US5180809A
Expired / Lapsed
US
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Adhesion receptor for laminin and its use
Assignee: Sanford Burnham Prebys Medical Discovery Institute
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Filing: 1989-05-25
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Granted: 1993-01-19
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Expires: 2009-05-25
Abstract
Abstract An adhesion receptor for laminin is provided. The receptor is isolated from cell or tissue extracts and fractionated on an affinity column composed of cell attachment-promoting fragments of laminin coupled to Sepharose™ in the presence of divalent cations. This receptor can be used to prepare specific antibodies for the analysis of the amount of laminin receptor expressed by cells and has other applications in cellular and tumor biology.
Inventors
- 👤 Erkki I. Ruoslahti
- 👤 Eva Engvall
- 👤 Kurt R. Gehlsen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 04:42
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/2839; C07K14/00; C07K14/435; C07K14/705; C07K14/70546; Y; Y10; Y10S; Y10S530/00; Y10S530/81
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
42 words
A substantially pure active mammalian laminin adhesion receptor, characterized by comprising two subunits, one of which is α 3 , the α 3 subunit further comprising two disulfide linked polypeptides, said receptor selectively binding with laminin or cell attachment-promoting fragments of laminin.
Claim 2
Dependent Claim
42 words
A substantially pure active mammalian laminin adhesion receptor, characterized by comprising two subunits, one of which is α 3 , the α 3 subunit further comprising two disulfide linked polypeptides, said receptor selectively binding with laminin or cell attachment-promoting fragments of laminin.
Claim 3
Dependent Claim
16 words
The receptor of claim 1, wherein said mammalian laminin adhesion receptor is derived from a rat.
Claim 4
Dependent Claim
16 words
The receptor of claim 1, wherein said mammalian laminin adhesion receptor is derived from a human.
Claim 5
Dependent Claim
26 words
The receptor of claim 1, wherein said receptor can be eluted from said selective binding with laminin or cell attachment-promoting fragments of laminin with ethylenediaminotetraacetic acid.
Claim 6
Dependent Claim
14 words
A composition of matter comprising the receptor of claim 1 incorporated into lipid vesicles.
Claim 7
Dependent Claim
14 words
A composition of matter comprising the receptor of claim 1 coated onto a substrate.
Claim 8
Dependent Claim
56 words
A composition of matter comprising fragments of a substantially pure active mammalian laminin adhesion receptor which retain ligand binding activity, said receptor characterized by comprising two subunits, one of which is α 3 , the α 3 subunit further comprising two disulfide liked polypeptides, said receptor selectively binding with laminin or cell attachment-promoting fragments of laminin.
Claim 9
Dependent Claim
56 words
A composition of matter comprising fragments of a substantially pure active mammalian laminin adhesion receptor which retain ligand binding activity, said receptor characterized by comprising two subunits, one of which is α 3 , the α 3 subunit further comprising two disulfide liked polypeptides, said receptor selectively binding with laminin or cell attachment-promoting fragments of laminin.
Claim 10
Dependent Claim
95 words
Laminin cell surface receptors isolated by the method comprising: a. running a mammalian cell extract preparation through an affinity column comprising the cell attachment-promoting portion of laminin coupled to a matrix, wherein said cell extract preparation is run through said column in the presence of divalent cation so as to achieve binding between the laminin cell surface receptors to be isolated and laminin cell attachment site; b. eluting said column with a solution containing a substance capable of selectively eluting said laminin cell surface receptor; and c. collecting said laminin cell surface receptors eluted thereby.
Claim 11
Dependent Claim
95 words
Laminin cell surface receptors isolated by the method comprising: a. running a mammalian cell extract preparation through an affinity column comprising the cell attachment-promoting portion of laminin coupled to a matrix, wherein said cell extract preparation is run through said column in the presence of divalent cation so as to achieve binding between the laminin cell surface receptors to be isolated and laminin cell attachment site; b. eluting said column with a solution containing a substance capable of selectively eluting said laminin cell surface receptor; and c. collecting said laminin cell surface receptors eluted thereby.
References Cited (Backward Citations)
US4565789A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-01-19 | 1996-07-20 | 1997-01-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-01-19 | 2000-07-20 | 2001-01-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-01-19 | 2004-07-20 | 2005-01-19 | $7700.00 | Window Closed / Lapsed |