US5521084A
Expired / Lapsed
US
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Bovine heat shock promoter and uses thereof
Assignee: Inverness Medical Biostar Inc
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Filing: 1992-11-10
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Granted: 1996-05-28
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Expires: 2012-11-10
Abstract
Abstract A novel expression system using the heat-inducible bovine hsp70A promoter and associated cis-acting elements is disclosed. The system provides for the continuous production of a highly pure, authentic protein, substantially free of infectious viral and cellular protein contaminants.
Inventors
- 👤 Jacek Kowalski
- 👤 Scott Gilbert
- 👤 Timothy J. Zamb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 21:08
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; A; A61; A61K; A61K39/00; C07K2319/00; C07K2319/01; C07K2319/02; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16711; C12N2710/16722; C12N2830/00; C12N2830/001; C12N2830/002; C12N2830/80; C12N2830/85
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
190 words
A Madin-Darby bovine kidney (MDBK) cell transformed with a recombinant expression construct comprising: (a) a nucleic acid molecule that comprises a coding sequence for a selected polypeptide; and (b) hsp70 control sequences operably linked to said nucleic acid molecule, whereby said coding sequence will be transcribed and translated when in a host cell to produce said selected polypeptide, and at least one of said control sequences is heterologous to said coding sequence, wherein said hsp70 control sequences comprise (i) a bovine hsp70A promoter selected from the group consisting of a (1) a promoter which comprises the nucleotide sequence depicted at nucleotide positions 3.50 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1); (2) a promoter which comprises the nucleotide sequence depicted at nucleotide positions 265 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1) and (3) a promoter which comprises the nucleotide sequence depicted at nucleotide positions 1 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1), (ii) a bovine 5'-untranslated region positioned downstream of said promoter, and (iii) a 3'-untranslated region positioned downstream of said coding sequence,
Claim 2
Dependent Claim
190 words
A Madin-Darby bovine kidney (MDBK) cell transformed with a recombinant expression construct comprising: (a) a nucleic acid molecule that comprises a coding sequence for a selected polypeptide; and (b) hsp70 control sequences operably linked to said nucleic acid molecule, whereby said coding sequence will be transcribed and translated when in a host cell to produce said selected polypeptide, and at least one of said control sequences is heterologous to said coding sequence, wherein said hsp70 control sequences comprise (i) a bovine hsp70A promoter selected from the group consisting of a (1) a promoter which comprises the nucleotide sequence depicted at nucleotide positions 3.50 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1); (2) a promoter which comprises the nucleotide sequence depicted at nucleotide positions 265 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1) and (3) a promoter which comprises the nucleotide sequence depicted at nucleotide positions 1 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1), (ii) a bovine 5'-untranslated region positioned downstream of said promoter, and (iii) a 3'-untranslated region positioned downstream of said coding sequence,
Claim 3
Dependent Claim
33 words
The MDBK cell of claim 1 wherein said bovine hsp70A promoter comprises the nucleotide sequence depicted at nucleotide positions 350 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1).
Claim 4
Dependent Claim
33 words
The MDBK cell of claim 1 wherein said bovine hsp70A promoter comprises the nucleotide sequence depicted at nucleotide positions 265 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1).
Claim 5
Dependent Claim
33 words
The MDBK cell of claim 1 wherein said bovine hsp70A promoter comprises the nucleotide sequence depicted at nucleotide positions 1 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1).
Claim 6
Dependent Claim
15 words
The MDBK cell of claim 1 wherein said 3'-untranslated region is an hsp70 3'-untranslated region.
Claim 7
Dependent Claim
17 words
The MDBK cell of claim 5 wherein said hsp70 3'-untranslated region comprises a human hsp70 3'-untranslated region.
Claim 8
Dependent Claim
17 words
The MDBK cell of claim 5 wherein said hsp70 3'-untranslated region comprises a Drosophila hsp70 3'-untranslated region.
Claim 9
Dependent Claim
17 words
The MDBK cell of claim 5 wherein said hsp70 3'-untranslated region comprises a bovine hsp70 3'-untranslated region.
Claim 10
Dependent Claim
20 words
The MDBK cell of claim 1 wherein said coding sequence encodes an immunogenic bovine herpesvirus type 1 (BHV-1) gIII polypeptide.
Claim 11
Dependent Claim
20 words
The MDBK cell of claim 1 wherein said coding sequence encodes an immunogenic bovine herpesvirus type 1 (BHV-1) gIV polypeptide.
Claim 12
Dependent Claim
20 words
The MDBK cell of claim 1 wherein said coding sequence encodes an immunogenic bovine viral diarrhea virus (BVDV) gp53 polypeptide.
Claim 13
Dependent Claim
109 words
A Madin-Darby bovine kidney (MDBK) cell transformed with a recombinant expression construct comprising: (a) a nucleic acid molecule that comprises a coding sequence for a selected polypeptide; and (b) hsp70 control sequences operably linked to said nucleic acid molecule and a 3'-untranslated region positioned downstream of said coding sequence, whereby said coding sequence will be transcribed and translated when in a host cell to produce said selected polypeptide, and at least one of said control sequences is heterologous to said coding sequence, wherein said hsp70 control sequences comprise the nucleotide sequence depicted at nucleotide positions 1 to 666, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1).
Claim 14
Dependent Claim
109 words
A Madin-Darby bovine kidney (MDBK) cell transformed with a recombinant expression construct comprising: (a) a nucleic acid molecule that comprises a coding sequence for a selected polypeptide; and (b) hsp70 control sequences operably linked to said nucleic acid molecule and a 3'-untranslated region positioned downstream of said coding sequence, whereby said coding sequence will be transcribed and translated when in a host cell to produce said selected polypeptide, and at least one of said control sequences is heterologous to said coding sequence, wherein said hsp70 control sequences comprise the nucleotide sequence depicted at nucleotide positions 1 to 666, inclusive, of the upper strand of FIG. 2 (SEQ ID NO:1).
Claim 15
Dependent Claim
15 words
The MDBK cell of claim 12 wherein said 3'-untranslated region is an hsp70 3'-untranslated region.
Claim 16
Dependent Claim
17 words
The MDBK cell of claim 13 wherein said hsp70 3'-untranslated region comprises a human hsp70 3'-untranslated region.
Claim 17
Dependent Claim
17 words
The MDBK cell of claim 13 wherein said hsp70 3'-untranslated region comprises a Drosophila hsp70 3'-untranslated region.
Claim 18
Dependent Claim
17 words
The MDBK cell of claim 13 wherein said hsp70 3'-untranslated region comprises a bovine hsp70 3'-untranslated region.
Claim 19
Dependent Claim
20 words
The MDBK cell of claim 14 wherein said coding sequence encodes an immunogenic bovine herpesvirus type 1 (BHV-1) gIII polypeptide.
Claim 20
Dependent Claim
20 words
The MDBK cell of claim 14 wherein said coding sequence encodes an immunogenic bovine herpesvirus type 1 (BHV-1) gIV polypeptide.
Claim 21
Dependent Claim
20 words
The MDBK cell of claim 14 wherein said coding sequence encodes an immunogenic bovine viral diarrhea virus (BVDV) gp53 polypeptide.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-05-28 | 1999-11-27 | 2000-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-05-28 | 2003-11-27 | 2004-05-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-05-28 | 2007-11-27 | 2008-05-28 | $7700.00 | Window Closed / Lapsed |