Bone-related carboxypeptidase-like protein and process for its production

Claim 2

DNA coding for a protein comprising OSF-5 having an amino acid sequence at the 26th to 1128th positions in Sequence ID No. 1 of the Sequence Listing.

Claim 3

DNA coding for a protein comprising an OSF-5 precursor protein having an amino acid sequence at the 1st to 1128th positions, including a signal peptide at the 1st to 25th positions, in Sequence ID No. 1 of the Sequence Listing.

Claim 4

DNA coding for a protein comprising an OSF-5 precursor protein having an amino acid sequence at the 1st to 1128th positions, including a signal peptide at the 1st to 25th positions, in Sequence ID No. 1 of the Sequence Listing.

Claim 5

A process for the production of a recombinant mammalian OSF-5 protein, wherein said recombinant mammalian OSF-5 protein comprises an amino acid sequence at the 26th to 1128th positions in Sequence ID No. 1 of the Sequence Listing, comprising the steps of: (a) obtaining a population of cells containing a DNA composed of the following DNA sequences: (i) a sequence that can function in the cells to control transcription and translation, and (ii) a DNA sequence joined downstream of said controlling sequence to code for said recombinant protein, and (b) culturing said population of cells under conditions permit the production of said recombinant protein.

Claim 6

A process for the production of a recombinant mammalian OSF-5 protein, wherein said recombinant mammalian OSF-5 protein comprises an amino acid sequence at the 26th to 1128th positions in Sequence ID No. 1 of the Sequence Listing, comprising the steps of: (a) obtaining a population of cells containing a DNA composed of the following DNA sequences: (i) a sequence that can function in the cells to control transcription and translation, and (ii) a DNA sequence joined downstream of said controlling sequence to code for said recombinant protein, and (b) culturing said population of cells under conditions permit the production of said recombinant protein.

Claim 7

The process of claim 3 wherein the controlling sequence further contains a DNA coding for signal peptide for secreting said recombinant protein extracellularly such that said DNA is positioned immediately upstream of said DNA sequence coding for said recombinant protein.

Claim 8

The process of claim 3 wherein the population of cells is Escherichia coli, or yeast, or mammalian cells. __________________________________________________________________________ SEQUENCE LISTING (1) GENERAL INFORMATION: (iii) NUMBER OF SEQUENCES: 27 (2) INFORMATION FOR SEQ ID NO:1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3728 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA to mRNA (vi) ORIGINAL SOURCE: (A) ORGANISM: Mus musculus (B) STRAIN: osteoblastic cell line MC3T3E1 (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 69..3452 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1: GAATTCGCGGCCGCCTGCCACCCAAGTCCCTGCTCAAGCCCGCCCGGCTCCCGCGCGTGC60 CCAGAGCCATGGCT CCAGTGCGCACCGCATCCCTGCTCTGCGGCCTCCTG110 MetAlaProValArgThrAlaSerLeuLeuCysGlyLeuLeu 1510 GCACTGCTGACGCTGTG CCCTGAGGGGAACCCACAGACGGTGCTGACG158 AlaLeuLeuThrLeuCysProGluGlyAsnProGlnThrValLeuThr 15202530 GACGACGAGAT CGAGGAGTTCCTCGAAGGCTTCCTTTCGGAGTTGGAG206 AspAspGluIleGluGluPheLeuGluGlyPheLeuSerGluLeuGlu 354045 ACCCAGTC CCCGCCCCGGGAAGACGACGTGGAAGTCCAGCCGCTTCCC254 ThrGlnSerProProArgGluAspAspValGluValGlnProLeuPro 505560 GAACCCAC CCAGCGTCCCCGCAAATCCAAGGCAGGGGGCAAGCAGCGG302 GluProThrGlnArgProArgLysSerLysAlaGlyGlyLysGlnArg 657075 GCAGATGTAGA AGTCCCTCCAGAAAAAAACAAAGACAAAGAGAAGAAA350 AlaAspValGluValProProGluLysAsnLysAspLysGluLysLys 808590 GGAAAGAAGGACAAAGG CCCCAAAGCCACAAAACCCCTGGAGGGCTCT398 GlyLysLysAspLysGlyProLysAlaThrLysProLeuGluGlySer 95100105110 ACCAGGCCCAC CAAGAAACCAAAGGAGAAGCCACCCAAGGCCACCAAG446 ThrArgProThrLysLysProLysGluLysProProLysAlaThrLys 115120125 AAGCCCAA GGAGAAACCACCCAAGGCCACCAAGAAGCCCAAGGAGAAG494 LysProLysGluLysProProLysAlaThrLysLysProLysGluLys 130135140 CCACCCAA GGCCACCAAGAAGCCTAAGGAGAAGCCACCCAAGGCCACT542 ProProLysAlaThrLysLysProLysGluLysProProLysAlaThr 145150155 AAGAGGCCCTC GGCAGGAAAGAAGTTCTCAACTGTGGCCCCCTTGGAA590 LysArgProSerAlaGlyLysLysPheSerThrValAlaProLeuGlu 160165170 ACGCTGGATCGGTTACT CCCCTCACCCTCCAACCCCAGCGCCCAGGAG638 ThrLeuAspArgLeuLeuProSerProSerAsnProSerAlaGlnGlu 175180185190 CTACCGCAGAA GAGAGACACACCCTTCCCAAATGCCTGGCAAGGTCAA686 LeuProGlnLysArgAspThrProPheProAsnAlaTrpGlnGlyGln 195200205 GGAGAAGA GACCCAGGTGGAGGCCAAGCAGCCCCGGCCAGAGCCAGAG734 GlyGluGluThrGlnValGluAlaLysGlnProArgProGluProGlu 210215220 GAGGAGAC TGAGATGCCCACACTGGACTACAATGACCAGATAGAGAAG782 GluGluThrGluMetProThrLeuAspTyrAsnAspGlnIleGluLys 225230235 GAGGATTACGA GGATTTTAAGTACATCCTTTGCCAGAAGCAGCCCAGG830 GluAspTyrGluAspPheLysTyrIleLeuCysGlnLysGlnProArg 240245250 CCAACACCCAGCAGGAG GAGGCTCTGGCCAGAGCGCCCTGAGGAGAAG878 ProThrProSerArgArgArgLeuTrpProGluArgProGluGluLys 255260265270 ACTGAAGAGCC AGAGGAAAGGAAGGAAGTCGAGCCACCTCTGAAGCCC926 ThrGluGluProGluGluArgLysGluValGluProProLeuLysPro 275280285 CTGCTGCC TCCGGACTATGGGGATAGCTACGTGATCCCCAACTATGAT974 LeuLeuProProAspTyrGlyAspSerTyrValIleProAsnTyrAsp 290295300 GACTTGGA CTATTATTTCCCCCACCCTCCACCGCAGAAGCCTGATGTT1022 AspLeuAspTyrTyrPheProHisProProProGlnLysProAspVal 305310315 GGACAAGAGGT GGATGAGGAAAAGGAAGAGATGAAGAAGCCCAAAAAG1070 GlyGlnGluValAspGluGluLysGluGluMetLysLysProLysLys 320325330 GAGGGTAGTAGCCCCAA GGAGGACACAGAGGACAAGTGGACCGTGGAG1118 GluGlySerSerProLysGluAspThrGluAspLysTrpThrValGlu 335340345350 AAAAACAAGGA CCACAAAGGGCCCCGGAAGGGTGAGGAGCTGGAGGAG1166 LysAsnLysAspHisLysGlyProArgLysGlyGluGluLeuGluGlu 355360365 GAGTGGGC GCCAGTGGAGAAAATCAAGTGCCCACCTATTGGGATGGAG1214 GluTrpAlaProValGluLysIleLysCysProProIleGlyMetGlu 370375380 TCACACCG CATTGAGGACAACCAGATCCGTGCCTCCTCCATGCTGCGC1262 SerHisArgIleGluAspAsnGlnIleArgAlaSerSerMetLeuArg 385390395 CACGGCCTCGG AGCCCAGCGGGGCCGGCTCAACATGCAGGCTGGTGCC1310 HisGlyLeuGlyAlaGlnArgGlyArgLeuAsnMetGlnAlaGlyAla 400405410 AATGAAGATGACTACTA TGACGGGGCATGGTGTGCTGAGGACGAGTCG1358 AsnGluAspAspTyrTyrAspGlyAlaTrpCysAlaGluAspGluSer 415420425430 CAGACCCAGTG GATCGAGGTGGACACCCGAAGGACAACTCGGTTCACG1406 GlnThrGlnTrpIleGluValAspThrArgArgThrThrArgPheThr 435440445 GGCGTCAT CACTCAGGGCCGTGACTCCAGCATCCATGACGACTTCGTG1454 GlyValIleThrGlnGlyArgAspSerSerIleHisAspAspPheVal 450455460 ACTACCTT CTTTGTGGGCTTCAGCAATGACAGCCAGACCTGGGTGATG1502 ThrThrPhePheValGlyPheSerAsnAspSerGlnThrTrpValMet 465470475 TACACCAATGG CTACGAGGAAATGACCTTCTATGGAAATGTGGACAAG1550 TyrThrAsnGlyTyrGluGluMetThrPheTyrGlyAsnValAspLys 480485490 GACACACCTGTGCTGAG CGAGCTCCCTGAGCCAGTTGTGGCCCGTTTC1598 AspThrProValLeuSerGluLeuProGluProValValAlaArgPhe 495500505510 ATCCGCATCTA TCCACTCACCTGGAACGGTAGCCTGTGCATGCGCCTG1646 IleArgIleTyrProLeuThrTrpAsnGlySerLeuCysMetArgLeu 515520525 GAGGTGCT AGGCTGCCCCGTGACCCCTGTCTACAGCTACTACGCACAG1694 GluValLeuGlyCysProValThrProValTyrSerTyrTyrAlaGln 530535540 AATGAGGT GGTAACTACTGACAGCCTGGACTTCCGGCACCACAGCTAC1742 AsnGluValValThrThrAspSerLeuAspPheArgHisHisSerTyr 545550555 AAGGACATGCG CCAGCTGATGAAGGCTGTCAATGAGGAGTGCCCCACA1790 LysAspMetArgGlnLeuMetLysAlaValAsnGluGluCysProThr 560565570 ATCACTCGCACATACAG CCTGGGCAAGAGTTCACGAGGGCTCAAGATC1838 IleThrArgThrTyrSerLeuGlyLysSerSerArgGlyLeuLysIle 575580585590 TACGCAATGGA AATCTCAGACAACCCTGGGGATCATGAACTGGGGGAG1886 TyrAlaMetGluIleSerAspAsnProGlyAspHisGluLeuGlyGlu 595600605 CCCGAGTT CCGCTACACAGCCGGGATCCACGGCAATGAGGTGCTAGGC1934 ProGluPheArgTyrThrAlaGlyIleHisGlyAsnGluValLeuGly 610615620 CGAGAGCT CCTGCTCCTGCTCATGCAATACCTATGCCAGGAGTACCGC1982 ArgGluLeuLeuLeuLeuLeuMetGlnTyrLeuCysGlnGluTyrArg 625630635 GATGGGAACCC GAGAGTGCGCAACCTGGTGCAGGACACACGCATCCAC2030 AspGlyAsnProArgValArgAsnLeuValGlnAspThrArgIleHis 640645650 CTGGTGCCCTCGCTGAA CCCTGATGGCTATGAGGTGGCAGCGCAGATG2078 LeuValProSerLeuAsnProAspGlyTyrGluValAlaAlaGlnMet 655660665670 GGCTCAGAGTT TGGGAACTGGGCACTGGGGCTGTGGACTGAGGAGGGC2126 GlySerGluPheGlyAsnTrpAlaLeuGlyLeuTrpThrGluGluGly 675680685 TTTGACAT CTTCGAGGACTTCCCAGATCTCAACTCTGTGCTCTGGGCA2174 PheAspIlePheGluAspPheProAspLeuAsnSerValLeuTrpAla 690695700 GCTGAGGA GAAGAAATGGGTCCCCTACAGGGTCCCAAACAATAACTTG2222 AlaGluGluLysLysTrpValProTyrArgValProAsnAsnAsnLeu 705710715 CCAATCCCTGA ACGTTACCTGTCCCCAGATGCCACGGTCTCCACAGAA2270 ProIleProGluArgTyrLeuSerProAspAlaThrValSerThrGlu 720725730 GTCCGGGCCATTATTTC CTGGATGGAGAAGAACCCCTTTGTGCTGGGT2318 ValArgAlaIleIleSerTrpMetGluLysAsnProPheValLeuGly 735740745750 GCAAATCTGAA CGGTGGTGAGCGGCTTGTGTCTTATCCCTATGACATG2366 AlaAsnLeuAsnGlyGlyGluArgLeuValSerTyrProTyrAspMet 755760765 GCCCGGAC ACCTAGCCAGGAGCAGCTGTTGGCCGAGGCACTGGCAGCT2414 AlaArgThrProSerGlnGluGlnLeuLeuAlaGluAlaLeuAlaAla 770775780 GCCCGCGG AGAAGATGATGACGGGGTGTCTGAGGCCCAGGAGACTCCA2462 AlaArgGlyGluAspAspAspGlyValSerGluAlaGlnGluThrPro 785790795 GATCACGCTAT TTTCCGCTGGCTGGCCATCTCATTTGCCTCCGCCCAT2510 AspHisAlaIlePheArgTrpLeuAlaIleSerPheAlaSerAlaHis 800805810 CTCACCATGACGGAGCC CTACCGGGGAGGGTGCCAGGCCCAGGACTAC2558 LeuThrMetThrGluProTyrArgGlyGlyCysGlnAlaGlnAspTyr 815820825830 ACCAGCGGCAT GGGCATTGTCAACGGGGCCAAGTGGAATCCTCGCTCT2606 ThrSerGlyMetGlyIleValAsnGlyAlaLysTrpAsnProArgSer 835840845 GGGACTTT CAATGACTTTAGCTACCTGCACACAAACTGTCTGGAGCTC2654 GlyThrPheAsnAspPheSerTyrLeuHisThrAsnCysLeuGluLeu 850855860 TCCGTATA CCTGGGCTGTGACAAGTTCCCCCACGAGAGTGAGCTACCC2702 SerValTyrLeuGlyCysAspLysPheProHisGluSerGluLeuPro 865870875 CGAGAATGGGA GAACAACAAAGAAGCGCTGCTCACCTTCATGGAGCAG2750 ArgGluTrpGluAsnAsnLysGluAlaLeuLeuThrPheMetGluGln 880885890 GTGCACCGTGGCATTAA GGGTGTGGTGACAGATGAGCAAGGCATCCCC2798 ValHisArgGlyIleLysGlyValValThrAspGluGlnGlyIlePro 895900905910 ATTGCCAATGC CACCATCTCTGTGAGTGGCATCAACCATGGTGTGAAG2846 IleAlaAsnAlaThrIleSerValSerGlyIleAsnHisGlyValLys 915920925 ACAGCAAG TGGAGGTGACTACTGGCGCATTCTGAACCCGGGTGAGTAC2894 ThrAlaSerGlyGlyAspTyrTrpArgIleLeuAsnProGlyGluTyr 930935940 CGTGTGAC AGCTCACGCAGAGGGCTACACCTCAAGTGCCAAGATCTGC2942 ArgValThrAlaHisAlaGluGlyTyrThrSerSerAlaLysIleCys 945950955 AATGTGGACTA CGATATTGGGGCCACTCAGTGCAACTTCATCCTGGCT2990 AsnValAspTyrAspIleGlyAlaThrGlnCysAsnPheIleLeuAla 960965970 CGATCCAACTGGAAGCG CATTCGGGAGATCTTGGCTATGAACGGGAAC3038 ArgSerAsnTrpLysArgIleArgGluIleLeuAlaMetAsnGlyAsn 975980985990 CGTCCCATTCT CGGAGTTGACCCCTCACGACCCATGACCCCCCAGCAG3086 ArgProIleLeuGlyValAspProSerArgProMetThrProGlnGln 99510001005 CGGCGCA TGCAGCAGCGCCGTCTACAGTACCGGCTCCGCATGAGGGAA3134 ArgArgMetGlnGlnArgArgLeuGlnTyrArgLeuArgMetArgGlu 101010151020 CAGATG CAACTGCGTCGCCTCAATTCTACCGCAGGCCCTGCCACAAGC3182 GlnMetGlnLeuArgArgLeuAsnSerThrAlaGlyProAlaThrSer 102510301035 CCCACTCCT GCCCTTATGCCTCCCCCTTCCCCTACACCAGCCATTACC3230 ProThrProAlaLeuMetProProProSerProThrProAlaIleThr 104010451050 TTGAGGCCCTGGGA AGTTCTACCCACTACCACTGCAGGCTGGGAGGAG3278 LeuArgProTrpGluValLeuProThrThrThrAlaGlyTrpGluGlu 1055106010651070 TCAGAGA CTGAGACCTATACAGAAGTAGTGACAGAGTTTGAGACAGAG3326 SerGluThrGluThrTyrThrGluValValThrGluPheGluThrGlu 107510801085 TAT GGGACTGACCTAGAGGTGGAAGAGATAGAGGAGGAGGAGGAGGAG3374 TyrGlyThrAspLeuGluValGluGluIleGluGluGluGluGluGlu 109010951100 GAG GAGGAAGAGATGGACACAGGCCTTACATTTCCACTCACAACAGTG3422 GluGluGluGluMetAspThrGlyLeuThrPheProLeuThrThrVal 110511101115 GAGAC CTACACAGTGAACTTTGGGGACTTCTGAGACTGGGATCTCAAAGC3472 GluThrTyrThrValAsnPheGlyAspPhe 11201125 CCTGCCCAATTCAAACTAAGGCAGCACCTCCCAAGCCTGTGCCAGCAGACAC ATAGCCAT3532 CAGATGTCCCTGGGGTGGACCCCACTCCCCCAGTGTGGGACATCAAAGCTACCGGGACTC3592 TGCATAGACTCTGGTCTACCCGCCCCAGCTCTTACCTGCCAGCCTTTGGGGGAGGGGCAG3652 GCAAAGGAAGCCAACGTTCAACATCAA TAAAACCAAGCTCATGACACCAAAAAAAAAAAA3712 AAGCGGCCGCGAATTC3728 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1128 amino acids (B) TYPE: amino acid (D ) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: MetAlaProValArgThrAlaSerLeuLeuCysGlyLeuLeuAlaLeu 151015 LeuThrLeuCysProGluGlyAsn ProGlnThrValLeuThrAspAsp 202530 GluIleGluGluPheLeuGluGlyPheLeuSerGluLeuGluThrGln 3540 45 SerProProArgGluAspAspValGluValGlnProLeuProGluPro 505560 ThrGlnArgProArgLysSerLysAlaGlyGlyLysGlnArgAlaAsp 65 707580 ValGluValProProGluLysAsnLysAspLysGluLysLysGlyLys 859095 LysAsp LysGlyProLysAlaThrLysProLeuGluGlySerThrArg 100105110 ProThrLysLysProLysGluLysProProLysAlaThrLysLysPro 115 120125 LysGluLysProProLysAlaThrLysLysProLysGluLysProPro 130135140 LysAlaThrLysLysProLysGluLysProPro LysAlaThrLysArg 145150155160 ProSerAlaGlyLysLysPheSerThrValAlaProLeuGluThrLeu 165170 175 AspArgLeuLeuProSerProSerAsnProSerAlaGlnGluLeuPro 180185190 GlnLysArgAspThrProPheProAsnAlaTrpGlnGlyGlnG lyGlu 195200205 GluThrGlnValGluAlaLysGlnProArgProGluProGluGluGlu 210215220 ThrGluMetProThr LeuAspTyrAsnAspGlnIleGluLysGluAsp 225230235240 TyrGluAspPheLysTyrIleLeuCysGlnLysGlnProArgProThr 245 250255 ProSerArgArgArgLeuTrpProGluArgProGluGluLysThrGlu 260265270 GluProGluGluArgLysGluVal GluProProLeuLysProLeuLeu 275280285 ProProAspTyrGlyAspSerTyrValIleProAsnTyrAspAspLeu 2902953 00 AspTyrTyrPheProHisProProProGlnLysProAspValGlyGln 305310315320 GluValAspGluGluLysGluGluMetLysLysProLysLysGluG ly 325330335 SerSerProLysGluAspThrGluAspLysTrpThrValGluLysAsn 340345350 LysAsp HisLysGlyProArgLysGlyGluGluLeuGluGluGluTrp 355360365 AlaProValGluLysIleLysCysProProIleGlyMetGluSerHis 370 375380 ArgIleGluAspAsnGlnIleArgAlaSerSerMetLeuArgHisGly 385390395400 LeuGlyAlaGlnArgGlyArgLeuAsn MetGlnAlaGlyAlaAsnGlu 405410415 AspAspTyrTyrAspGlyAlaTrpCysAlaGluAspGluSerGlnThr 420425 430 GlnTrpIleGluValAspThrArgArgThrThrArgPheThrGlyVal 435440445 IleThrGlnGlyArgAspSerSerIleHisAspAspPheValThrT hr 450455460 PhePheValGlyPheSerAsnAspSerGlnThrTrpValMetTyrThr 465470475480 AsnGlyTyr GluGluMetThrPheTyrGlyAsnValAspLysAspThr 485490495 ProValLeuSerGluLeuProGluProValValAlaArgPheIleArg 500 505510 IleTyrProLeuThrTrpAsnGlySerLeuCysMetArgLeuGluVal 515520525 LeuGlyCysProValThrProValTyr SerTyrTyrAlaGlnAsnGlu 530535540 ValValThrThrAspSerLeuAspPheArgHisHisSerTyrLysAsp 545550555 560 MetArgGlnLeuMetLysAlaValAsnGluGluCysProThrIleThr 565570575 ArgThrTyrSerLeuGlyLysSerSerArgGlyLeuLysIleT yrAla 580585590 MetGluIleSerAspAsnProGlyAspHisGluLeuGlyGluProGlu 595600605 PheArgTyr ThrAlaGlyIleHisGlyAsnGluValLeuGlyArgGlu 610615620 LeuLeuLeuLeuLeuMetGlnTyrLeuCysGlnGluTyrArgAspGly 625630 635640 AsnProArgValArgAsnLeuValGlnAspThrArgIleHisLeuVal 645650655 ProSerLeuAsnProAspGlyTyr GluValAlaAlaGlnMetGlySer 660665670 GluPheGlyAsnTrpAlaLeuGlyLeuTrpThrGluGluGlyPheAsp 675680 685 IlePheGluAspPheProAspLeuAsnSerValLeuTrpAlaAlaGlu 690695700 GluLysLysTrpValProTyrArgValProAsnAsnAsnLeuProIle 705 710715720 ProGluArgTyrLeuSerProAspAlaThrValSerThrGluValArg 725730735 AlaIle IleSerTrpMetGluLysAsnProPheValLeuGlyAlaAsn 740745750 LeuAsnGlyGlyGluArgLeuValSerTyrProTyrAspMetAlaArg 755 760765 ThrProSerGlnGluGlnLeuLeuAlaGluAlaLeuAlaAlaAlaArg 770775780 GlyGluAspAspAspGlyValSerGluAlaGln GluThrProAspHis 785790795800 AlaIlePheArgTrpLeuAlaIleSerPheAlaSerAlaHisLeuThr 805810 815 MetThrGluProTyrArgGlyGlyCysGlnAlaGlnAspTyrThrSer 820825830 GlyMetGlyIleValAsnGlyAlaLysTrpAsnProArgSerG lyThr 835840845 PheAsnAspPheSerTyrLeuHisThrAsnCysLeuGluLeuSerVal 850855860 TyrLeuGlyCysAsp LysPheProHisGluSerGluLeuProArgGlu 865870875880 TrpGluAsnAsnLysGluAlaLeuLeuThrPheMetGluGlnValHis 885 890895 ArgGlyIleLysGlyValValThrAspGluGlnGlyIleProIleAla 900905910 AsnAlaThrIleSerValSerGly IleAsnHisGlyValLysThrAla 915920925 SerGlyGlyAspTyrTrpArgIleLeuAsnProGlyGluTyrArgVal 9309359 40 ThrAlaHisAlaGluGlyTyrThrSerSerAlaLysIleCysAsnVal 945950955960 AspTyrAspIleGlyAlaThrGlnCysAsnPheIleLeuAlaArgS er 965970975 AsnTrpLysArgIleArgGluIleLeuAlaMetAsnGlyAsnArgPro 980985990 IleLeu GlyValAspProSerArgProMetThrProGlnGlnArgArg 99510001005 MetGlnGlnArgArgLeuGlnTyrArgLeuArgMetArgGluGlnMet 1010 10151020 GlnLeuArgArgLeuAsnSerThrAlaGlyProAlaThrSerProThr 1025103010351040 ProAlaLeuMetProProProSerP roThrProAlaIleThrLeuArg 104510501055 ProTrpGluValLeuProThrThrThrAlaGlyTrpGluGluSerGlu 10601065 1070 ThrGluThrTyrThrGluValValThrGluPheGluThrGluTyrGly 107510801085 ThrAspLeuGluValGluGluIleGluGluGluGluGluGlu GluGlu 109010951100 GluGluMetAspThrGlyLeuThrPheProLeuThrThrValGluThr 1105111011151120 Tyr ThrValAsnPheGlyAspPhe 1125 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: Other (A) DESCRIPTION: linker DNA with sequence complementary to Sequence ID No. 4, termed "ATOS-1" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: CTCTTGCTTGAATTCGGACTA21 (2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: Other (A) DESCRIPTION: linker DNA with sequence complementary to Sequence ID No. 3, termed "ATOS-2" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: TAGTCCGAATTCAAGCAAGAGCACA25 (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: Other (A) DESCRIPTION: linker DNA with sequence complementary to Sequence ID No. 6, termed "ATOS-4" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: CTCTTGCTTAAGCTTGGACTA 21 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: Other (A) DESCRIPTION: linker DNA with sequence complementary to Sequence ID No. 5, termed "ATOS-5" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: TAGTCCAAGCTTAAGCAAGAGCACA25 (2) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 11 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: Other ( A) DESCRIPTION: OSF 5.1 (antigen peptide) segment of mouse OSF-5 from the 116th to the 126th amino acid residue (vi) ORIGINAL SOURCE: (A) ORGANISM: Mus musculus (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: LysProLysGluLysProProLysAlaThrLys 15 10 (2) INFORMATION FOR SEQ ID NO:8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: Other (A) DESCRIPTION: OSF 5.1 (antigen peptide) segment of mouse OSF-5 from the 482nd to the 496th amino acid residue (vi) ORIGINAL SOURCE: (A) ORGANISM: Mus musculus (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: GlyThrGlnGlnMetThrPheTyrGlyAsnValAspLysAspThr 151015 (2) INFORMATION FOR SEQ ID NO:9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: Other (A) DESCRIPTION: OSF 5.1 (antigen peptide) segment of mouse OSF-5 from the 557th to the 571st amino acid residue (vi) ORIGINAL SOURCE: (A) ORGANISM: Mus musculus (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: SerTyrLysAsp MetArgGlnLeuMetLysAlaValAspGluGlu 151015 (2) INFORMATION FOR SEQ ID NO:10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: Other (A) DESCRIPTION: OSF 5.1 (antigen peptide) segment of mouse OSF-5 from the 701st to the 715th amino acid residue (vi) ORIGINAL SOURCE: (A) ORGANISM: Mus musculus (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: TrpAlaAlaGluGluLysLysTrpValProTyrArgValProAsn 151015 (2) INFORMATION FOR SEQ ID NO:11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: Other (A) DESCRIPTION: OSF 5.1 (antigen peptide) segment of mouse OSF-5 from the 872nd to the 886th amino acid residue (vi) ORIGINAL SOURCE: (A) ORGANISM: Mus musculus (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11: ProHisGluSerGluLeuProArgGluTrpGluAsnAsnLysGlu 1510 15 (2) INFORMATION FOR SEQ ID NO:12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 484 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: GluValValThrThrAspSerLeuAspPheArgHisHisSerTyr Lys 151015 AspMetArgGlnLeuMetLysAlaValAsnGluGluCysProThrIle 2025 30 ThrArgThrTyrSerLeuGlyLysSerSerArgGlyLeuLysIleTyr 354045 AlaMetGluIleSerAspAsnProGlyAspHisGluLeuGlyG luPro 505560 GluPheArgTyrThrAlaGlyIleHisGlyAsnGluValLeuGlyArg 657075 80 GluLeuLeuLeuLeuLeuMetGlnTyrLeuCysGlnGluTyrArgAsp 859095 GlyAsnProArgValArgAsnLeuValGlnAspThrArg IleHisLeu 100105110 ValProSerLeuAsnProAspGlyTyrGluValAlaAlaGlnMetGly 115120 125 SerGluPheGlyAsnTrpAlaLeuGlyLeuTrpThrGluGluGlyPhe 130135140 AspIlePheGluAspPheProAspLeuAsnSerValLeuTrpAl aAla 145150155160 GluGluLysLysTrpValProTyrArgValProAsnAsnAsnLeuPro 165170 175 IleProGluArgTyrLeuSerProAspAlaThrValSerThrGluVal 180185190 ArgAlaIleIleSerTrpMetGluLysAsnP roPheValLeuGlyAla 195200205 AsnLeuAsnGlyGlyGluArgLeuValSerTyrProTyrAspMetAla 210215 220 ArgThrProSerGlnGluGlnLeuLeuAlaGluAlaLeuAlaAlaAla 225230235240 ArgGlyGluAspAspAspGlyValSerGlu AlaGlnGluThrProAsp 245250255 HisAlaIlePheArgTrpLeuAlaIleSerPheAlaSerAlaHisLeu 260 265270 ThrMetThrGluProTyrArgGlyGlyCysGlnAlaGlnAspTyrThr 275280285 SerGlyMetGlyIleValAsnGlyAla LysTrpAsnProArgSerGly 290295300 ThrPheAsnAspPheSerTyrLeuHisThrAsnCysLeuGluLeuSer 305310 315320 ValTyrLeuGlyCysAspLysPheProHisGluSerGluLeuProArg 325330335 GluTrpGluAsnAsnLysGl uAlaLeuLeuThrPheMetGluGlnVal 340345350 HisArgGlyIleLysGlyValValThrAspGluGlnGlyIleProIle 355 360365 AlaAsnAlaThrIleSerValSerGlyIleAsnHisGlyValLysThr 370375380 AlaSerGlyGlyAspTyrTrpArgI leLeuAsnProGlyGluTyrArg 385390395400 ValThrAlaHisAlaGluGlyTyrThrSerSerAlaLysIleCysAsn 405 410415 ValAspTyrAspIleGlyAlaThrGlnCysAsnPheIleLeuAlaArg 420425430 SerAsnTrpLys ArgIleArgGluIleLeuAlaMetAsnGlyAsnArg 435440445 ProIleLeuGlyValAspProSerArgProMetThrProGlnGlnArg 450 455460 ArgMetGlnGlnArgArgLeuGlnTyrArgLeuArgMetArgGluGln 465470475480 MetGlnLeuArg (2) INFORMATION FOR SEQ ID NO:13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 434 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13: ArgProGlnGluAspGlyIleSerPheGluTyrHisArgTyrProGlu 1 51015 LeuArgGluAlaLeuValSerValTrpLeuGlnCysAlaAlaValSer 202530 Arg IleTyrThrValGlyArgSerPheGluGlyArgGluLeuLeuVal 354045 LeuGluLeuSerAspAsnProGlyValHisGluProGlyGluProGlu 505560 PheLysTyrIleGlyAsnMetHisGlyAsnGluAlaValGlyArgGlu 65707580 LeuL euIlePheLeuAlaGlnTyrLeuCysAsnGluTyrGlnLysGly 859095 AsnGluThrIleValGlnLeuIleHisAsnThrArgIleHisIleMet 100105110 ProSerLeuAsnProAspGlyPheGluLysAlaAlaSerGlnLeuGly 115120125 G luLeuLysAspTrpPheValGlyArgSerAsnAlaGlnGlyIleAsp 130135140 LeuAsnArgAsnPheProAspLeuAspArgIleValTyrIleAsnGlu 145 150155160 LysGluGlyGlyProAsnAsnHisLeuLeuLysAsnLeuLysLysIle 165170175 ValAspGlnAsnThrLysLeuAlaProGluThrLysAlaValIleHis 180185190 TrpIleMetAspIleProPheValLeuSerAlaAsnLeuHisG lyGly 195200205 AspLeuValAlaAsnTyrProTyrAspGluThrArgSerGlySerAla 210215220 HisGluTyrSerSerCysProAspAspAspIlePheGlnSerLeuAla 225230235240 ArgAlaTyrSerSerPheAsnProProMetSerAspProAsp ArgPro 245250255 ProCysArgLysAsnAspAspAspSerSerPheValGluGlyThrThr 260265 270 AsnGlyAlaAlaTrpTyrSerValProGlyGlyMetGlnAspPheAsn 275280285 TyrLeuSerSerAsnCysPheGluIleThrValGluLeu SerCysGlu 290295300 LysPheProProGluGluThrLeuLysAsnTyrTrpGluAspAsnLys 305310315 320 AsnSerLeuIleSerTyrIleGlnGlnIleHisArgGlyValLysGly 325330335 PheValArgAspLeuGlnGlyAsnProIleAl aAsnAlaThrLeuSer 340345350 ValGluGlyIleAspHisAspValThrSerAlaLysAspGlyAspTyr 355360 365 TrpArgLeuLeuValProGlyAsnTyrLysLeuThrAlaSerAlaPro 370375380 GlyTyrLeuAlaIleAlaLysLysValAlaValProT yrSerProAla 385390395400 ValArgValAspPheGluLeuGluSerPheSerGluArgLysGluGlu 405 410415 GluLysGluGluLeuMetGluTrpTrpLysMetMetSerGluThrLeu 420425430 AsnPhe (2) INFORMATION FOR SEQ ID NO:14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 435 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: ArgLeuGlnGlnGluAspGlyIleSerPheGluTyrHisArgTyrPro 15 1015 GluLeuArgGluAlaLeuValSerValTrpLeuGlnCysThrAlaIle 202530 SerArgIleTyrThrValGly ArgSerPheGluGlyArgGluLeuLeu 354045 ValIleGluLeuSerAspAsnProGlyValHisGluProGlyGluPro 5055 60 GluPheLysTyrIleGlyAsnMetHisGlyAsnGluAlaValGlyArg 65707580 GluLeuLeuIlePheLeuAlaGl nTyrLeuCysAsnGluTyrGlnArg 859095 GlyAsnGluThrIleValAsnLeuIleHisSerThrArgIleHisIle 100 105110 MetProSerLeuAsnProAspGlyPheGluLysAlaAlaSerGlnPro 115120125 GlyGluLeuLysAspTrpPh eValGlyArgSerAsnAlaGlnGlyIle 130135140 AspLeuAsnArgAsnPheProAspLeuAspArgIleValTyrValAsn 145150 155160 GluLysGluGlyGlyProAsnAsnHisLeuLeuLysAsnLeuLysLys 165170175 IleValAspGlnA snSerLysLeuAlaProGluThrLysAlaValIle 180185190 HisTrpIleMetAspIleProPheValLeuSerAlaAsnLeuHisGly 195 200205 GlyAspLeuValAlaAsnTyrProTyrAspGluThrArgSerGlyThr 210215220 AlaHisGluTyrSerSer CysProAspAspAlaIlePheGlnSerLeu 225230235240 AlaArgAlaTyrSerSerPheAsnProValMetSerAspProAsnArg 245250255 ProProCysArgLysAsnAspAspAspSerSerPheValAspGlyThr 260265270 ThrAsn GlyGlyAlaTrpTyrSerValProGlyGlyMetGlnAspPhe 275280285 AsnTyrLeuSerSerAsnCysPheGluIleThrValGluLeuSerCys 2 90295300 GluLysPheProProGluGluThrLeuLysSerTyrTrpGluAspAsn 305310315320 LysAs nSerLeuIleAsnTyrLeuGluGlnIleHisArgGlyValLys 325330335 GlyPheValArgAspLeuGlnGlyAsnProIleAlaAsnAlaThrIle 340345350 SerValAspGlyIleAspHisAspValThrSerAlaLysAspGlyAsp 355360365 T yrTrpArgLeuLeuValProGlyAsnTyrLysLeuThrAlaSerAla 370375380 ProGlyTyrLeuAlaIleThrLysLysValAlaValProPheSerPro 385 390395400 AlaValGlyValAspPheGluLeuGluSerPheSerGluArgLysGlu 405410415 GluGluLysGluGluLeuMetGluTrpTrpLysMetMetSerGluThr 420425430 LeuAsnPhe 435 (2) INFORMATION FOR SEQ ID NO:15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 435 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15: ArgLeuGlnGlnGluAspGlyIleSerPheGluTyrHisArgTyrPro 15 1015 GluLeuArgGluAlaLeuValSerValTrpLeuGlnCysThrAlaIle 202530 SerArgIleTyrThrValGlyAr gThrPheGluGlyArgGluLeuLeu 354045 ValIleGluLeuSerAspAsnProGlyValHisGluProGlyGluPro 5055 60 GluPheLysTyrIleGlyAsnMetHisGlyAsnGluAlaValGlyArg 65707580 GluLeuLeuIlePheLeuAlaGln TyrLeuCysAsnGluTyrGlnArg 859095 GlyAsnGluThrIleValAsnLeuIleHisSerThrArgIleHisIle 100 105110 MetProSerLeuAsnProAspGlyPheGluLysAlaAlaSerGlnPro 115120125 GlyGluLeuLysAspTrpPhe ValGlyArgSerAsnAlaGlnGlyIle 130135140 AspLeuAsnArgAsnPheProAspLeuAspArgIleValTyrValAsn 145150 155160 GluLysGluGlyGlyProAsnAsnHisLeuLeuLysAsnLeuLysLys 165170175 IleValAspGlnAsn SerLysLeuAlaProGluThrLysAlaValIle 180185190 HisTrpIleMetAspIleProPheValLeuSerAlaAsnLeuHisGly 195 200205 GlyAspLeuValAlaAsnTyrProTyrAspGluThrArgSerGlyThr 210215220 AlaHisGluTyrSerSerCy sProAspAspAlaIlePheGlnSerLeu 225230235240 AlaArgAlaTyrSerSerPheAsnProValMetSerAspProAsnArg 245250255 ProProCysArgLysAsnAspAspAspSerSerPheValAspGlyThr 260265270 ThrAsnG lyGlyAlaTrpTyrSerValProGlyGlyMetGlnAspPhe 275280285 AsnTyrLeuSerSerAsnCysPheGluIleThrValGluLeuSerCys 290 295300 GluLysPheProProGluGluThrLeuLysSerTyrTrpGluAspAsn 305310315320 LysAsn SerLeuIleAsnTyrLeuGluGlnIleHisArgGlyValLys 325330335 GlyPheValArgAspLeuGlnGlyAsnProIleAlaAsnAlaThrIle 340345350 SerValAspGlyIleAspHisAspValThrSerAlaLysAspGlyAsp 355360365 Tyr TrpArgLeuLeuValProGlyAsnTyrLysLeuThrAlaSerAla 370375380 ProGlyTyrLeuAlaIleThrLysLysValAlaValProPheSerPro 385 390395400 AlaValGlyValAspPheGluLeuGluSerPheSerGluArgLysGlu 405410415 GluGluLysGluGluLeuMetGluTrpTrpLysMetMetSerGluThr 420425430 LeuAsnPhe 435 (2) INFORMATION FOR SEQ ID NO:16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 435 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16: ArgLeuGlnGlnGluAspGlyIleSerPheGluTyrHisArgTyrPro 15 1015 GluLeuArgGluAlaLeuValSerValTrpLeuGlnCysThrAlaIle 202530 SerArgIleTyrThrValGlyArg SerPheGluGlyArgGluLeuLeu 354045 ValIleGluLeuSerAspAsnProGlyValHisGluProGlyGluPro 5055 60 GluPheLysTyrIleGlyAsnMetHisGlyAsnGluAlaValGlyArg 65707580 GluLeuLeuIlePheLeuAlaGlnTy rLeuCysAsnGluTyrGlnLys 859095 GlyAsnGluThrIleValAsnLeuIleHisSerThrArgIleHisIle 100 105110 MetProSerLeuAsnProAspGlyPheGluLysAlaAlaSerGlnPro 115120125 GlyGluLeuLysAspTrpPheVa lGlyArgSerAsnAlaGlnGlyIle 130135140 AspLeuAsnArgAsnPheProAspLeuAspArgIleValTyrValAsn 145150 155160 GluLysGluGlyGlyProAsnAsnHisLeuLeuLysAsnMetLysLys 165170175 IleValAspGlnAsnT hrLysLeuAlaProGluThrLysAlaValIle 180185190 HisTrpIleMetAspIleProPheValLeuSerAlaAsnLeuHisGly 195 200205 GlyAspLeuValAlaAsnTyrProTyrAspGluThrArgSerGlySer 210215220 AlaHisGluTyrSerSerSer ProAspAspAlaIlePheGlnSerLeu 225230235240 AlaArgAlaTyrSerSerPheAsnProAlaMetSerAspProAsnArg 245250255 ProProCysArgLysAsnAspAspAspSerSerPheValAspGlyThr 260265270 ThrAsnGly GlyAlaTrpTyrSerValProGlyGlyMetGlnAspPhe 275280285 AsnTyrLeuSerSerAsnCysPheGluIleThrValGluLeuSerCys 290 295300 GluLysPheProProGluGluThrLeuLysThrTyrTrpGluAspAsn 305310315320 LysAsnSe rLeuIleSerTyrLeuGluGlnIleHisArgGlyValLys 325330335 GlyPheValArgAspLeuGlnGlyAsnProIleAlaAsnAlaThrIle 340345350 SerValGluGlyIleAspHisAspValThrSerAlaLysAspGlyAsp 355360365 TyrT rpArgLeuLeuIleProGlyAsnTyrLysLeuThrAlaSerAla 370375380 ProGlyTyrLeuAlaIleThrLysLysValAlaValProTyrSerPro 385 390395400 AlaAlaGlyValAspPheGluLeuGluSerPheSerGluArgLysGlu 405410415 GluGluLysGluGluLeuMetGluTrpTrpLysMetMetSerGluThr 420425430 LeuAsnPhe 435 (2) INFORMATION FOR SEQ ID NO:17: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 438 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17: ValThrPheArgHisHisArgTyrAspAspLeuValArgThrLeuTyr 15 1015 LysValGlnAsnGluCysProGlyIleThrArgValTyrSerIleGly 202530 ArgSerValGluGlyArgHisLeuTy rValLeuGluPheSerAspHis 354045 ProGlyIleHisGluProLeuGluProGluValLysTyrValGlyAsn 5055 60 MetHisGlyAsnGluAlaLeuGlyArgGluLeuMetLeuGlnLeuSer 65707580 GluPheLeuCysGluGluPheArgAsn ArgAsnGlnArgIleValGln 859095 LeuIleGlnAspThrArgIleHisIleLeuProSerMetAsnProAsp 100 105110 GlyTyrGluValAlaAlaAlaGlnGlyProAsnLysProGlyTyrLeu 115120125 ValGlyArgAsnAsnAlaAsnGly ValAspLeuAsnArgAsnPhePro 130135140 AspLeuAsnThrTyrIleTyrTyrAsnGluLysTyrGlyGlyProAsn 145150 155160 HisHisLeuProLeuProAspAsnTrpLysSerGlnValGluProGlu 165170175 ThrArgAlaValIleArg TrpMetHisSerPheAsnPheValLeuSer 180185190 AlaAsnLeuHisGlyGlyAlaValValAlaAsnTyrProTyrAspLys 195 200205 SerPheGluHisArgValArgGlyValArgArgThrAlaSerThrPro 210215220 ThrProAspAspLysLeuPheGl nLysLeuAlaLysValTyrSerTyr 225230235240 AlaHisGlyTrpMetPheGlnGlyTrpAsnCysGlyAspTyrPhePro 245250255 AspGlyIleThrAsnGlyAlaSerTrpTyrSerLeuSerLysGlyMet 260265270 GlnAspPheA snTyrLeuHisThrAsnCysPheGluIleThrLeuGlu 275280285 LeuSerCysAspLysPheProProGluGluGluLeuGlnArgGluTrp 290 295300 LeuGlyAsnArgGluAlaLeuIleGlnPheLeuGluGlnValHisGln 305310315320 GlyIleLys GlyMetValLeuAspGluAsnTyrAsnAsnLeuAlaAsn 325330335 AlaValIleSerValSerGlyIleAsnHisAspValThrSerGlyAsp 340345350 HisGlyAspTyrPheArgLeuLeuLeuProGlyIleTyrThrValSer 355360365 AlaThr AlaProGlyTyrAspProGluThrValThrValThrValGly 370375380 ProAlaGluProThrLeuValAsnPheHisLeuLysArgSerIlePro 385 390395400 GlnValSerProValArgArgAlaProSerArgArgHisGlyValArg 405410415 AlaLysValGlnProGlnAlaArgLysLysGluMetGluMetArgGln 420425430 LeuGlnArgGlyProAla 435 (2) INFORMATION FOR SEQ ID NO:18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 132 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18: PheHisTyrValCysArgTyrGlyGlyGluSerAspProGlyHisGlu 15 1015 GluProGluTyrHisGlyAsnGluGlyArgGluLeuLeuLeuCysGlu 202530 AsnLeuThrArgIleHis ProSerAsnProAspGlyGluAlaAlaGly 354045 GlyAspPheProAspLeuGluProAsnLeuGluAlaIleTrpPheVal 50 5560 LeuAlaAsnLeuGlyGlyTyrProTyrAspProAspPheLeuAlaMet 65707580 GlyAsnGlyTrpAspPheTy rLeuAsnCysGluLeuCysLysPhePro 859095 GluLeuTrpAsnLeuGlnHisGlyLysGlyValAspAlaAsnAlaSer 100 105110 ValGlyIleHisValGlyAspTyrArgLeuProGlyTyrAlaAlaGly 115120125 TyrValPheLeu 130 (2) INFORMATION FOR SEQ ID NO:19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 109 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19: AlaTrpCysAlaGluAspGluSerGlnThrGlnTrpIleGluValAsp 151015 ThrArgArgThrThrArgPheThrGlyValIleThrGlnGlyArgAsp 202530 SerSerIleHisAspAspPheValThrThrPhePheValGlyPheSer 354045 AsnAspSerGlnThrTrpValMetTyrThrAsnGlyTyrGluGluMet 505560 ThrPheTyrGlyAsnValAspLysAspThrProValLeuSerGluLeu 65707580 ProGluProValValAlaArgPheIleArgIleTyrProLeuThrTrp 859095 AsnGlySerLeuCysMetArgLeuGluValLeuGlyCys 100105 (2) INFORMATION FOR SEQ ID NO:20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 109 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20: AlaTrpSerThrLysGluProPheSerTrp IleLysValAspLeuLeu 151015 AlaProMetIleIleHisGlyIleLysThrGlnGlyAlaArgGlnLys 20 2530 PheSerSerLeuTyrIleSerGlnPheIleIleMetTyrSerLeuAsp 354045 GlyLysLysTrpGlnThrTyrArgGlyAs nSerThrGlyThrLeuMet 505560 ValPhePheGlyAsnValAspSerSerGlyIleLysHisAsnIlePhe 65707 580 AsnProProIleIleAlaArgTyrIleArgLeuHisProThrHisTyr 859095 SerIleArgSerThrLeuArgMet GluLeuMetGlyCys 100105 (2) INFORMATION FOR SEQ ID NO:21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 109 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21: AlaTrpArgP roGlnValAsnAsnProLysGluTrpLeuGlnValAsp 151015 PheGlnLysThrMetLysValThrGlyValThrThrGlnGlyValLys 202530 SerLeuLeuThrSerMetTyrValLysGluPheLeuIleSerSerSer 354045 GlnAspGly HisGlnTrpThrLeuPhePheGlnAsnGlyLysValLys 505560 ValPheGlnGlyAsnGlnAspSerPheThrProValValAsnSerLeu 65 707580 AspProProLeuLeuThrArgTyrLeuArgIleHisProGlnSerTrp 859095 ValH isGlnIleAlaLeuArgMetGluValLeuGlyCys 100105 (2) INFORMATION FOR SEQ ID NO:22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 115 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22: AlaTrpSerValGluLysLeuAlaAlaGluPheAlaSerLysProTrp 151015 IleGlnValAspMetGlnLysGluValIleIleThr GlyIleGlnThr 202530 GlnGlyAlaLysHisTyrLeuLysSerCysTyrThrThrGluPheTyr 3540 45 ValAlaTyrSerSerAsnGlnIleAsnTrpGlnIlePheLysGlyAsn 505560 SerThrArgAsnValMetTyrPheAsnGlyAsnSerAspAlaSe rThr 65707580 IleLysGluAsnGlnPheAspProProIleValAlaArgTyrIleArg 8590 95 IleSerProThrArgAlaTyrAsnArgProThrLeuArgLeuGluLeu 100105110 GlnGlyCys 115 (2) INFORMATION FOR SEQ ID NO:23: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 111 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23: AlaTrpGlnAlaLysAlaAsnAsnAsnLysGlnTrpLeuGluIleAsp 15 1015 LeuLeuLysIleLysLysIleThrAlaIleIleThrGlnGlyCysLys 202530 SerLeuSerSer GluMetTyrValLysSerTyrThrIleHisTyrSer 354045 GluGlnGlyValGluTrpLysProTyrArgLeuLysSerSerMetVal 50 5560 AspLysIlePheGluGlyAsnThrAsnThrLysGlyHisValLysAsn 65707580 PhePheAsnProPr oIleIleSerArgPheIleArgValIleProLys 859095 ThrTrpAsnGlnSerIleThrLeuArgLeuGluLeuPheGlyCys 1 00105110 (2) INFORMATION FOR SEQ ID NO:24: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 110 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24: AlaTrpHisAlaSerAsnTyrAspS erLysProTrpIleGlnValAsn 151015 LeuLeuArgLysMetArgValSerGlyValMetThrGlnGlyAlaSer 20 2530 ArgAlaGlyArgAlaGluTyrLeuLysThrPheLysValAlaTyrSer 354045 LeuAspGlyArgLysPheGluPhe IleGlnAspGluSerGlyGlyAsp 505560 LysGluPheLeuGlyAsnLeuAspAsnAsnSerLeuLysValAsnMet 6570 7580 PheAsnProThrLeuGluAlaGlnTyrIleArgLeuTyrProValSer 859095 CysHisArgGlyCysThrL euArgPheGluLeuLeuGlyCys 100105110 (2) INFORMATION FOR SEQ ID NO:25: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 109 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25: AlaTrpThrAlaGlnSerAsnSerAlaLysGluTrpLeuGlnValAsp 151015 LeuGlyThrGlnArgGlnValThrGlyIleIle ThrGlnGlyAlaArg 202530 AspPheGlyHisIleGlnTyrValGluSerTyrLysValAlaHisSer 3540 45 AspAspGlyValGlnTrpThrValTyrGluGluGlnGlySerSerLys 505560 ValPheGlnGlyAsnLeuAspAsnAsnSerHisLysLysAs nIlePhe 65707580 GluLysProPheMetAlaArgTyrValArgValLeuProValSerTrp 8590 95 HisAsnArgIleThrLeuArgLeuGluLeuLeuGlyCys 100105 (2) INFORMATION FOR SEQ ID NO:26: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 104 amino acids (B) TYPE: amino acid ( D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26: AlaTrpCysSerSerIleValAspThrAsnGlnTyrIleValAlaGly 151015 CysGluValProA rgThrPheMetCysValAlaLeuGlnGlyArgGly 202530 AspHisAspGlnTrpValThrSerTyrLysIleArgTyrSerLeuAsp 35 4045 AsnValThrTrpSerGluTyrArgAsnGlyAlaAlaIleThrGlyVal 505560 ThrAspArgAsnThrValVal AsnHisPhePheAspThrProIleArg 65707580 AlaArgSerIleAlaIleHisProLeuThrTrpAsnAsnHisIleSer 859095 LeuArgCysGluPheTyrThrGln 100 (2) INFORMATION FOR SEQ ID NO:27: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 41 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear ( ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27: AlaTrpTrpValAspLeuGlyThrGlnGlyTyrValPheTyrSerAsp 151015 TrpTyrPheGlyAsnAspAsnPh eProAlaArgTyrIleArgIlePro 202530 TrpLeuArgLeuGluLeuLeuGlyCys 3540