US5310650A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and device for improved reaction kinetics in nucleic acid hybridizations
Assignee: Abbott Laboratories
•
Filing: 1992-08-04
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Granted: 1994-05-10
•
Expires: 2012-08-04
Abstract
Abstract The present invention relates to methods and test strips for performing nucleic acid hybridizations on a porous chromatographic material having the capacity for rapid chromatographic solvent transport of non-immobilized reagents and reactive solution components by means of a selected chromatographic solvent. The strip includes a first end or contact site, at which chromatographic solvent transport begins, a second end, at which chromatographic solvent transport ends, and at least one zone positioned between the first and second ends. The zone is impregnated with a capture nucleotide sequence which is immobilized against solvent transport and which is capable of hybridization with a complementary nucleotide sequence or probe so as to render it immobilized in the capture zone. The strip may further include a probe zone between the first end and the capture zone, wherein a labeled probe is impregnated such that it is mobilized by the solvent and transported to the capture zone where hybridization can occur. Interfering sample components and unhybridized nucleic acids are cleared from the capture zone by chromatographic solvent transport to form an inherent wash.
Inventors
- 👤 Michael E. McMahon
- 👤 Julian Gordon
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 22:25
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5023; C12Q1/6876; C12Q1/6888; C12Q1/689; C12Q1/70; C12Q1/701; B01L2300/00; B01L2300/08; B01L2300/0809; B01L2300/0825; B01L2300/0861; B01L2300/087; B01L2400/00; B01L2400/04; B01L2400/0403; B01L2400/0406; G; G01; G01N; G01N30/00; G01N30/02; G01N30/26; G01N30/38; G01N30/60; G01N30/6052; G01N30/6065; G01N30/90; G01N30/92; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 39
Claim 1
Independent Claim
197 words
A device for performing solid phase hydridizations of soluble, single-stranded polynucleotides, comprising a strip of porous chromatographic medium having: a contact site at or near one end; a capture site remote from the contact side whereat a single-stranded target polynucleotide complementary to a labelled, soluble polynucleotide is immobilized against transport or becomes immobilized against transport by binding to a capture polynucleotide which itself is immobilized against transport; said labelled polynucleotide dried on the medium at a location between the contact site and the remote capture site, such that it can be mobilized and transported to the remote capture site by a transport solution; and capillary means for transporting a solution containing the labelled, soluble polynucleotide under hydridizing conditions to the remote capture site, whereby hybridization can occur between complementary base pairs of the polynucleotides; wherein said strip further comprises a first region, said first region being substantially devoid of capture polynucleotide, adjacent said capture site in the direction of said contact site and a second region adjacent said capture site in the opposite direction, and wherein said strip is nonsegmented between said first region and said capture site and between said second region and said capture site.
Claim 2
Independent Claim
197 words
A device for performing solid phase hydridizations of soluble, single-stranded polynucleotides, comprising a strip of porous chromatographic medium having: a contact site at or near one end; a capture site remote from the contact side whereat a single-stranded target polynucleotide complementary to a labelled, soluble polynucleotide is immobilized against transport or becomes immobilized against transport by binding to a capture polynucleotide which itself is immobilized against transport; said labelled polynucleotide dried on the medium at a location between the contact site and the remote capture site, such that it can be mobilized and transported to the remote capture site by a transport solution; and capillary means for transporting a solution containing the labelled, soluble polynucleotide under hydridizing conditions to the remote capture site, whereby hybridization can occur between complementary base pairs of the polynucleotides; wherein said strip further comprises a first region, said first region being substantially devoid of capture polynucleotide, adjacent said capture site in the direction of said contact site and a second region adjacent said capture site in the opposite direction, and wherein said strip is nonsegmented between said first region and said capture site and between said second region and said capture site.
Claim 3
Dependent Claim
28 words
A device according to claim 1, wherein the porous medium comprises a strip of nitrocellulose having a pore size of between about 0.2 micron and about 10 microns.
Claim 4
Dependent Claim
17 words
A device according to claim 2 wherein the nitrocellulose has a pore size of about 5.0 microns.
Claim 5
Dependent Claim
28 words
A device according to claim 1 wherein said capillary means continues beyond the second region to remove unhybridized polynucleotides and other non-reactive solution components from the remote site.
Claim 6
Dependent Claim
36 words
A device according to claim 1 wherein said capillary means transports said solution at a rate such that a solution front containing said labelled polynucleotide contacts the remote capture site for less than about 10 minutes.
Claim 7
Dependent Claim
31 words
A device according to claim 1 wherein said capillary means transports said solution at a rate such that said solution front contacts the remote site for less than about 2 minutes.
Claim 8
Independent Claim
245 words
A method of detecting the presence of a target polynucleotide comprising: a) providing a porous medium capable of transporting by capillarity a solution of single stranded polynucleotides, said medium having a contact site at or near one end and a capture site remote from the contact site, wherein said strip further comprises a first region, said first region being substantially devoid of capture polynucleotide, adjacent said capture site in the direction of said contact site and a second region adjacent said capture site in the opposite direction, and wherein said strip is nonsegmented between said first region and said capture site and between said second region and said capture site; b) immobilizing a sample suspected to contain target polynucleotide in single-stranded form at said capture site; c) under hybridizing conditions, contacting the contact site with either: i) a transport solvent containing a labelled single-stranded polynucleotide complementary to said target polynucleotide; or ii) a transport solvent capable of mobilizing a labelled single-stranded polynucleotide complementary to said target polynucleotide, said labelled polynucleotide having been previously dried onto a probe site located intermediate the contact site and the remote capture site such that it can be mobilized by transport solvent, the labeled polynucleotide being transported by capillarity to the remote capture site; d) prior to or during step c), blocking nonspecific binding sites on said porous medium; and e) detecting the presence of label, if any, at said remote capture site as a measure of the target polynucleotide.
Claim 9
Independent Claim
245 words
A method of detecting the presence of a target polynucleotide comprising: a) providing a porous medium capable of transporting by capillarity a solution of single stranded polynucleotides, said medium having a contact site at or near one end and a capture site remote from the contact site, wherein said strip further comprises a first region, said first region being substantially devoid of capture polynucleotide, adjacent said capture site in the direction of said contact site and a second region adjacent said capture site in the opposite direction, and wherein said strip is nonsegmented between said first region and said capture site and between said second region and said capture site; b) immobilizing a sample suspected to contain target polynucleotide in single-stranded form at said capture site; c) under hybridizing conditions, contacting the contact site with either: i) a transport solvent containing a labelled single-stranded polynucleotide complementary to said target polynucleotide; or ii) a transport solvent capable of mobilizing a labelled single-stranded polynucleotide complementary to said target polynucleotide, said labelled polynucleotide having been previously dried onto a probe site located intermediate the contact site and the remote capture site such that it can be mobilized by transport solvent, the labeled polynucleotide being transported by capillarity to the remote capture site; d) prior to or during step c), blocking nonspecific binding sites on said porous medium; and e) detecting the presence of label, if any, at said remote capture site as a measure of the target polynucleotide.
Claim 10
Dependent Claim
26 words
The method according to claim 7, wherein the contact site is contacted with a transport solvent containing the labelled single-stranded polynucleotide complementary to said target polynucleotide.
Claim 11
Dependent Claim
75 words
The method according to claim 7, including an additional step, prior to step c, of drying a labelled single-stranded complementary polynucleotide onto the medium at a location between the contact site and the remote site, such that the complementary polynucleotide can be mobilized and transported by the transport solvent, and contacting the contact site with a transport solvent capable of mobilizing the dried single-stranded complementary polynucleotide as recited in step c) ii) of claim 7.
Claim 12
Dependent Claim
36 words
A method according to claim 7 wherein the solution containing the labelled polynucleotide is transported through the medium at a rate such that the labelled polynucleotide contacts the remote site for less than about 5 minutes.
Claim 13
Dependent Claim
37 words
A method according to claim 9 wherein the solution containing the complementary polynucleotide is transported through the medium at a rate such that said solution front contacts the remote capture site for less than about 2 minutes.
Claim 14
Dependent Claim
29 words
A method according to claim 7 wherein transport of solution continues past the second region to remove unhybridized polynucleotides and other non-reactive solution components from the remote capture site.
Claim 15
Dependent Claim
28 words
A method according to claim 7 wherein the porous medium comprises a strip of nitrocellulose having a uniform pore size between about 0.1 micron and about 10 micron.
Claim 16
Dependent Claim
26 words
A method according to claim 7 wherein step d) is performed by pretreating the medium with a blocking agent before contacting it with the transport solvent.
Claim 17
Dependent Claim
20 words
A method according to claim 7 wherein step d) is performed by incorporating a blocking agent into the transport solvent.
Claim 18
Independent Claim
319 words
A method of detecting the presence of a target polynucleotide comprising: a) providing a porous medium capable of transporting by capillarity a solution of single stranded polynucleotides, said medium having a contact site at or near one end, and having immobilized at a capture site remote from said contact site, a single-stranded polynucleotide (capture probe) capable of hybridizing to a first portion of a complementary target polynucleotide, wherein said medium further comprises a first region, said first region being substantially devoid of capture polynucleotide, adjacent said capture site in the direction of said contact site and a second region adjacent said capture site in the opposite direction, and wherein said strip is nonsegmented between said first region and said capture site and between said second region and said capture site; b) causing a sample suspended to contain target polynucleotide to contact said remote capture site, directly or by solvent transport, under hybridizing conditions to permit hybridization of the capture probe with the first portion of any target polynucleotide present; c) during or after step b), contacting the contact site under hybridizing conditions with either: i) a transport solvent containing a labelled single-stranded polynucleotide complementary to a second, distinct portion of said target polynucleotide; or ii) a transport solvent capable of mobilizing a labelled single-stranded polynucleotide complementary to a second distinct portion of said target polynucleotide, said labelled polynucleotide having been previously dried onto a probe site located intermediate the contact site and the remove capture site such that it can be mobilized by transport solvent, the labelled polynucleotide being transported by capillarity to the remote capture site to form a ternary complex of capture probe:target polynucleotide:labelled probe in the presence of target polynucleotide; d) prior to or during step c), blocking nonspecific binding sites on said porous medium; and e) detecting the presence of label, if any, at said remote capture site as a measure of the target polynucleotide.
Claim 19
Independent Claim
319 words
A method of detecting the presence of a target polynucleotide comprising: a) providing a porous medium capable of transporting by capillarity a solution of single stranded polynucleotides, said medium having a contact site at or near one end, and having immobilized at a capture site remote from said contact site, a single-stranded polynucleotide (capture probe) capable of hybridizing to a first portion of a complementary target polynucleotide, wherein said medium further comprises a first region, said first region being substantially devoid of capture polynucleotide, adjacent said capture site in the direction of said contact site and a second region adjacent said capture site in the opposite direction, and wherein said strip is nonsegmented between said first region and said capture site and between said second region and said capture site; b) causing a sample suspended to contain target polynucleotide to contact said remote capture site, directly or by solvent transport, under hybridizing conditions to permit hybridization of the capture probe with the first portion of any target polynucleotide present; c) during or after step b), contacting the contact site under hybridizing conditions with either: i) a transport solvent containing a labelled single-stranded polynucleotide complementary to a second, distinct portion of said target polynucleotide; or ii) a transport solvent capable of mobilizing a labelled single-stranded polynucleotide complementary to a second distinct portion of said target polynucleotide, said labelled polynucleotide having been previously dried onto a probe site located intermediate the contact site and the remove capture site such that it can be mobilized by transport solvent, the labelled polynucleotide being transported by capillarity to the remote capture site to form a ternary complex of capture probe:target polynucleotide:labelled probe in the presence of target polynucleotide; d) prior to or during step c), blocking nonspecific binding sites on said porous medium; and e) detecting the presence of label, if any, at said remote capture site as a measure of the target polynucleotide.
Claim 20
Dependent Claim
26 words
The method according to claim 16, wherein the contact site is contacted with a transport solvent containing the labelled single-stranded polynucleotide complementary to said target polynucleotide.
Claim 21
Dependent Claim
70 words
The method according to claim 16, wherein the contact site is contacted with a transport solvent capable of mobilizing the labelled single-stranded polynucleotide complementary to said target polynucleotide; including an additional step, prior to step d, of drying the single-stranded complementary polynucleotide onto the medium at a location between the contact site and the remote site, such that the complementary polynucleotide can be mobilized and transported by the transport solvent.
Claim 22
Dependent Claim
36 words
A method according to claim 16 wherein the solution containing the labelled polynucleotide is transported through the medium at a rate such that the labelled polynucleotide contacts the remote site for less than about 5 minutes.
Claim 23
Dependent Claim
37 words
A method according to claim 18 wherein the solution containing the complementary polynucleotide is transported through the medium at a rate such that said solution front contacts the remote capture site for less than about 2 minutes.
Claim 24
Dependent Claim
29 words
A method according to claim 16 wherein transport of solution continues past the second region to remove unhybridized polynucleotides and other non-reactive solution components from the remote capture site.
Claim 25
Dependent Claim
28 words
A method according to claim 16 wherein the porous medium comprises a strip of nitrocellulose having a uniform pore size between about 0.1 micron and about 10 microns.
Claim 26
Dependent Claim
26 words
A method according to claim 16 wherein step d) is performed by pretreating the medium with a blocking agent before contacting it with the transport solvent.
Claim 27
Dependent Claim
20 words
A method according to claim 16 wherein step d) is performed by incorporating a blocking agent into the transport solvent.
Claim 28
Independent Claim
260 words
A method of detecting the presence of a target polynucleotide comprising: a) providing a porous medium capable of transporting by capillary a solution of single-stranded polynucleotides, said medium having a contact site at or near one end, and having immobilized at a capture site remote from said contact site, a single-stranded polynucleotide (capture probe) capable of hybridizing to a complementary target polynucleotide, wherein said medium further comprises a first region, said first region being substantially devoid of capture polynucleotide, adjacent said capture site in the direction of said contact site and a second region adjacent said capture site in the opposite direction, and wherein said strip is nonsegmented between said first region and said capture site and between said second region and said capture site; b) labelling any target polynucleotide present in a test sample with a detectable label; c) contacting the contact site under hybridizing conditions with either; i) a transport solvent containing said test sample suspected to contain said labelled target polynucleotide; or ii) a transport solvent capable of mobilizing a test sample suspected to contain said labelled target polynucleotide, said test sample having been previously dried onto a probe site located intermediate the contact site and the remote capture site such that it can be mobilized by transport solvent, the labelled polynucleotide being transported by capillarity to the remote capture site; d) prior to or during step c), blocking nonspecific binding sites on said porous medium; and e) detecting the presence of label, if any, at said remote capture site as a measure of the target polynucleotide.
Claim 29
Independent Claim
260 words
A method of detecting the presence of a target polynucleotide comprising: a) providing a porous medium capable of transporting by capillary a solution of single-stranded polynucleotides, said medium having a contact site at or near one end, and having immobilized at a capture site remote from said contact site, a single-stranded polynucleotide (capture probe) capable of hybridizing to a complementary target polynucleotide, wherein said medium further comprises a first region, said first region being substantially devoid of capture polynucleotide, adjacent said capture site in the direction of said contact site and a second region adjacent said capture site in the opposite direction, and wherein said strip is nonsegmented between said first region and said capture site and between said second region and said capture site; b) labelling any target polynucleotide present in a test sample with a detectable label; c) contacting the contact site under hybridizing conditions with either; i) a transport solvent containing said test sample suspected to contain said labelled target polynucleotide; or ii) a transport solvent capable of mobilizing a test sample suspected to contain said labelled target polynucleotide, said test sample having been previously dried onto a probe site located intermediate the contact site and the remote capture site such that it can be mobilized by transport solvent, the labelled polynucleotide being transported by capillarity to the remote capture site; d) prior to or during step c), blocking nonspecific binding sites on said porous medium; and e) detecting the presence of label, if any, at said remote capture site as a measure of the target polynucleotide.
Claim 30
Dependent Claim
26 words
The method according to claim 25, wherein the contact site is contacted with a transport solvent containing the labelled single-stranded polynucleotide complementary to said target polynucleotide.
Claim 31
Dependent Claim
70 words
The method according to claim 25, wherein the contact site is contacted with a transport solvent capable of mobilizing the labelled single-stranded polynucleotide complementary to said target polynucleotide; including an additional step, prior to step d, of drying the single-stranded complementary polynucleotide onto the medium at a location between the contact site and the remote site, such that the complementary polynucleotide can be mobilized and transported by the transport solvent.
Claim 32
Dependent Claim
36 words
A method according to claim 25 wherein the solution containing the labelled polynucleotide is transported through the medium at a rate such that the labelled polynucleotide contacts the remote site for less than about 5 minutes.
Claim 33
Dependent Claim
37 words
A method according to claim 27 wherein the solution containing the complementary polynucleotide is transported through the medium at a rate such that said solution front contacts the remote capture site for less than about 2 minutes.
Claim 34
Dependent Claim
29 words
A method according to claim 25 wherein transport of solution continues past the second region to remove unhybridized polynucleotides and other non-reactive solution components from the remote capture site.
Claim 35
Dependent Claim
28 words
A method according to claim 25 wherein the porous medium comprises a step or nitrocellulose having a uniform pore size between about 0.1 micron and about 10 microns.
Claim 36
Dependent Claim
26 words
A method according to claim 25 wherein step d) is performed by pretreating the medium with a blocking agent before contacting it with the transport solvent.
Claim 37
Dependent Claim
20 words
A method according to claim 25 wherein step d) is performed by incorporating a blocking agent into the transport solvent.
Claim 38
Dependent Claim
277 words
In a solid phase hybridization method for detecting the presence of a target polynucleotide involving: immobilizing a target polynucleotide, if present in a test sample, directly or via an intermediate capture structure, on a solid phase at a capture site; before, during or after said immobilization, attaching a detectable label to said target polynucleotide, if present; and detecting said label, if any, at said capture site; the improvement comprising: a) using a porous chromatographic medium as said solid phase, said medium having a contact site at one end and said capture site being remote from said contact site, wherein said medium further comprises a first region, said first region being substantially devoid of capture polynucleotide, adjacent said capture site in the direction of said contact site and a second region adjacent said capture site in the opposite direction, and wherein said strip is nonsegmented between said first region and said capture site and between said second region and said capture site, and optionally including at least one other site intermediate said contact site and said capture site; and b) performing at least one of said immobilizing and attaching steps by immobilizing at least one single-stranded polynucleotide in hybridizable form at said capture site and transporting by chromatographic solvent transport from said contact site or said other site at least one single-stranded labelled complementary polynucleotide capable of hybridizing with said immobilized polynucleotide, such that label becomes immobilized at said capture site if target polynucleotide is present; wherein at least one hybridization step occurs between an immobilized polynucleotide on said solid phase and a complementary polynucleotide in a mobile phase transported past said immobilized polynucleotide by chromatographic solvent transport.
Claim 39
Dependent Claim
277 words
In a solid phase hybridization method for detecting the presence of a target polynucleotide involving: immobilizing a target polynucleotide, if present in a test sample, directly or via an intermediate capture structure, on a solid phase at a capture site; before, during or after said immobilization, attaching a detectable label to said target polynucleotide, if present; and detecting said label, if any, at said capture site; the improvement comprising: a) using a porous chromatographic medium as said solid phase, said medium having a contact site at one end and said capture site being remote from said contact site, wherein said medium further comprises a first region, said first region being substantially devoid of capture polynucleotide, adjacent said capture site in the direction of said contact site and a second region adjacent said capture site in the opposite direction, and wherein said strip is nonsegmented between said first region and said capture site and between said second region and said capture site, and optionally including at least one other site intermediate said contact site and said capture site; and b) performing at least one of said immobilizing and attaching steps by immobilizing at least one single-stranded polynucleotide in hybridizable form at said capture site and transporting by chromatographic solvent transport from said contact site or said other site at least one single-stranded labelled complementary polynucleotide capable of hybridizing with said immobilized polynucleotide, such that label becomes immobilized at said capture site if target polynucleotide is present; wherein at least one hybridization step occurs between an immobilized polynucleotide on said solid phase and a complementary polynucleotide in a mobile phase transported past said immobilized polynucleotide by chromatographic solvent transport.
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