§
PatentCue
Patent Intelligence
Join Waitlist Sign In
← Back to Patent Explorer
US5480797A Expired / Lapsed US ⏱️ 0.0 years remaining

Augmenter of liver regeneration (ALR)

Assignee: Toyobo Co Ltd
•
Filing: 1994-02-16
•
Granted: 1996-01-02
•
Expires: 2014-02-16
📄 Original PDF 🌐 Google Patents 🔄 Sync Now

Abstract

Abstract A full-length cDNA clone has been isolated encoding a purified augmenter of liver regeneration (ALR) polypeptide prepared from the cytosol of livers from weanling rats. The 1.2 kb cDNA includes a 299 bp 5'-untranslated region, a 375 bp coding region and a 551 bp 3'-untranslated region. The cDNA encodes a protein consisting of 125 amino acids. The molecular weight of ALR calculated from the cDNA was 15,081 which is consistent with the size estimated by SDS-PAGE under reducing conditions. The molecular weight of the purified native ALR estimated by SDS-PAGE under non-reducing conditions is about 30,000, apparently with a homodimer structure. The recombinant ALR produced by expression of the cDNA in cultured cells was tested in vivo in the canine Eck's fistula model and found to have a potency equivalent to the purified native ALR. The most obvious immediate clinical use for the augmenter of liver regeneration is in the treatment of hepatic failure.

Inventors

  • 👤 Antonio T. Francavilla
  • 👤 Michio Hagiya
  • 👤 Thomas E. Starzl

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-09 13:24
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; A; A61; A61K; A61K38/00

Claims Analysis

Total Claims: 11
Claim 1
Independent Claim 25 words
An isolated DNA comprising a nucleotide sequence encoding mammalian augmenter of liver regeneration, wherein said DNA has the nucleotide sequence of SEQ ID NO: 1.
Claim 2
Dependent Claim 25 words
An isolated DNA comprising a nucleotide sequence encoding mammalian augmenter of liver regeneration, wherein said DNA has the nucleotide sequence of SEQ ID NO: 1.
Claim 3
Dependent Claim 24 words
An isolated DNA comprising a nucleotide sequence encoding mammalian augmenter of liver regeneration., wherein said DNA has the nucleotide sequence of SEQ ID NO:3.
Claim 4
Dependent Claim 24 words
An isolated DNA comprising a nucleotide sequence encoding mammalian augmenter of liver regeneration., wherein said DNA has the nucleotide sequence of SEQ ID NO:3.
Claim 5
Dependent Claim 50 words
A recombinant expression vector containing an exogenous DNA sequence encoding mammalian augmenter of liver regeneration wherein the vector is capable of expressing augmenter of liver regeneration in a transformed microorganism or host cell and wherein said augmenter of liver regeneration has the amino acid sequence described in SEQ ID NO:2.
Claim 6
Dependent Claim 50 words
A recombinant expression vector containing an exogenous DNA sequence encoding mammalian augmenter of liver regeneration wherein the vector is capable of expressing augmenter of liver regeneration in a transformed microorganism or host cell and wherein said augmenter of liver regeneration has the amino acid sequence described in SEQ ID NO:2.
Claim 7
Dependent Claim 20 words
A microorganism transformed with the vector of claim 3, wherein said microorganism is capable of expressing augmenter of liver regeneration.
Claim 8
Dependent Claim 12 words
The transformed microorganism of claim 4, wherein said microorganism is E. coli.
Claim 9
Dependent Claim 11 words
The transformed microorganism of claim 4, wherein said microorganism is yeast.
Claim 10
Dependent Claim 23 words
A host cell capable of expressing augmenter of liver regeneration obtained by transforming a mammalian cell line with said vector of claim 3.
Claim 11
Dependent Claim 20 words
The host cell of claim 7, wherein said cell is selected from the group consisting of COS-1, CHO and C127.

References Cited (Backward Citations)

US4341765A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1999-01-01 1999-07-03 2000-01-02 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2003-01-01 2003-07-03 2004-01-02 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2007-01-01 2007-07-03 2008-01-02 $7700.00 Window Closed / Lapsed

Assigned Categories & Tags

No categories assigned to this patent yet.

Assign Category Tag