide an accurate standard for said compound, thereafter separating said analogous compound from said compound and analyzing each of said separated compounds by mass spectrometry in order to determine a ratio of the amount of said chemical compound to the amount of said added analogous compound, wherefrom the amount of said chemical compound is calculable.
Claim 11
Independent Claim
125 words
A method for determining a small amount of a chemical compound in a sample, comprising extracting said compound from said sample by binding said compound to an excess of antibody on a stationary phase therefor and separating said compound from said antibody, adding to said separated compound a known amount of a chemical analog of said compound, which is not merely a deuterated analog thereof, of sufficient structural similarity to provide an accurate standard for said compound, thereafter separating said analogous compound from said compound and analyzing each of said separated compounds by mass spectrometry in order to determine a ratio of the amount of said chemical compound to the amount of said added analogous compound, wherefrom the amount of said chemical compound is calculable.
Claim 12
Independent Claim
143 words
A method for determining a small amount of a chemical compound in a sample, comprising determining by mass spectrometric analysis the ratio of the amount of a stable isotopic or cross-reacting analog of said compound in said sample to the amount of said chemical compound in said sample in order to correlate therewith a determination of the amount of said chemical compound, said stable isotopic or cross-reacting analog having been added to said sample in a known amount and both said stable isotopic or cross-reacting analog and said chemical compound having been separated from said sample by binding to an excess of antibody on a stationary phase for both said isotopic or cross-reacting analog and said chemical compound and thereafter having been released from the antibody prior to said ratio determination step, said cross-reacting analog being a compound which cross-reacts with said antibody.
Claim 13
Independent Claim
143 words
A method for determining a small amount of a chemical compound in a sample, comprising determining by mass spectrometric analysis the ratio of the amount of a stable isotopic or cross-reacting analog of said compound in said sample to the amount of said chemical compound in said sample in order to correlate therewith a determination of the amount of said chemical compound, said stable isotopic or cross-reacting analog having been added to said sample in a known amount and both said stable isotopic or cross-reacting analog and said chemical compound having been separated from said sample by binding to an excess of antibody on a stationary phase for both said isotopic or cross-reacting analog and said chemical compound and thereafter having been released from the antibody prior to said ratio determination step, said cross-reacting analog being a compound which cross-reacts with said antibody.
References Cited (Backward Citations)
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