US5384246A
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Determination of ions in fluids by a process involving displacement of indicator ions
Assignee: Flinders University
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Filing: 1992-06-22
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Granted: 1995-01-24
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Expires: 2012-06-22
Abstract
Abstract A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.
Inventors
- 👤 Michael N. Berry
- 👤 Michael-Harold Town
- 👤 Georg-Burkhard Kresse
- 👤 Uwe Herrmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 01:14
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; C12Q1/34; C12Q1/37; C12Q1/40; C12Q1/48; C12Q1/527; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/64; G01N33/84; C12Q2334/00; C12Q2334/10; C12Q2337/00; C12Q2337/10; C12Q2337/12; G01N2333/00; G01N2333/90; G01N2333/91; G01N2333/912; G01N2333/91205; G01N2333/9121; G01N2333/914; G01N2333/924; Y; Y10; Y10S; Y10S435/00; Y10S435/962
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
111 words
Process for the determination of the concentration of an analyte ion in a fluid sample, comprising measuring the activity of an enzyme stimulated by an indicator ion, said enzyme selected from the group consisting of a transferase, a hydrolase, an oxidoreductase or a lyase in the presence of a selective binding agent and said indicator ion, wherein the selective binding agent forms a complex with the indicator ion and the indicator ion is displaced stoichiometrically from the complex by the analyte ion, whereby the displaced indicator ion stimulates the activity of the enzyme, wherein the activity of the enzyme is proportional to the concentration of analyte ion in the fluid sample.
Claim 2
Dependent Claim
111 words
Process for the determination of the concentration of an analyte ion in a fluid sample, comprising measuring the activity of an enzyme stimulated by an indicator ion, said enzyme selected from the group consisting of a transferase, a hydrolase, an oxidoreductase or a lyase in the presence of a selective binding agent and said indicator ion, wherein the selective binding agent forms a complex with the indicator ion and the indicator ion is displaced stoichiometrically from the complex by the analyte ion, whereby the displaced indicator ion stimulates the activity of the enzyme, wherein the activity of the enzyme is proportional to the concentration of analyte ion in the fluid sample.
Claim 3
Dependent Claim
11 words
Process according to claim 1, wherein said fluid sample is water.
Claim 4
Dependent Claim
13 words
Process according to claim 1, wherein said fluid sample is a body fluid.
Claim 5
Dependent Claim
18 words
Process according to claim 3, wherein said body fluid is blood, serum, plasma, urine, sweat, exudates or transudates.
Claim 6
Dependent Claim
14 words
Process according to claim 3, wherein the enzyme is a transferase transferring phosphorus-containing groups.
Claim 7
Dependent Claim
25 words
Process according to claim 5, wherein the transferase is selected from the group consisting of pyruvate kinase, hexokinase, adenylate kinase, pyridoxal kinase or acetate kinase.
Claim 8
Dependent Claim
20 words
Process according to claim 3, wherein the enzyme is selected from the group consisting of a hydrolase or a protease.
Claim 9
Dependent Claim
32 words
Process according to claim 7, wherein the hydrolase is selected from the group consisting of α- or β-D-galactosidase, carboxypeptidase A, collagenase, amylase, Calpain I, Calpain II, or dipeptidyl aminopeptidase I (Cathepsin C).
Claim 10
Dependent Claim
15 words
Process according to claim 8, wherein the hydrolase is α- or β-D-galactosidase or carboxypeptidase A.
Claim 11
Dependent Claim
11 words
Process according to claim 3, wherein the enzyme is an oxidoreductase.
Claim 12
Dependent Claim
21 words
Process according to claim 10, wherein the oxidoreductase is selected from the group consisting of glycerol dehydrogenase, acetaldehyde dehydrogenase or tyrosinase.
Claim 13
Dependent Claim
11 words
Process according to claim 3, wherein the enzyme is a lyase.
Claim 14
Dependent Claim
16 words
Process according to claim 12, wherein the lyase is a carbon-carbon lyase or a carbon-oxygen lyase.
Claim 15
Dependent Claim
13 words
Process according to claim 12, wherein the lyase is aldolase or carbonic anhydrase.
Claim 16
Dependent Claim
38 words
Process according to claim 3, wherein the analyte ion is selected from the group consisting of potassium, sodium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron, chloride, ammonium or substances that give rise to the formation of ammonium.
Claim 17
Dependent Claim
28 words
Process according to claim 3, wherein the analyte ion is potassium and the enzyme is selected from the group consisting of pyruvate kinase, glycerol dehydrogenase or acetaldehyde dehydrogenase.
Claim 18
Dependent Claim
19 words
Process according to claim 3, wherein the analyte ion is sodium and the enzyme is pyruvate kinase or β-D-galactosidase.
Claim 19
Dependent Claim
47 words
Process according to claim 3, wherein the selective binding agent is selected from the group consisting of a cryptand, coronand, podand, crown ether, spherand. hemispherand, calixaren, a natural occurring ionophore, a cyclic peptide, a complexone or a chelating agent, an alkali metal, or an alkaline earth metal.
Claim 20
Dependent Claim
27 words
Process according to claim 3, wherein the enzyme is pyruvate kinase, the indicator ion is potassium, the binding agent Kryptofix 221 and the analyte ion is sodium.
Claim 21
Dependent Claim
31 words
Process according to claim 3, wherein the enzyme is kinase, the indicator ion is Mg 2+ , the binding agent is a chelating agent and the analyte ion is a metal.
Claim 22
Dependent Claim
32 words
Process according to claim 3, wherein the enzyme is pyridoxal kinase, the indicator ion is Zn 2+ , the binding agent is Kryptofix 221 and the analyte ion is a heavy metal.
Claim 23
Dependent Claim
23 words
Process according to claim 3, wherein said selective binding agent is selected from the group consisting of a cryptand and a chelating agent.
Claim 24
Dependent Claim
93 words
A composition for the determination of the concentration of an analyte ion in a fluid sample, comprising an indicator ion, an enzyme stimulated by said indicator ion, said enzyme selected from the group consisting of a transferase, a hydrolase, an oxidoreductase or a lyase and a selective binding agent that forms a complex with the indicator ion whereby the indicator ion is displaced stoichiometrically from the complex by said analyte ion in said fluid sample wherein the activity of the enzyme is proportional to the concentration of analyte ion in the fluid sample.
Claim 25
Dependent Claim
93 words
A composition for the determination of the concentration of an analyte ion in a fluid sample, comprising an indicator ion, an enzyme stimulated by said indicator ion, said enzyme selected from the group consisting of a transferase, a hydrolase, an oxidoreductase or a lyase and a selective binding agent that forms a complex with the indicator ion whereby the indicator ion is displaced stoichiometrically from the complex by said analyte ion in said fluid sample wherein the activity of the enzyme is proportional to the concentration of analyte ion in the fluid sample.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-01-23 | 1998-07-25 | 1999-01-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-01-23 | 2002-07-25 | 2003-01-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-01-23 | 2006-07-25 | 2007-01-24 | $7700.00 | Window Closed / Lapsed |