US5171689A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Solid state bio-sensor
Assignee: Panasonic Holdings Corp
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Filing: 1989-04-18
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Granted: 1992-12-15
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Expires: 2009-04-18
Abstract
Abstract As the conventional simple bio-sensors for measuring the particular component in living bodies, there are those in which a change in dye caused by enzyme reaction is detected optically, but such bio-sensors had a problem that precision is low owing to disturbance of the color of liquid samples. With bio-sensors using an enzyme electrode, the precision was high, but operation of measurement was troublesome. The present invention made it possible to detect the concentration of the particular component electromechanically with rapidity, simplicity and high precision by merely putting a porous substrate containing at least enzyme on the electrode system and impregnating the body with a liquid sample.
Inventors
- 👤 Mariko Kawaguri
- 👤 Shiro Nankai
- 👤 Takashi Iijima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 14:24
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
86 words
A bio-sensor for measuring a substrate concentration of a liquid sample, comprising: at least a working electrode and a counter electrode, an insulating substrate plate which supports and insulates the working electrode and the counter electrode from one another, and a porous plate fixed on the insulating substrate plate, wherein said porous plate carries at least an oxidoreductase and a buffer, the oxidoreductase and the buffer are in a dry state, and wherein the bio-sensor is constructed so as not to contain a liquid containing chamber.
Claim 2
Dependent Claim
86 words
A bio-sensor for measuring a substrate concentration of a liquid sample, comprising: at least a working electrode and a counter electrode, an insulating substrate plate which supports and insulates the working electrode and the counter electrode from one another, and a porous plate fixed on the insulating substrate plate, wherein said porous plate carries at least an oxidoreductase and a buffer, the oxidoreductase and the buffer are in a dry state, and wherein the bio-sensor is constructed so as not to contain a liquid containing chamber.
Claim 3
Dependent Claim
12 words
The bio-sensor according to claim 1, wherein the buffer is a phosphate.
Claim 4
Dependent Claim
20 words
The bio-sensor according to claim 1, wherein an electron acceptor is carried on the porous substrate in a dry state.
Claim 5
Dependent Claim
11 words
The bio-sensor according to claim 1 further comprises an oxidizing dye.
Claim 6
Dependent Claim
30 words
The bio-sensor according to claim 4, wherein the oxidizing dye is a member selected from the group consisting of potassium ferricyanide, p-benzoquinone, 2,6-dichlorophenolindophenol, methylene blue, phenazine methosulfate and potassium beta-naphtoquinone-4-sulfonate.
Claim 7
Dependent Claim
27 words
The bio-sensor according to claim 1, wherein the oxidoreductase is a member selected from the group consisting of glucose oxidase, alcohol oxidase, xanthine oxidase and cholesterol oxidase.
Claim 8
Dependent Claim
12 words
The bio-sensor according to claim 1, wherein the oxidoreductase is glucose oxidase.
Claim 9
Dependent Claim
12 words
The bio-sensor according to claim 1, wherein the working electrode comprises platinum.
Claim 10
Dependent Claim
20 words
The bio-sensor according to claim 8, wherein the porous plate has a pore diameter of less than 3 micron meters.
Claim 11
Dependent Claim
11 words
The bio-sensor according to claim 1, further comprises a reference electrode.
Claim 12
Dependent Claim
15 words
The bio-sensor according to claim 1, wherein the porous plate is a hydrophilic porous membrane.
Claim 13
Dependent Claim
27 words
The bio-sensor according to claim 1, further comprising a filter having a pore size of less than 3 micron meters positioned and arranged on the porous plate.
Claim 14
Dependent Claim
14 words
The bio-sensor according to claim 12, wherein an anticoagulant is carried on the filter.
Claim 15
Dependent Claim
12 words
The bio-sensor according to claim 12, which further comprises a reference electrode.
Claim 16
Dependent Claim
15 words
The bio-sensor according to claim 1, wherein the counter electrode comprises platinum or silver/silver chloride.
Claim 17
Dependent Claim
21 words
The bio-sensor according to claim 1, wherein the pore size of the porous plate is between 1000 Angstroms and 1 mm.
Claim 18
Dependent Claim
107 words
A bio-sensor for measuring a substrate concentration of a liquid sample, which comprises: at least a working electrode and a counter electrode, an insulating substrate plate which supports and insulates the working electrode and the counter electrode from one another, and a porous plate fixed on the insulating substrate plate, wherein said porous plate carries at least an oxidoreductase and a buffer, the oxidoreductase and the buffer are in a dry state and contacts the electrodes without an intervening electrolyte layer between (i) the oxidoreductase and the buffer and (ii) the electrodes, and wherein the bio-sensor is constructed so as not to contain a liquid containing chamber.
Claim 19
Dependent Claim
107 words
A bio-sensor for measuring a substrate concentration of a liquid sample, which comprises: at least a working electrode and a counter electrode, an insulating substrate plate which supports and insulates the working electrode and the counter electrode from one another, and a porous plate fixed on the insulating substrate plate, wherein said porous plate carries at least an oxidoreductase and a buffer, the oxidoreductase and the buffer are in a dry state and contacts the electrodes without an intervening electrolyte layer between (i) the oxidoreductase and the buffer and (ii) the electrodes, and wherein the bio-sensor is constructed so as not to contain a liquid containing chamber.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-12-15 | 1996-06-15 | 1996-12-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-12-15 | 2000-06-15 | 2000-12-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-12-15 | 2004-06-15 | 2004-12-15 | $7700.00 | Window Closed / Lapsed |