Ruthenium-lumazine energy transfer systems

Claim 2

An energy-transfer system consisting of two organic compounds, one of which is a chromophore of a lumazine type and the other, with which it interacts, is a ruthenium (Ru) complex.

Claim 3

The energy-transfer system of claim 1, wherein the chromophore of the lumazine type is a lumazine derivative of the formula ##STR7## in which R 1 and R 2 each represent an H atom, an optionally substituted C 1-10 -alkyl group, 1'-ribosyl, 1'-(2'-deoxyribosyl) or the radical of an analogous hydroxyl compound; R 3 represents an H atom or represents an optionally substituted C 1-10 -alkyl group; and R 4 represents an optionally substituted C 1-10 -alkyl group; 1'-ribosyl, 1'-(2'-deoxyribosyl) or the radical of an analogous hydroxyl compound, or of the formula ##STR8## in which R 5 and R 6 each represent an optionally substituted C 1-10 -alkyl group; and R 7 and R 8 represent 1'-ribosyl, 1'-(2'-deoxyribosyl) or the radical of an analogous hydroxyl compound.

Claim 4

The energy-transfer system of claim 1 wherein the ruthenium complex is a compound of the formula Ru.sup.2+ L.sub.1 L.sub.2 L.sub.3 III where the ligands L 1 , L 2 and L 3 are identical or different and represent charge-transfer units, and the ligand L 3 is substituted by a group A-X where A is an optionally substituted alkylene group and X is a functional group which does not adversely affect the energy transfer ability of the complex.

Claim 5

The energy-transfer system of claim 3 wherein A represents an alkylene group substituted with sulphonamide, thioether, ether, carboxyl or carboxamide functionalities, and X represents an aldehyde, carboxyl, hydroxyl, amino or thiocyanate group, halogen or a phosphite or phosphate group or a modified phosphate group.

Claim 6

The energy-transfer system of claim 2, wherein the chromophore of the lumazine type is a lumazine derivative of formula I in which R 1 and R 2 each represent an H atom, an optionally substituted C 1-10 -alkyl group, 1'-ribosyl, 1'-(2'-deoxyribosyl) or the radical of an analogous hydroxyl compound; R 3 represents an H atom or represents an optionally substituted C 1-10 -alkyl group; and R 4 represents an optionally substituted C 1-10 -alkyl group, 1'-ribosyl, 1'-(2'-deoxyribosyl) or the radical of an analogous hydroxyl compound.

Claim 7

The energy-transfer system of claim 2, wherein the chromophore of the lumazine type is a lumazine derivative of the formula II in which R 5 and R 6 represent an optionally substituted C 1-10 -alkyl group; and R 7 and R 8 represent 1'-ribosyl, 1'-(2'-deoxyribosyl) or the radical of an analogous hydroxyl compound.

Claim 8

The energy-transfer system according to any of claims 1-6, characterised in that one or more chromophores of the lumazine type are incorporated at the end of a DNA or RNA sequence or within a DNA or RNA sequence and the ruthenium complex is of the formula III in which the charge-transfer units L 1 , L 2 and L 3 are identical or different and represent optionally substituted bipyridyl, bathophenanthroline or benzobathophenanthroline groups, said ruthenium complex being bound to the same or different DNA or RNA sequence as the lumazine.

Claim 9

Energy-transfer system of claim 7 of the following formulae Ru 2+ L 1 L 2 L 3 - Lu-SEQ ID No: 1, Ru 2+ L 1 L 2 L 3 - GTLU-SEQ ID No: 2, Ru 2+ L 1 L 2 L 3 - GTTGALU-SEQ ID No. 3, Ru 2+ L 1 L 2 L 3 - GTTGACAALU-SEQ ID No: 4 or Ru 2+ L 1 L 2 L 3 - LuLuLuLuLu-SEQ ID No: 1 in which the ruthenium complex of the general formula III is linked via a stable phosphodiester linkage to the DNA or RNA, and the chromophores of the lumazine type are 1-(2'-deoxy-α-D-ribofuranosyl)-6,7-dimethyl-lumazine [Lu].