US5217876A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for detecting microorganisms
Assignee: Akzo NV
•
Filing: 1992-04-07
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Granted: 1993-06-08
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Expires: 2012-04-07
Abstract
Abstract An instrument and a sealable, sterilizable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium and a sensor means with an indicator medium therein. Changes in the indicator medium resulting from pH change or change in CO 2 concentration in the medium are detected from outside the vessel.
Inventors
- 👤 James E. Turner
- 👤 Thurman C. Thorpe
- 👤 James L. DiGuiseppi
- 👤 Richard C. Driscoll
- 👤 Michael J. Calandra
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 14:08
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/02; C12M23/08; C12M23/38; C12M29/00; C12M29/20; C12M41/00; C12M41/26; C12M41/30; C12M41/36; Y; Y10; Y10S; Y10S435/00; Y10S435/808
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
142 words
A method for detecting microorganisms in a specimen, comprising the steps of introducing the specimen under sterile conditions into a sterile specimen container, the specimen container having at least one transparent section in the wall thereof and a sensor means containing an indicator medium that exhibits a detectable change when exposed to gaseous products of an organism's metabolic activity located inside said container in the region of the transparent section, incubating the specimen in the specimen container with a microbial growth medium, and monitoring from outside the container through the transparent section thereof the production of gaseous products of an organism's metabolic activity without violating the integrity of the container by detecting changes exhibited in the indicator medium in the sensor means resulting from the gaseous products passing to the sensor means, thereby detecting the presence of microorganisms contained in the specimen.
Claim 2
Independent Claim
142 words
A method for detecting microorganisms in a specimen, comprising the steps of introducing the specimen under sterile conditions into a sterile specimen container, the specimen container having at least one transparent section in the wall thereof and a sensor means containing an indicator medium that exhibits a detectable change when exposed to gaseous products of an organism's metabolic activity located inside said container in the region of the transparent section, incubating the specimen in the specimen container with a microbial growth medium, and monitoring from outside the container through the transparent section thereof the production of gaseous products of an organism's metabolic activity without violating the integrity of the container by detecting changes exhibited in the indicator medium in the sensor means resulting from the gaseous products passing to the sensor means, thereby detecting the presence of microorganisms contained in the specimen.
Claim 3
Dependent Claim
19 words
The method of claim 1, wherein the changes exhibited in the indicator medium are monitored using a detector instrument.
Claim 4
Dependent Claim
17 words
The method of claim 1, wherein the specimen is in contact with the sensor means during incubation.
Claim 5
Dependent Claim
32 words
The method of claim 3, wherein the specimen and the microbial growth medium form a liquid composition, and the sensor means is submerged below the surface of that liquid composition during incubation.
Claim 6
Independent Claim
167 words
A method for detecting microorganisms in a specimen, comprising the steps of introducing the specimen under sterile conditions into a sterile specimen container, the specimen container having at least one transparent section in the wall thereof and a sensor means containing an indicator medium that exhibits a detectable change when exposed to gaseous products of an organism's metabolic activity located inside said container in the region of the transparent section, incubating the specimen in the specimen container with a microbial growth medium, and monitoring from outside the container through the transparent section thereof the production of gaseous products of an organism's metabolic activity without violating the integrity of the container by detecting changes exhibited in the indicator medium in the sensor means resulting from the gaseous products passing to the sensor means, wherein the indicator medium in the sensor means is separated from the specimen by a semipermeable membrane, the gaseous products detected comprise CO 2 and the indicator medium is a molecular species responsive to pH.
Claim 7
Independent Claim
167 words
A method for detecting microorganisms in a specimen, comprising the steps of introducing the specimen under sterile conditions into a sterile specimen container, the specimen container having at least one transparent section in the wall thereof and a sensor means containing an indicator medium that exhibits a detectable change when exposed to gaseous products of an organism's metabolic activity located inside said container in the region of the transparent section, incubating the specimen in the specimen container with a microbial growth medium, and monitoring from outside the container through the transparent section thereof the production of gaseous products of an organism's metabolic activity without violating the integrity of the container by detecting changes exhibited in the indicator medium in the sensor means resulting from the gaseous products passing to the sensor means, wherein the indicator medium in the sensor means is separated from the specimen by a semipermeable membrane, the gaseous products detected comprise CO 2 and the indicator medium is a molecular species responsive to pH.
Claim 8
Dependent Claim
19 words
The method of claim 5, wherein the changes exhibited in the indicator medium are monitored using a detector instrument.
Claim 9
Dependent Claim
29 words
The method of claim 5, wherein the specimen and the microbial growth medium form a liquid composition and that liquid composition is in contact with semipermeable membrane during incubation.
Claim 10
Dependent Claim
19 words
The method of claim 7, wherein the membrane is submerged below the surface of the liquid composition during incubation.
Claim 11
Independent Claim
147 words
A method for detecting microorganisms in a specimen, comprising the steps of introducing the specimen under sterile conditions into a sterile specimen container containing a microbial growth medium, the specimen container having at least one transparent section in the wall thereof and a sensor means containing an indicator medium that exhibits a detectable change when exposed to gaseous products of an organism's metabolic activity located inside said container in the region of the transparent section, incubating the specimen in the specimen container, wherein the specimen and microbial growth medium are in contact with the sensor means during incubation, monitoring from outside the container through the transparent section thereof the production of gaseous products of an organism's metabolic activity without violating the integrity of the container by detecting changes exhibited in the indicator medium in the sensor means resulting from the gaseous products passing to the sensor means.
Claim 12
Independent Claim
147 words
A method for detecting microorganisms in a specimen, comprising the steps of introducing the specimen under sterile conditions into a sterile specimen container containing a microbial growth medium, the specimen container having at least one transparent section in the wall thereof and a sensor means containing an indicator medium that exhibits a detectable change when exposed to gaseous products of an organism's metabolic activity located inside said container in the region of the transparent section, incubating the specimen in the specimen container, wherein the specimen and microbial growth medium are in contact with the sensor means during incubation, monitoring from outside the container through the transparent section thereof the production of gaseous products of an organism's metabolic activity without violating the integrity of the container by detecting changes exhibited in the indicator medium in the sensor means resulting from the gaseous products passing to the sensor means.
References Cited (Backward Citations)
US2880070A
Cited
US3067015A
Cited
US3313712A
Cited
US3676679A
Cited
US3853712A
Cited
DE2326028A1
Cited
US4101383A
Cited
USRE31879E
Cited
US4004981A
Cited
US3998591A
Cited
US4003709A
Cited
US4073691A
Cited
US4182656A
Cited
US4201548A
Cited
US4152213A
Cited
US4306877A
Cited
US4236211A
Cited
US4285697A
Cited
WO1981000304A1
Cited
US4330622A
Cited
US4289248A
Cited
US4784947A
Cited
US4456380A
Cited
US4407959A
Cited
JPS57207861A
Cited
US4476870A
Cited
US5155019A
Cited
EP0104463A1
Cited
CA1204606A
Cited
US4557900A
Cited
US4568518A
Cited
US5047331A
Cited
EP0117274A1
Cited
US4672039A
Cited
US4971900A
Cited
US4513280A
Cited
JPS61149818A
Cited
US4698308A
Cited
EP0255087A2
Cited
FR2603685A1
Cited
US4824789A
Cited
US4824789B1
Cited
US4833091A
Cited
EP0301699A2
Cited
US4780191A
Cited
US4889992A
Cited
US5094955A
Cited
EP0333253A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1996-06-07 | 1996-12-07 | 1997-06-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-06-07 | 2000-12-07 | 2001-06-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-06-07 | 2004-12-07 | 2005-06-08 | $7700.00 | Window Closed / Lapsed |