US5143838A
Expired / Lapsed
US
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Method of producing thrombin from factor ii using calcium ions for the conversion on an anion exchanger
Assignee: Omrix Biopharmaceuticals SA
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Filing: 1989-12-20
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Granted: 1992-09-01
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Expires: 2009-12-20
Abstract
Abstract A method of producing thrombin from Factor II (prothrombin) comprising the steps of: a) applying a solution of citrated plasma or citrated plasma fraction containing Factor II onto an equilibrated anion exchanger to bind Factor II thereto; b) applying a solution containing calcium ions to the exchanger to convert the Factor II to thrombin, and c) selectively eluting the thrombin from the carrier.
Inventors
- 👤 Michael Kraus
- 👤 Wolfgang Moller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 13:39
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6429; C12Y; C12Y304/00; C12Y304/21; C12Y304/21005
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
62 words
A method of producing thrombin from Factor II comprising the steps of: a) applying a solution of citrated plasma or citrated plasma fraction containing Factor II onto an equilibrated anion exchanger to bind Factor II thereto; b) applying a solution containing calcium ions to the exchanger to convert the Factor II to thrombin, and c) selectively eluting the thrombin from the carrier.
Claim 2
Independent Claim
62 words
A method of producing thrombin from Factor II comprising the steps of: a) applying a solution of citrated plasma or citrated plasma fraction containing Factor II onto an equilibrated anion exchanger to bind Factor II thereto; b) applying a solution containing calcium ions to the exchanger to convert the Factor II to thrombin, and c) selectively eluting the thrombin from the carrier.
Claim 3
Dependent Claim
39 words
The method according to claim 1, wherein the anion exchange matrix carries DEAE or QAE groups and is (a) a copolymer of glycidyl methacrylate, pentaerythritol dimethacrylate or polyvinyl alcohol, (b) a copolymer of N-acryloyl-2-amino-2-hydroxymethyl-1,3-propanediol, or (c) a carbohydrate polymer.
Claim 4
Dependent Claim
13 words
The method according to claim 1, wherein the anion exchanger has 2-hydroxy-aminopropyl groups.
Claim 5
Dependent Claim
23 words
The method according to claim 3, wherein the 2-hydroxy-aminopropyl groups are present on a copolymer of glycidyl methacrylate, pentaerythritol dimethacrylate, and polyvinyl alcohol.
Claim 6
Dependent Claim
16 words
The method according to claim 1, wherein the concentration of the calcium ions is 20-30 mM.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-01 | 1996-03-02 | 1996-09-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-01 | 2000-03-02 | 2000-09-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-01 | 2004-03-02 | 2004-09-01 | $7700.00 | Window Closed / Lapsed |