US5187368A
Expired / Lapsed
US
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Detection method for liquids using near infrared spectra
Assignee: KENTUCKY THE, University of, Trustees of
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Filing: 1990-09-12
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Granted: 1993-02-16
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Expires: 2010-09-12
Abstract
Abstract A method for detecting the presence or absence of microorganisms in a liquid test sample is provided without need for withdrawing an aliquot or destroying the sample. The method comprises taking near-infrared spectra of the sample and comparing it visually or mathematically to the spectra of a standard, which may be the values of the spectra known to the operator or may be a standard sample that is provided and run side-by-side.
Inventors
- 👤 Leonard J. Galante
- 👤 Robert A. Lodder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 20:38
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/47; G01N21/49; G01N21/51; C; C12; C12M; C12M41/00; C12M41/30; C12M41/36; G01N2021/4704; G01N2021/4709; G01N21/4738; G01N21/474; G01N2021/4742; G01N2201/00; G01N2201/06; G01N2201/065
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
76 words
A method of detection of microorganisms in a liquid sample to be tested, which comprises i) obtaining near infrared spectra in the range of about 1100 to 1360 nanometers of said liquid sample at a plurality of locations within said liquid sample, ii) obtaining a near infrared spectrum in the range of about 1100 to 1360 nanometers of a standard sample and iii) comparing said liquid sample spectra to said standard sample spectrum with computer assistance.
Claim 2
Independent Claim
76 words
A method of detection of microorganisms in a liquid sample to be tested, which comprises i) obtaining near infrared spectra in the range of about 1100 to 1360 nanometers of said liquid sample at a plurality of locations within said liquid sample, ii) obtaining a near infrared spectrum in the range of about 1100 to 1360 nanometers of a standard sample and iii) comparing said liquid sample spectra to said standard sample spectrum with computer assistance.
Claim 3
Dependent Claim
14 words
The method of claim 1, wherein said liquid sample is an aqueous liquid sample.
Claim 4
Dependent Claim
13 words
The method of claim 1, wherein said microorganisms are yeast bacteria or mold.
Claim 5
Dependent Claim
28 words
The method of claim 1, wherein said liquid sample is held in a container, which is at least partially transparent to at least one wavelength of near-infrared light.
Claim 6
Dependent Claim
16 words
The method of claim 4, wherein said container is a container of glass or a polymer.
Claim 7
Dependent Claim
11 words
The method of claim 5, wherein said polymer is polyvinyl chloride.
Claim 8
Dependent Claim
16 words
The method of claim 1, wherein said liquid sample comprises water and dextrose or sodium chloride.
Claim 9
Dependent Claim
24 words
The method of claim 1, wherein said liquid sample comprises a 5% (w/v) aqueous dextrose solution or a 0.9% (w/v) aqueous sodium chloride solution.
Claim 10
Dependent Claim
38 words
The method of claim 1, wherein said comparison of the near-infrared spectrum of said liquid sample to that of the standard sample is a comparison of the near-infrared scattering spectra of said liquid sample and said standard sample.
Claim 11
Dependent Claim
29 words
The method of claim 1, wherein said method is a method for determining the sterility of an aseptically-filled container for parenteral administration of its liquid contents to a human.
Claim 12
Dependent Claim
12 words
The method of claim 10, wherein said parenteral administration is intravenous administration.
Claim 13
Dependent Claim
16 words
The method of claim 1, wherein said standard sample has a known quantity of said microorganisms.
Claim 14
Dependent Claim
33 words
The method of claim 1, wherein said comparison is carried out by comparing the spectral distribution quantiles of the liquid sample to be tested with the standard as to scale location and shape.
Claim 15
Dependent Claim
21 words
The method of claim 1 wherein said near infrared spectra are obtained at 10 or more locations within said liquid sample.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-02-16 | 1996-08-17 | 1997-02-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-02-16 | 2000-08-17 | 2001-02-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-02-16 | 2004-08-17 | 2005-02-16 | $7700.00 | Window Closed / Lapsed |