US5288846A
Expired / Lapsed
US
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Cell specific gene regulators
Assignee: General Hospital Corp
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Filing: 1992-08-03
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Granted: 1994-02-22
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Expires: 2012-08-03
Abstract
Abstract Methods and compositions for cell-specific expression of genes are provided. The invention utilizes the endothelin-1 promoter and promoter elements to direct expression of heterologous genes in endothelial cells. The promoters and elements are useful for directing cell-specific expression of therapies for vascular and cardiovascular diseases.
Inventors
- 👤 Thomas Quertermous
- 👤 Mu-En Lee
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 21:13
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N2830/00; C12N2830/008
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
34 words
An isolated DNA molecule comprising an element of the endothelin-1 (ET-1) promoter (SEQ ID No:1) capable of directing high-level cell-specific expression of a heterologous gene in endothelial cells operably linked to a heterologous gene.
Claim 2
Dependent Claim
34 words
An isolated DNA molecule comprising an element of the endothelin-1 (ET-1) promoter (SEQ ID No:1) capable of directing high-level cell-specific expression of a heterologous gene in endothelial cells operably linked to a heterologous gene.
Claim 3
Dependent Claim
20 words
The DNA molecule of claim 1 wherein said DNA comprises at least nucleotides 108 to 250 of SEQ ID NO:1.
Claim 4
Dependent Claim
25 words
The DNA of claim 1 wherein said element is selected from Region A [SEQ ID NO: 2] and Region B [SEQ ID NO: 3] elements.
Claim 5
Dependent Claim
15 words
The DNA of claim 1 wherein said element is Region A [SEQ ID NO: 2].
Claim 6
Dependent Claim
15 words
The DNA of claim 1 wherein said element is Region B [SEQ ID NO: 3].
Claim 7
Dependent Claim
10 words
A cloning vector comprising the DNA molecule of claim 1.
Claim 8
Dependent Claim
11 words
An endothelial cell transformed with the cloning vector of claim 6.
Claim 9
Independent Claim
49 words
A method for directing expression of a gene of interest in vascular endothelial cells, said method comprising: forming a DNA construct wherein a DNA molecule comprising an element of the endothelin-1 (ET-1) promoter[SEQ ID NO:1] is operably linked to said gene; and transforming endothelial cells with said DNA construct.
Claim 10
Independent Claim
49 words
A method for directing expression of a gene of interest in vascular endothelial cells, said method comprising: forming a DNA construct wherein a DNA molecule comprising an element of the endothelin-1 (ET-1) promoter[SEQ ID NO:1] is operably linked to said gene; and transforming endothelial cells with said DNA construct.
Claim 11
Dependent Claim
23 words
The method of claim 8, wherein said DNA sequence comprises Region A [SEQ ID NO:2] and Region B [SEQ ID NO:3] DNA elements.
Claim 12
Dependent Claim
25 words
The method of claim 8 wherein said DNA sequence comprises nucleotides from about -204 to about -86 [SEQ ID NO:4] of the ET-1 promoter sequence.
Claim 13
Dependent Claim
25 words
The method of claim 8 wherein said gene of interest encodes a protein which is a useful for treating thrombosis, thrombolysis, inflammation and immunity disorders.
Claim 14
Dependent Claim
23 words
The method of claim 9 wherein said ET-1 promoter element comprises Region A [SEQ ID NO:2] and Region B [SEQ ID NO:3] DNA.
Claim 15
Dependent Claim
21 words
The DNA molecule of claim 1 wherein said DNA sequence comprises at least nucleotides 47 to 165 of SEQ ID NO:1.
Claim 16
Dependent Claim
21 words
The method of claim 8 wherein said DNA sequence comprises nucleotides from about 108 to about 250 of SEQ ID NO:1.
Claim 17
Dependent Claim
17 words
The method of claim 8, wherein said endothelin-(ET-1) promoter element is operably linked to a heterologous promoter.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-02-21 | 1997-08-23 | 1998-02-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-02-21 | 2001-08-23 | 2002-02-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-02-21 | 2005-08-23 | 2006-02-22 | $7700.00 | Window Closed / Lapsed |