US5272087A
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Enzymatic electrode and its preparation method
Assignee: Centre National de la Recherche Scientifique
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Filing: 1989-04-19
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Granted: 1993-12-21
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Expires: 2009-04-19
Abstract
Abstract PCT No. PCT/FR89/00182 Sec. 371 Date Oct. 16, 1990 Sec. 102(e) Date Oct. 16, 1990 PCT Filed Apr. 19, 1989 PCT Pub. No. WO89/10395 PCT Pub. Date Nov. 2, 1989.An enzymatic electrode, having a homogeneous composition throughout, consists essentially of a uniform intimate admixture of a conducting powder with at least one immobilized enzyme, or with an immobilized enzyme and a mediator agent or a co-enzyme, in a matrix. The electrode is one which has a surface available for direct contact with a substrate without a permeable or semi-permeable intermediate membrane or membranes. The enzymatic electrode is prepared, e.g., by intimately admixing (a) a homogeneous paste or matrix (resulting from an intimate admixture of a conducting powder with a binder), (b) at least one enzyme and (c) an enzyme crosslinking solution, and then incorporating therein a mediator agent and/or a co-enzyme.
Inventors
- 👤 Nabil El Murr
- 👤 Mohamed Slilam
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 04:53
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
62 words
An enzymatic electrode in the form of a matrix comprising a substantially homogenous admixture of a conducting powder and a member selected from the group consisting of (a) at least one immobilized enzyme, (b) an immobilized enzyme and a mediator agent and (c) an immobilized enzyme and a co-enzyme, wherein neither the mediator agent nor the co-enzyme is crosslinked to the matrix.
Claim 2
Dependent Claim
62 words
An enzymatic electrode in the form of a matrix comprising a substantially homogenous admixture of a conducting powder and a member selected from the group consisting of (a) at least one immobilized enzyme, (b) an immobilized enzyme and a mediator agent and (c) an immobilized enzyme and a co-enzyme, wherein neither the mediator agent nor the co-enzyme is crosslinked to the matrix.
Claim 3
Dependent Claim
36 words
An enzymatic electrode of claim 1 having an intimately combined enzymatic part and detection part said electrode having a surface available for direct contact with a substrate without a permeable or semi-permeable intermediate membrane or membranes.
Claim 4
Dependent Claim
17 words
Electrode according to claim 2, which is constructed and adaptable is of the disposable, once-only use, type.
Claim 5
Dependent Claim
16 words
An electrode according to claim 2, wherein the conducting powder is carbon powder or graphite powder.
Claim 6
Dependent Claim
21 words
An electrode according to claim 2, wherein the conducting powder is capable of forming a matrix and physically, confining the enzyme.
Claim 7
Dependent Claim
14 words
An electrode according to claim 2, which contains several enzymes immobilized in the matrix.
Claim 8
Dependent Claim
34 words
An electrode according to claim 2, wherein the matrix is contained in an inert sheath, from which it protrudes at one end to form the surface, which is the useful surface of the electrode.
Claim 9
Dependent Claim
36 words
An electrode according to claim 2, for the determination of glucose, wherein the immobilized enzyme is glucose oxidase and the conducting matrix also contains a mediator selected from the group consisting of p-ferrocenylaniline, nickelocene and benzoquinone.
Claim 10
Dependent Claim
29 words
An electrode according to claim 2, for the determination of sucrose, wherein the at least one immobilized enzyme is invertase and glucose oxidase, or invertase, glucose oxidase and mutarotase.
Claim 11
Dependent Claim
24 words
An electrode according to claim 2, for the determination of ethanol, wherein the immobilized enzyme alcohol dehydrogenase, which is combined with NAD + .
Claim 12
Dependent Claim
40 words
An electrode according to claim 1 constructed and adapted to have a defined surface area for contacting the substrate, the surface area being one which is removable friction or cutting means to expose another surface having the identical surface area.
Claim 13
Dependent Claim
29 words
An electrode according to claim 1 wherein the mediator agent is a member selected from the group consisting of ferrocene, a ferrocene derivative, nickelocene, a nickelocene derivative and benzoquinone.
Claim 14
Dependent Claim
25 words
An electrode according to claim 2, wherein the enzyme is fixed within the matrix of conducting material by means of at least one crosslinking agent.
Claim 15
Dependent Claim
12 words
An electrode according to claim 13, wherein the crosslinking agent is glutaraldehyde.
Claim 16
Dependent Claim
39 words
An electrode according to claim 1 wherein the matrix comprises a conducting paste which is an intimate mixture of the powder with from 10 to 40% by weight of inert binder relative to the weight of the conducting paste.
Claim 17
Dependent Claim
13 words
An electrode according to claim 15, wherein the binder is a hydrophobic binder.
Claim 18
Dependent Claim
18 words
An electrode according to claim 16, wherein the binder consists of paraffin oil, alpha-bromo-naphthalene or a silicone oil.
Claim 19
Dependent Claim
54 words
A process for the preparation of an enzymatic electrode as defined in claim 2 which comprises intimately mixing a homogeneous paste or matrix resulting from the intimate admixture of a conducting powder with a binder; at least one enzyme; and an enzyme crosslinking solution; and then incorporating a mediator agent and/or a co-enzyme therein.
Claim 20
Dependent Claim
51 words
A process according to claim 18, wherein a conducting powder capable of forming a matrix and at least one enzyme are intimately mixed with a mediator agent and/or a co-enzyme and the resulting mixture is subjected to mechanical forces, in order to obtain a solid matrix physically confirming the immobilized enzyme.
Claim 21
Dependent Claim
13 words
A process according to claim 19, wherein the mechanical forces are compression forces.
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