US5149633A
Expired / Lapsed
US
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Process and reagent for the specific determination of fructosamine content in blood and samples obtained from blood
Assignee: Roche Diagnostics GmbH
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Filing: 1988-09-26
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Granted: 1992-09-22
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Expires: 2008-09-26
Abstract
Abstract Process for the specific determination of the serum fructosamine content in blood or in samples obtained from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, in which before the color reaction sample components with a non-specific reducing action and/or causing turbidity are removed and subsequently the color reagent is added at a pH value of from 10 to 12. The sample components are removed by treatment at approximately neutral pH value with a reagent composition comprising at least one enzymatic oxidation agent, optionally together with peroxidase and/or catalase and/or lipase, as well as with at least one SH group-blocking substance. A kit for the specific determination of the serum fructosamine content in blood or samples obtained from blood, comprises said reagent composition, a rebuffering reagent with a buffer which has an alkaline pH value and a color reagent for the detection of fructosamine.
Inventors
- 👤 Bernd Vogt
- 👤 Liselotte Schellong
- 👤 Joachim Siedel
- 👤 Joachim Ziegenhorn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 10:23
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/34; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/66; G01N33/96; C12Q2326/00; C12Q2326/90; C12Q2326/92; Y; Y10; Y10S; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444; Y10T436/17; Y10T436/173845; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
150 words
In a process for the specific determination of the serum fructosamine content on a blood sample or in sample obtained from blood by reaction with a tetrazolium salt at an alkaline pH and measurement of the resultant color change, the improvement comprising: (i) removing components which cause non-specific reduction of the tetrazolium salt or which cause turbidity by treating the sample with a reagent composition comprising at least one oxidoreductase and at least one SH group blocking agent in an amount sufficient to prevent non-specific reduction of the tetrazolium salt from 1 to 15 minutes, the pH of said composition being from 6 to 9, (ii) bringing the sample to a pH of from 10 to 12 with a rebuffering reagent prior to or simultaneously with the reaction with the tetrazolium salt, and (iii) determining color formation or color change from 1 to 15 minutes after adding said rebuffering reagent.
Claim 2
Dependent Claim
150 words
In a process for the specific determination of the serum fructosamine content on a blood sample or in sample obtained from blood by reaction with a tetrazolium salt at an alkaline pH and measurement of the resultant color change, the improvement comprising: (i) removing components which cause non-specific reduction of the tetrazolium salt or which cause turbidity by treating the sample with a reagent composition comprising at least one oxidoreductase and at least one SH group blocking agent in an amount sufficient to prevent non-specific reduction of the tetrazolium salt from 1 to 15 minutes, the pH of said composition being from 6 to 9, (ii) bringing the sample to a pH of from 10 to 12 with a rebuffering reagent prior to or simultaneously with the reaction with the tetrazolium salt, and (iii) determining color formation or color change from 1 to 15 minutes after adding said rebuffering reagent.
Claim 3
Dependent Claim
21 words
A process according to claim 1 in which the rebuffering reagent is added to the sample simultaneously with the tetrazolium salt.
Claim 4
Dependent Claim
31 words
A process according to claim 1 in which the pH of the reagent composition is from 7.0 to 8.5 and the pH of the rebuffering reagent is from 10.5 to 12.5.
Claim 5
Dependent Claim
31 words
A process according to claim 1 in which the pH of the reagent composition is from 7.5 to 8.0 and the pH of the rebuffering reagent is from 10.7 to 12.2.
Claim 6
Dependent Claim
19 words
A process according to claim 1 in which the pH of the rebuffered sample is from 10.3 to 10.6.
Claim 7
Dependent Claim
17 words
A process according to claim 1 in which the oxidureductase is ascorbate oxidase, bilirubin oxidase or uricase.
Claim 8
Dependent Claim
19 words
A process according to claim 1 in which the SH group blocking agent is iodoacetamide, iodoacetate, N-ethylmaleinimide or p-hydroxymercuribenzoate.
Claim 9
Dependent Claim
15 words
A process according to claim 1 in which the SH group blocking agent is iodoacetamide.
Claim 10
Dependent Claim
16 words
A process according to claim 1 in which the reagent composition additionally comprises peroxidase or catalase.
Claim 11
Dependent Claim
17 words
A process according to claim 1 in which the reagent composition additionally comprises at least one detergent.
Claim 12
Dependent Claim
16 words
A process according to claim 10 in which the detergent is a non-ionic or anionic detergent.
Claim 13
Dependent Claim
21 words
A process according to claim 1, in which the reagent further comprises a detergent and a salt of a strong acid.
Claim 14
Dependent Claim
37 words
A process according to claim 12 in which the detergent is an ionic or non-ionic detergent and the salt of a strong acid is an alkali metal or alkaline earth metal salt of hydrochloric or sulfuric acid.
Claim 15
Dependent Claim
16 words
A process according to claim 13 in which the salt is potassium chloride or sodium chloride.
Claim 16
Dependent Claim
14 words
A process according to claim 1 in which the reagent composition additionally comprises lipase.
Claim 17
Dependent Claim
29 words
A process according to claim 1 in which the reagent composition comprises uricase, bilirubin oxidase, ascorbate oxidase, lipase, peroxidase or catalase and at least one SH group blocking agent.
Claim 18
Dependent Claim
15 words
A process according to claim 16 in which the SH group blocking substance is iodoacetamide.
Claim 19
Dependent Claim
23 words
A process according to claim 17 n which the reagent composition additionally comprises an ionic or non-ionic detergent and an alkali metal chloride.
Claim 20
Dependent Claim
81 words
A kit for the specific determination of the serum fructosamine content in a blood sample or in a sample obtained from blood which comprises (1) a tetrazolium salt, (2) a reagent composition comprising at least one oxidoreductase and at least one SH group blocking agent in an amount sufficient to prevent non-specific reduction of the tetrazolium salt, the pH of said reagent composition being from 6 to 9, and (3) a rebuffering reagent having a pH of from 10.5 to 12.5.
Claim 21
Dependent Claim
81 words
A kit for the specific determination of the serum fructosamine content in a blood sample or in a sample obtained from blood which comprises (1) a tetrazolium salt, (2) a reagent composition comprising at least one oxidoreductase and at least one SH group blocking agent in an amount sufficient to prevent non-specific reduction of the tetrazolium salt, the pH of said reagent composition being from 6 to 9, and (3) a rebuffering reagent having a pH of from 10.5 to 12.5.
Claim 22
Dependent Claim
17 words
A kit according to claim 19 in which said tetrazolium salt is dissolved in the rebuffering reagent.
Claim 23
Dependent Claim
31 words
A kit according to claim 19 in which the pH of the reagent composition is from 7 to 8.5 and the pH of the rebuffering reagent is from 10.7 to 12.2.
Claim 24
Dependent Claim
18 words
A kit according to claim 19 in which the reagent composition additionally comprises peroxidase or catalase or lipase.
Claim 25
Dependent Claim
17 words
A kit according to claim 19 in which the oxidoreductase is ascorbate oxidase, bilirubin oxidase or uricase.
Claim 26
Dependent Claim
20 words
A kit according to claim 19 in which the SH group blocking agent is iodoacetamide, iodoacetate, N-ethylmaleinimide or p-hydroxyme ribenzoate.
Claim 27
Dependent Claim
14 words
A kit according to claim 19 in which the SH blocking agent is iodoacetamide.
Claim 28
Dependent Claim
31 words
A kit according to claim 22 which additionally comprises a cationic, anionic or non-ionic detergent and additionally comprises an alkali metal or alkaline earth metal salt of hydrochloric or sulfuric acid.
Claim 29
Dependent Claim
23 words
A kit according to claim 19 in which the reagent composition comprises peroxidase, uricase, lipase, iodoacetamide, a non-ionic detergent and an anionic detergent.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-22 | 1996-03-23 | 1996-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-22 | 2000-03-23 | 2000-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-22 | 2004-03-23 | 2004-09-22 | $7700.00 | Window Closed / Lapsed |