US5132208A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Test carrier for the analysis of a sample liquid
Assignee: Roche Diagnostics GmbH
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Filing: 1988-05-03
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Granted: 1992-07-21
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Expires: 2008-05-03
Abstract
Abstract The present invention provides a test carrier for the analysis of a sample liquid and especially of a body fluid, having a porous test layer (8, 13) which contains a solid component (9, 17), wherein the solid component (9, 17) is coated with a protein which is insoluble in the sample liquid under the test conditions. A process for the production of this test layer is also disclosed, wherein the protein is dissolved in a solvent under conditions under which the solubility of the protein is sufficiently high in order to dissolve a certain minimum amount of the protein, the solid component of the test layer is contracted with the solution and the solubility is reduced to such an extent that the component is coated by the precipitating protein.
Inventors
- 👤 Helmut Freitag
- 👤 Hans-Erich Wilk
- 👤 Anselm Rothe
- 👤 Heino Eikmeier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 20:26
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
126 words
A layered test carrier device for analysis of an analyte in a liquid sample, comprising: (a) a porous test layer having a 3-dimensional open pored structure and comprising a solid component coated with a protein which is insoluble under conditions of use and which also prevents undesired binding of a macromolecular substance which (i) might be present in the liquid sample and is to be determined or (ii) is present in said device and reacts with the analyte to be determined, and (b) a reagent incorporated into said device which reacts with said analyte to produce a detectable signal representative of said analysis, said reagent positioned at a point in said device contacted by said liquid sample after said liquid sample contacts said porous test layer.
Claim 2
Dependent Claim
126 words
A layered test carrier device for analysis of an analyte in a liquid sample, comprising: (a) a porous test layer having a 3-dimensional open pored structure and comprising a solid component coated with a protein which is insoluble under conditions of use and which also prevents undesired binding of a macromolecular substance which (i) might be present in the liquid sample and is to be determined or (ii) is present in said device and reacts with the analyte to be determined, and (b) a reagent incorporated into said device which reacts with said analyte to produce a detectable signal representative of said analysis, said reagent positioned at a point in said device contacted by said liquid sample after said liquid sample contacts said porous test layer.
Claim 3
Dependent Claim
12 words
Layered test carrier device of claim 1, wherein said protein is casein.
Claim 4
Dependent Claim
25 words
Layered test carrier device of claim 1, wherein said reagent comprises a biochemical substance which is contained in said porous test layer in elutable form.
Claim 5
Dependent Claim
13 words
Layered test carrier device of claim 1, wherein said reagent comprises an enzyme.
Claim 6
Dependent Claim
12 words
Layered test carrier device of claim 4, wherein said enzyme is galactosidase.
Claim 7
Dependent Claim
14 words
Layered test carrier device of claim 1, wherein said reagent is a galactosidase conjugate.
Claim 8
Dependent Claim
16 words
Layered test carrier device of claim 1, wherein said porous test layer contains a textile structure.
Claim 9
Dependent Claim
16 words
Layered test carrier device of claim 1, wherein said porous test layer comprises polyester or polyamide.
Claim 10
Dependent Claim
13 words
Layered test carrier device of claim 1, wherein said solid component comprises particles.
Claim 11
Dependent Claim
14 words
Layered test carrier device of claim 9, wherein said particles consist of inorganic material.
Claim 12
Dependent Claim
14 words
Layered test carrier device of claim 10, wherein said inorganic material is silicon dioxide.
Claim 13
Dependent Claim
38 words
Method for determining an analyte in a liquid sample comprising contacting said liquid sample to the layered test carrier device of claim 1 and evaluating reaction between said analyte and said reagents as an indication of said analyte.
Claim 14
Dependent Claim
38 words
Method for determining an analyte in a liquid sample comprising contacting said liquid sample to the layered test carrier device of claim 2 and evaluating reaction between said analyte and said reagents as an indication of said analyte.
Claim 15
Dependent Claim
38 words
Method for determining an analyte in a liquid sample comprising contacting said liquid sample to the layered test carrier device of claim 3 and evaluating reaction between said analyte and said reagents as an indication of said analyte.
Claim 16
Dependent Claim
11 words
Method of claim 14, wherein said biochemical substance comprises an enzyme.
Claim 17
Dependent Claim
17 words
Method of claim 14, wherein said biochemical substance is a conjugate of an immunoreactant and an enzyme.
Claim 18
Dependent Claim
9 words
Method of claim 15, wherein said enzyme is galactosidase.
Claim 19
Dependent Claim
9 words
Method of claim 16, wherein said enzyme is galactosidase.
Claim 20
Dependent Claim
60 words
Method for determining an analyte in a liquid sample comprising contacting said liquid sample to the layered test carrier device of claim 1, under conditions permitting flow of said liquid sample containing said analyte and said macromolecular test substances through said porous test layer and said evaluating reaction between said analyte and said reagents as an indication of said analyte.
References Cited (Backward Citations)
US4312834A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-07-21 | 1996-01-20 | 1996-07-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-07-21 | 2000-01-20 | 2000-07-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-07-21 | 2004-01-20 | 2004-07-21 | $7700.00 | Window Closed / Lapsed |