US5318890A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Assays for inhibitors of leukocyte adhesion
Assignee: University of California
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Filing: 1991-05-06
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Granted: 1994-06-07
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Expires: 2011-05-06
Abstract
Abstract The present invention provides novel assays for determining the ability of a test compound to inhibit intercellular adhesion mediated by a selectin receptor, such as the LHR. The assays involve contacting the test compound with the receptor and an isolated receptor-binding agent. The receptor-binding agent is a sulfated polysaccharide, a sulfated glycolipid, or a compound comprising the extracellular region of an endothelial cell surface glycoprotein. Also provided are compositions comprising a compound comprising the extracellular region of an endothelial cell surface protein, which is specifically recognized by lymphocyte homing receptors.
Inventors
- 👤 Steven Rosen
- 👤 Mark Singer
- 👤 Yasuyuki Imai
- 👤 Ted Yednock
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 23:21
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4727; A; A61; A61P; A61P29/00; C07K14/705; C07K14/7056; C07K14/70564; C07K14/70596; C07K16/00; C07K16/18; C07K16/28; C07K16/2851; C07K16/2854; A61K; A61K38/00; C07K2317/00; C07K2317/30; C07K2317/34; C07K2319/00; C07K2319/33
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
97 words
A method of assaying a test compound for the ability to inhibit intercellular adhesion mediated by a lymphocyte homing receptor, the method comprising the steps of: contacting the test compound with an isolated receptor-binding agent and a chimeric molecule comprising a lymphocyte homing receptor component and an immunoglobulin component, the agent being selected from the group consisting of a phosphorylated polysaccharide, a sulfated polysaccharide, a sulfated glycolipid, and a compound comprising the extracellular region of an endothelial cell surface glycoprotein; and detecting the ability of the test compound to inhibit binding between the receptor and the agent.
Claim 2
Independent Claim
97 words
A method of assaying a test compound for the ability to inhibit intercellular adhesion mediated by a lymphocyte homing receptor, the method comprising the steps of: contacting the test compound with an isolated receptor-binding agent and a chimeric molecule comprising a lymphocyte homing receptor component and an immunoglobulin component, the agent being selected from the group consisting of a phosphorylated polysaccharide, a sulfated polysaccharide, a sulfated glycolipid, and a compound comprising the extracellular region of an endothelial cell surface glycoprotein; and detecting the ability of the test compound to inhibit binding between the receptor and the agent.
Claim 3
Dependent Claim
14 words
A method of claim 1 wherein the receptor is immobilized on a solid surface.
Claim 4
Dependent Claim
25 words
A method of claim 1 wherein the step of detecting the binding of the receptor-binding agent to the receptor is carried out using flow cytometry.
Claim 5
Dependent Claim
11 words
A method of claim 1 wherein the receptor-binding agent is PPME.
Claim 6
Dependent Claim
14 words
A method of claim 1 wherein the receptor-binding agent is fucoidin or dextran sulfate.
Claim 7
Dependent Claim
12 words
A method of claim 1 wherein the receptor binding agent is sulfatide.
Claim 8
Dependent Claim
15 words
A method of claim 1 wherein the glycoprotein is sgp 50 or Sgp 90 .
Claim 9
Dependent Claim
11 words
A method of claim 1 wherein the receptor-binding agent is labeled.
Claim 10
Dependent Claim
13 words
A method of claim 8 wherein the receptor-binding agent is labeled with fluorescein.
Claim 11
Independent Claim
63 words
A method of assaying a carbohydrate test compound for the ability to inhibit intercellular adhesion mediated by a lymphocyte homing receptor, the method comprising the steps of: contacting the test compound with a fluoresceinated receptor-binding agent and a lymphocyte; and detecting the ability of the test compound to inhibit binding of the receptor-binding agent to the cell using a fluorescence activated flow cytometer.
Claim 12
Independent Claim
63 words
A method of assaying a carbohydrate test compound for the ability to inhibit intercellular adhesion mediated by a lymphocyte homing receptor, the method comprising the steps of: contacting the test compound with a fluoresceinated receptor-binding agent and a lymphocyte; and detecting the ability of the test compound to inhibit binding of the receptor-binding agent to the cell using a fluorescence activated flow cytometer.
Claim 13
Dependent Claim
36 words
A method of claim 10 wherein the fluoresceinated receptor-binding agent is selected from the group consisting of a sulfated polysaccharide, a sulfated glycolipid, and a compound comprising the extracellular region of an endothelial cell surface glycoprotein.
Claim 14
Dependent Claim
12 words
A method of claim 10 wherein the fluoresceinated receptor-binding agent is fucoidin.
Claim 15
Independent Claim
96 words
A method of assaying a test compound for the ability to inhibit intercellular adhesion mediated by a chimeric molecule comprising a lymphocyte homing receptor and an immunoglobulin component, the method comprising the steps of: contacting the test compound with a chimeric molecule comprising a lymphocyte homing receptor component and an immunoglobulin component and an isolated receptor-binding agent comprising the extracellular region of an endothelial cell surface glycoprotein selected from the group consisting of Sgp 50 or Sgp 90 ; and detecting the ability of the test compound to inhibit binding between the receptor and the agent.
Claim 16
Independent Claim
96 words
A method of assaying a test compound for the ability to inhibit intercellular adhesion mediated by a chimeric molecule comprising a lymphocyte homing receptor and an immunoglobulin component, the method comprising the steps of: contacting the test compound with a chimeric molecule comprising a lymphocyte homing receptor component and an immunoglobulin component and an isolated receptor-binding agent comprising the extracellular region of an endothelial cell surface glycoprotein selected from the group consisting of Sgp 50 or Sgp 90 ; and detecting the ability of the test compound to inhibit binding between the receptor and the agent.
Claim 17
Dependent Claim
14 words
A method of claim 13 wherein the receptor is immobilized on a solid surface.
Claim 18
Dependent Claim
11 words
A method of claim 13 wherein the receptor-binding agent is labeled.
Claim 19
Independent Claim
72 words
A method of assaying a test compound for the ability to inhibit intercellular adhesion mediated by a lymphocyte homing receptor, the method comprising the steps of: immobilizing the test compound on a solid surface; contacting the test compound with a chimeric molecule comprising a lymphocyte homing receptor component and an immunoglobulin component; and detecting the ability of the lymphocyte homing receptor component of the chimeric molecule to bind to the test compound.
Claim 20
Independent Claim
72 words
A method of assaying a test compound for the ability to inhibit intercellular adhesion mediated by a lymphocyte homing receptor, the method comprising the steps of: immobilizing the test compound on a solid surface; contacting the test compound with a chimeric molecule comprising a lymphocyte homing receptor component and an immunoglobulin component; and detecting the ability of the lymphocyte homing receptor component of the chimeric molecule to bind to the test compound.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-06-06 | 1997-12-06 | 1998-06-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-06-06 | 2001-12-06 | 2002-06-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-06-06 | 2005-12-06 | 2006-06-07 | $7700.00 | Window Closed / Lapsed |