US5382510A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods of diagnosing pre-cancer or cancer states using probes for detecting mutant p53
Assignee: Princeton University
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Filing: 1992-07-10
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Granted: 1995-01-17
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Expires: 2012-07-10
Abstract
Abstract A panel of probes that detect and distinguish between sets of human p53 gene or protein mutations that frequently occur or are selected for in pre-cancer and cancer cells, each set giving rise to a phenotype that is different from that of wild-type p53 and of at least one other set of p53 mutants.
Inventors
- 👤 Arnold J. Levine
- 👤 Thomas E. Shenk
- 👤 Cathy A. Finlay
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 10:41
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/32; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4746; C07K14/475; C07K16/22; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6883; C12Q1/6886; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/5758; G01N33/57595; C12Q2600/00; C12Q2600/156; G01N2333/00; G01N2333/82
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
133 words
A method of correlating a set of human p53 gene or protein point mutations in pre-cancer or cancer cells with a phenotype, the method comprising: (a) determining which set of human p53 gene or protein mutations point is present in a sample of pre-cancer or cancer cells with a probe or probes that can distinguish between sets of human p53 gene or protein point mutations, each set of point mutations correlating with a phenotype that is different from the phenotype associated with the wild-type p53 gene or protein and from the phenotype associated with at least one other set of point mutations; and (b) correlating the set of gene or protein point mutations with its transformation frequency, half-life, or ability to bind to the heat shock protein hsc70, or a phenotype related thereto.
Claim 2
Independent Claim
133 words
A method of correlating a set of human p53 gene or protein point mutations in pre-cancer or cancer cells with a phenotype, the method comprising: (a) determining which set of human p53 gene or protein mutations point is present in a sample of pre-cancer or cancer cells with a probe or probes that can distinguish between sets of human p53 gene or protein point mutations, each set of point mutations correlating with a phenotype that is different from the phenotype associated with the wild-type p53 gene or protein and from the phenotype associated with at least one other set of point mutations; and (b) correlating the set of gene or protein point mutations with its transformation frequency, half-life, or ability to bind to the heat shock protein hsc70, or a phenotype related thereto.
Claim 3
Dependent Claim
39 words
A method according to claim 1 wherein one set of mutations comprises a mutation at amino acid position 143 or 175 of the p53 protein or at codon 143 or 175 of a DNA molecule encoding the p53 protein.
Claim 4
Dependent Claim
39 words
A method according to claim 1 wherein one set of mutations comprises a mutation at amino acid position 273 or 281 of the p53 protein or at codon 273 or 281 of a DNA molecule encoding the p53 protein.
Claim 5
Dependent Claim
11 words
A method according to claim 1 wherein the probes are antibodies.
Claim 6
Dependent Claim
12 words
A method according to claim 1 wherein the probes are DNA probes.
Claim 7
Dependent Claim
11 words
A method according to claim 4 wherein the antibodies are monoclonal.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-01-16 | 1998-07-18 | 1999-01-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-01-16 | 2002-07-18 | 2003-01-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-01-16 | 2006-07-18 | 2007-01-17 | $7700.00 | Window Closed / Lapsed |